FastQCFastQC Report
Fri 10 Feb 2017
ERR1633268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903056
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85650.9484461650218813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.6726050211725519No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC37900.41968604383338354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33870.37505979695611347No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA31690.3509195443029004No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG29510.3267792916496873No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC29250.32390017894792794No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC28230.31260519834871814No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG28010.3101690260626141No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC27490.3044108006590953No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT26690.2955519923459896No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT25800.2856965680976595No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG25550.282928190499814No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG25170.2787202565510888No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG24790.2745123226023635No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG23670.26210999096401555No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT23330.2583449974309456No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA22200.24583193068868375No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG19970.22113800251590157No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT19950.22091653230807393No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC19950.22091653230807393No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC19900.2203628567885048No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG19820.21947697595719423No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT19550.21648712815152105No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG19350.21427242607324465No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC19170.21227919420279584No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC19110.21161478357931293No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA19020.2106181676440885No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA18500.2048599422405698No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT17910.19832657110965432No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG17910.19832657110965432No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT17730.19633333923920554No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA17530.19411863716092911No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG17000.18824967665349657No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA16990.18813894154958274No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG16620.18404174270477136No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC16230.17972307365213233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.17828351730125264No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT15670.17352190783295832No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT15600.17274676210556156No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT15270.16909250367640544No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTA14930.16532751014333552No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA14870.16466309951985258No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC14700.16278060275331763No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT14500.1605659006750412No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC14210.15735458266154037No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC14180.1570223773497989No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC14130.1564687018302298No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACA14050.15558282099891924No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG13960.15458620506369483No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA13420.14860650945234846No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA13290.1471669531014688No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACC13260.14683474778972733No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13200.14617033716624442No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGC13130.14539519143884763No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT13070.14473078081536472No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT12960.14351269467231267No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG12910.14295901915274356No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG12900.14284828404882974No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG12820.14196240321751918No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC12550.138972555411846No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG12380.137090058645311No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTAC12320.1364256480218281No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG12280.13598270760617281No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT12280.13598270760617281No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT12260.13576123739834517No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA12240.13553976719051752No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC11930.13210697896918908No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT11840.13111036303396467No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA11370.12590581315001506No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11270.12479846211087685No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG11200.12402331638348009No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG11150.12346964086391099No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG11120.12313743555216951No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA11080.12269449513651423No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTA10710.11859729629170285No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT10550.1168255346290817No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT10480.11605038890168495No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA10420.11538597827820202No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA10330.11438936234297763No Hit
AAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGAC10150.11239613047252883No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT10100.11184245495295972No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGC9970.11040289860208005No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9950.1101814283942524No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA9750.10796672631597597No Hit
AAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCT9610.10641643486118248No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC9600.10630569975726865No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCAC9560.10586275934161336No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGG9510.10530908382204425No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA9100.10076894456157758No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9090.10065820945766375No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT9070.10043673924983612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG200.001841727337.08
GGTACGC250.00549572829.5999989
CTAGTGC250.00549572829.5999989
TTGCGGT1900.027.26315935
CGGGGCG358.8679156E-426.42857217
CATTACG509.088273E-625.8999982
TGCGGTA2150.024.95348736
GGTATCA15800.024.8227841
CCGCCCC601.3368772E-624.66666629
CGCTCTC2800.023.12500229
GGATAGT400.001930868623.1256
CGCTACC400.001930868623.12528
TTCGCTA400.001930868623.12526
GTATCAA42300.022.8735241
CGGCGCC652.6817888E-622.7692323
CCGCTCT2850.022.71929728
AAGCCGC753.7389327E-722.19999916
CTACACT502.7014813E-422.1999994
GACCGTT855.1775714E-821.7647067
GCCGCTC2900.021.68965527
Produced by FastQC (version 0.11.5)