##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785296
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.553851031967564	33.0	33.0	33.0	27.0	33.0
2	31.736484077341537	33.0	33.0	33.0	27.0	33.0
3	31.458847364560626	33.0	33.0	33.0	27.0	33.0
4	31.76559666673458	33.0	33.0	33.0	27.0	33.0
5	31.87642239359426	33.0	33.0	33.0	33.0	33.0
6	34.937841272590205	37.0	37.0	37.0	27.0	37.0
7	35.09972545384161	37.0	37.0	37.0	33.0	37.0
8	35.234025131924774	37.0	37.0	37.0	33.0	37.0
9	35.35313945314888	37.0	37.0	37.0	33.0	37.0
10	35.35768550966769	37.0	37.0	37.0	33.0	37.0
11	35.36766900633646	37.0	37.0	37.0	33.0	37.0
12	35.37598943583057	37.0	37.0	37.0	33.0	37.0
13	35.3648280393635	37.0	37.0	37.0	33.0	37.0
14	35.34938036103584	37.0	37.0	37.0	33.0	37.0
15	35.35462042338175	37.0	37.0	37.0	33.0	37.0
16	35.32937771235305	37.0	37.0	37.0	33.0	37.0
17	35.33900465556936	37.0	37.0	37.0	33.0	37.0
18	35.32523914549418	37.0	37.0	37.0	33.0	37.0
19	35.319907397974774	37.0	37.0	37.0	33.0	37.0
20	35.32552311485096	37.0	37.0	37.0	33.0	37.0
21	35.32440761190685	37.0	37.0	37.0	33.0	37.0
22	35.22337691774821	37.0	37.0	37.0	33.0	37.0
23	35.26631359385505	37.0	37.0	37.0	33.0	37.0
24	35.25057430573949	37.0	37.0	37.0	33.0	37.0
25	35.271773955298386	37.0	37.0	37.0	33.0	37.0
26	35.15469453556366	37.0	37.0	37.0	33.0	37.0
27	35.16847150628553	37.0	37.0	37.0	33.0	37.0
28	35.18010024245635	37.0	37.0	37.0	33.0	37.0
29	35.187876927935456	37.0	37.0	37.0	33.0	37.0
30	35.18579109023859	37.0	37.0	37.0	33.0	37.0
31	35.18407326664086	37.0	37.0	37.0	33.0	37.0
32	35.173535838715594	37.0	37.0	37.0	33.0	37.0
33	35.17222805158819	37.0	37.0	37.0	33.0	37.0
34	35.14738264297794	37.0	37.0	37.0	33.0	37.0
35	35.08773889081314	37.0	37.0	37.0	33.0	37.0
36	35.11029981051731	37.0	37.0	37.0	33.0	37.0
37	35.10970131007926	37.0	37.0	37.0	33.0	37.0
38	35.09160876917748	37.0	37.0	37.0	33.0	37.0
39	34.99615940995497	37.0	37.0	37.0	27.0	37.0
40	34.77086729080499	37.0	37.0	37.0	27.0	37.0
41	34.954279660153624	37.0	37.0	37.0	27.0	37.0
42	35.00272381369573	37.0	37.0	37.0	27.0	37.0
43	34.632725749271614	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	31.0
16	57.0
17	37.0
18	28.0
19	47.0
20	96.0
21	237.0
22	624.0
23	1333.0
24	2623.0
25	4490.0
26	6897.0
27	9976.0
28	13692.0
29	18017.0
30	22623.0
31	28214.0
32	35888.0
33	46448.0
34	66724.0
35	131306.0
36	395903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67167030011613	21.244091399930724	14.08373403149895	24.000504268454186
2	15.789714960982865	22.121594914529044	39.053936350115116	23.034753774372977
3	17.687470711680692	27.841476335037996	30.432473869725555	24.038579083555756
4	12.235641083107517	18.41853262973452	40.68211222265235	28.663714064505612
5	13.456199955176137	37.35967074835476	36.0410596768607	13.143069619608402
6	28.43806666530837	41.05623866669384	17.617942788451742	12.887751879546055
7	25.704829771194554	32.73670055622338	24.251747111917034	17.306722560665023
8	24.143380330474116	35.95408610256515	21.198503494223832	18.704030072736906
9	25.651092072288666	15.82065870703531	20.972728754507855	37.55552046616817
10	14.510197428740245	28.68243312075956	34.60007436686294	22.207295083637252
11	33.04919418919745	24.451926407367413	24.7422627900817	17.756616613353433
12	22.575181842260754	26.311225321407473	30.922734866852753	20.190857969479026
13	27.58934210794401	22.78975061632811	26.6451121615289	22.975795114198977
14	21.53875736028198	21.584345265988876	28.669571728367394	28.20732564536175
15	23.25721766060186	29.556116419795845	25.032471832277253	22.154194087325035
