FastQCFastQC Report
Fri 10 Feb 2017
ERR1633267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831936
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216012.5964737672128626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161851.9454621509346872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88311.0614999230710056No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.5107364028002154No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA39250.471791099315332No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA34240.41157012077852145No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG32040.3851257789060697No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG27240.3274290330025386No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA24200.290887760596969No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.27946861297022846No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21600.2596353565658897No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT21110.2537454804215709No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19320.23222940226171243No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC17960.21588199092237864No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17510.21047292099392262No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17360.20866989768443725No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16860.20265981998615276No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16750.20133760289253022No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16500.19833256404338798No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15950.19172147857527502No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15310.1840285791214709No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC15120.18174474959612277No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA15040.18078313716439726No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14900.1791003154088776No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.17405185014231864No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14260.17140741595507347No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14070.16912358642972536No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14030.16864278021386261No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC13860.16659935379644586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.16551753981075468No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13560.16299330717747518No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.16070947765212706No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13300.15986806677436724No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13020.15650242326332794No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12840.15433879529194555No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12760.15337718286022003No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT12590.1513337564428033No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12340.14832871759366106No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG12260.14736710516193552No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12230.14700650050003847No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12220.14688629894607277No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12010.14436206631279327No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11960.14376105854296486No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11880.14279944611123932No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11860.14255904300330796No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11850.14243884144934227No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC11580.13919339949226864No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11540.1387125932764059No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11370.13666916685898917No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA10760.12933687206708208No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10750.1292166705131164No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10540.12669243787983692No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10510.12633183321793984No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10490.12609143011000848No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10420.12525001923224865No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10410.12512981767828293No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG10410.12512981767828293No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10290.12368739903069467No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10130.12176417416724364No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9980.1199611508577583No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9620.11563389491499347No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9530.11455208092930225No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9300.1117874451880914No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9120.10962381721670898No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9110.10950361566274328No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9100.1093834141087776No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9030.10854200323101777No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG8820.10601777059773829No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8420.1012097084391107No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8410.10108950688514501No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG8370.10060870066928224No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8340.10024809600738518No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.10012789445341949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACT200.001841643837.04
TATACAG200.001841643837.05
TGCGGCT303.598631E-430.83333417
AACCGCG250.005495480729.67
CTACTAA250.005495480729.61
CGCGAGT250.005495480729.618
CGATCTA250.005495480729.617
GTAACCG250.005495480729.620
TAGCAAC358.8673277E-426.428571
GCAATTT358.8673277E-426.4285734
ACAACGC451.322783E-424.6666663
GGCTCGC451.322783E-424.66666619
CTAGTGG701.9215986E-723.7857139
GCGGTTC400.001930741223.12528
CTTCCCT400.001930741223.12535
CAATTTG400.001930741223.12535
AGCGGTT400.001930741223.12527
ATACGGG400.001930741223.1253
ACTAGTG1950.022.7692328
GTCACTG652.6813668E-622.7692329
Produced by FastQC (version 0.11.5)