FastQCFastQC Report
Fri 10 Feb 2017
ERR1633266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785296
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33680.42888286709724743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.2958120250198651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13210.1682168252480593No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA8800.11205965648621666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA451.3226905E-424.66666827
GTATCAA17800.022.6573031
GGTATCA6700.022.3656711
GTACGAA502.7010456E-422.228
GCCGCAC555.141099E-420.18181836
CTGTACG609.2332624E-418.526
TGCGGTA701.21857294E-418.536
CGGATGG609.2332624E-418.527
CTTGCGG701.21857294E-418.534
TACGAAA609.2332624E-418.529
TTGCGGT701.21857294E-418.535
GTCCGCA701.21857294E-418.524
CCTTGCG701.21857294E-418.533
CCATCGG1351.1496013E-917.81481419
CGCGCGA752.0662154E-417.26666632
GCTTTAT3000.017.2666661
GCCGTTA1401.8662831E-917.17857215
CCGTTAA1401.8662831E-917.17857216
GTATAGA1708.54925E-1116.323531
ATCAACG24600.016.3191053
Produced by FastQC (version 0.11.5)