16	22.23314520894032	29.23381756687924	25.84388561765245	22.689151606527986
17	21.74326623336933	28.256861107149405	27.163133391740185	22.836739267741084
18	22.422882581854488	27.835491330657486	28.595077524907808	21.146548562580225
19	24.22284081416434	26.864774556345633	28.318239237179355	20.59414539231067
20	23.83445223202461	26.903613414559608	28.14008985147002	21.121844501945763
21	22.61796825655549	27.4821213911697	28.235595240520773	21.66431511175404
22	22.076134349340887	27.502241193129723	28.441632199832927	21.97999225769646
23	22.134328966402478	27.18159776695666	28.65148937470712	22.032583891933744
24	22.03309325400868	27.75985106252929	27.925775758440132	22.281279925021902
25	22.429376948309937	27.642315763737496	28.2731606935474	21.655146594405167
26	22.439054827733745	27.572660499989816	28.20057659786883	21.78770807440761
27	22.314261119374095	27.501731831054787	27.965633340803976	22.21837370876714
28	22.135984393146025	27.327020639351275	28.531279925021902	22.005715042480798
29	22.962806381288075	27.518031417452782	27.92361096962165	21.595551231637497
30	22.848072573908436	27.256219310934988	28.011730608585804	21.88397750657077
31	22.269819278335813	27.40673580407897	28.350583728937877	21.97286118864734
32	21.618345184490945	27.307028177909988	28.52758704997861	22.547039587620464
33	21.56651759336607	27.456398606385363	28.809900980012632	22.167182820235936
34	22.640762209408933	26.92678938896925	28.442268902426598	21.990179499195207
35	22.283062692284183	27.61863042725291	27.945895560400153	22.152411320062754
36	22.757024103013386	27.498675658605165	27.93405289215786	21.81024734622359
37	22.570597583586316	26.961426010064994	28.107745359711494	22.360231046637193
38	22.576200566410627	27.274683686151462	27.998487194637434	22.150628552800473
39	22.24919011430085	27.34905054909232	28.247310568244334	22.154448768362503
40	22.107714797987	27.498038956011488	28.4845459546464	21.909700291355108
41	21.95388745135592	26.90348607404087	28.48098042012184	22.66164605448137
42	21.895056131700656	27.59290764246857	28.319385301847966	22.192650923982804
43	22.45204356064465	26.990714329373894	28.224643955909617	22.33259815407184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	42.5
2	53.0
3	202.0
4	351.0
5	351.0
6	438.5
7	526.0
8	504.5
9	483.0
10	625.0
11	767.0
12	767.0
13	1303.5
14	1840.0
15	3277.0
16	4714.0
17	5623.5
18	6533.0
19	6533.0
20	6789.5
21	7046.0
22	8305.5
23	9565.0
24	12276.0
25	14987.0
26	14987.0
27	18605.5
28	22224.0
29	26070.5
30	29917.0
31	33454.5
32	36992.0
33	36992.0
34	41987.5
35	46983.0
36	50123.0
37	53263.0
38	55631.0
39	57999.0
40	57999.0
41	59131.0
42	60263.0
43	60389.5
44	60516.0
45	59079.0
46	57642.0
47	57642.0
48	55517.5
49	53393.0
50	52211.5
51	51030.0
52	48745.5
53	46461.0
54	46461.0
55	44241.0
56	42021.0
57	36750.5
58	31480.0
59	29375.0
60	27270.0
61	27270.0
62	24188.0
63	21106.0
64	17765.5
65	14425.0
66	11975.5
67	9526.0
68	9526.0
69	8043.0
70	6560.0
71	5523.5
72	4487.0
73	3313.0
74	2139.0
75	2139.0
76	1622.0
77	1105.0
78	842.0
79	579.0
80	485.5
81	392.0
82	392.0
83	324.5
84	257.0
85	225.0
86	193.0
87	147.5
88	102.0
89	102.0
90	76.0
91	50.0
92	35.0
93	20.0
94	12.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	785296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.69832915890154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19990876470489	58.60284966967569
2	6.558130993240824	8.617164973222593
3	1.4654673706879215	2.8883627307325517
4	0.6620526469014917	1.739830108666249
5	0.39739292817719896	1.305402570040267
