FastQCFastQC Report
Fri 10 Feb 2017
ERR1633269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785034
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40060.5102963693292265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30600.3897920344851306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17430.2220286000351577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT250.005495294529.626
CGGTCAC400.001930644623.12530
CGCGTAG400.001930644623.12528
GTATCAA18350.022.7847421
TTCGCCG1005.329639E-1022.224
GGTATCA7400.022.0000021
GCCGGCA1205.16593E-918.515
TGCTCGC1409.458745E-1118.510
CCGCTGA1351.1496013E-917.81481626
CCGCTCT1351.1496013E-917.81481628
ACGGTAC752.0662148E-417.2666663
GCTTCGC1301.3909812E-817.07692322
TAGACTG1352.2160748E-816.4444455
CTTAGAC904.4430686E-516.4444453
CGCTGAA1504.66207E-916.03333327
CGCTCTC1504.66207E-916.03333329
TCCCCGC1659.713403E-1015.6969723
TATCAAC27200.015.3033092
ATCAACG26900.015.2676583
TCAACGC27400.015.2591244
Produced by FastQC (version 0.11.5)