6	0.2692605363649341	1.0613980408563493
7	0.1920412182812806	0.8831751019504024
8	0.1424604087007461	0.7487528658346609
9	0.12447122113041734	0.7359796130972922
>10	0.8293769483233725	10.814539034436036
>50	0.100873030565251	4.525644613015991
>100	0.05640940696341897	6.521279854536739
>500	0.0015669279696762298	0.6533118473917966
>1k	5.875979886285861E-4	0.902308976543486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3368	0.42888286709724743	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2323	0.2958120250198651	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.1682168252480593	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	880	0.11205965648621666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.2734051873433713E-4	0.0	0.0
2	0.0	0.0	1.2734051873433713E-4	0.0	0.0
3	0.0	0.0	1.2734051873433713E-4	0.0	0.0
4	0.0	0.0	2.5468103746867425E-4	1.2734051873433713E-4	0.0
5	0.0	0.0	3.8202155620301135E-4	1.2734051873433713E-4	0.0
6	0.0	0.0	7.640431124060227E-4	2.5468103746867425E-4	0.0
7	0.0	0.0	0.002546810374686742	2.5468103746867425E-4	0.0
8	0.0	0.0	0.0029288319308897535	2.5468103746867425E-4	0.0
9	0.0	0.0	0.003056172449624091	3.8202155620301135E-4	0.0
10	1.2734051873433713E-4	0.0	0.0036928750432957762	3.8202155620301135E-4	0.0
11	1.2734051873433713E-4	0.0	0.005475642305576496	5.093620749373485E-4	0.0
12	1.2734051873433713E-4	0.0	0.00573032334304517	5.093620749373485E-4	0.0
13	1.2734051873433713E-4	0.0	0.006112344899248182	5.093620749373485E-4	0.0
14	1.2734051873433713E-4	0.0	0.006239685417982518	5.093620749373485E-4	0.0
15	1.2734051873433713E-4	0.0	0.006876388011654204	5.093620749373485E-4	0.0
16	1.2734051873433713E-4	0.0	0.00751309060532589	5.093620749373485E-4	0.0
17	1.2734051873433713E-4	0.0	0.007640431124060227	6.367025936716855E-4	0.0
18	1.2734051873433713E-4	0.0	0.0078951121615289	6.367025936716855E-4	0.0
19	1.2734051873433713E-4	0.0	0.008022452680263237	6.367025936716855E-4	0.0
20	3.8202155620301135E-4	0.0	0.008022452680263237	8.913836311403599E-4	0.0
21	3.8202155620301135E-4	0.0	0.00840447423646625	0.0014007457060777084	0.0
22	3.8202155620301135E-4	0.0	0.00840447423646625	0.0017827672622807197	0.0
23	3.8202155620301135E-4	0.0	0.00840447423646625	0.002164788818483731	0.0
24	3.8202155620301135E-4	0.0	0.008531814755200587	0.002292129337218068	0.0
25	3.8202155620301135E-4	0.0	0.008531814755200587	0.0026741508934210794	0.0
26	3.8202155620301135E-4	0.0	0.008531814755200587	0.0028014914121554167	0.0
27	3.8202155620301135E-4	0.0	0.008531814755200587	0.0038202155620301135	0.0
28	3.8202155620301135E-4	0.0	0.008531814755200587	0.01324341394837106	0.0
29	3.8202155620301135E-4	0.0	0.008531814755200587	0.027632892565351154	0.0
30	3.8202155620301135E-4	0.0	0.008531814755200587	0.045333224669424015	0.0
31	5.093620749373485E-4	0.0	0.008531814755200587	0.09474134593834681	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACGA	45	1.3226905E-4	24.666668	27
GTATCAA	1780	0.0	22.657303	1
GGTATCA	670	0.0	22.365671	1
GTACGAA	50	2.7010456E-4	22.2	28
GCCGCAC	55	5.141099E-4	20.181818	36
CTGTACG	60	9.2332624E-4	18.5	26
TGCGGTA	70	1.21857294E-4	18.5	36
CGGATGG	60	9.2332624E-4	18.5	27
CTTGCGG	70	1.21857294E-4	18.5	34
TACGAAA	60	9.2332624E-4	18.5	29
TTGCGGT	70	1.21857294E-4	18.5	35
GTCCGCA	70	1.21857294E-4	18.5	24
CCTTGCG	70	1.21857294E-4	18.5	33
CCATCGG	135	1.1496013E-9	17.814814	19
CGCGCGA	75	2.0662154E-4	17.266666	32
GCTTTAT	300	0.0	17.266666	1
GCCGTTA	140	1.8662831E-9	17.178572	15
CCGTTAA	140	1.8662831E-9	17.178572	16
GTATAGA	170	8.54925E-11	16.32353	1
ATCAACG	2460	0.0	16.319105	3
>>END_MODULE