FastQCFastQC Report
Fri 10 Feb 2017
ERR1633265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762847
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT222302.9140836891277018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT166002.1760588951650854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92591.2137427295381642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.5462432178405369No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA37080.4860738785103697No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG32340.423938220901439No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31180.4087320262123335No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT29320.3843496795556645No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28770.3771398458668645No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC25950.34017306222610827No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA25100.32903059197978096No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA23840.3125135184381665No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.30884305765114106No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG21400.2805280744369447No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19310.2531307064195048No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19120.25064003659973755No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18120.2375312480746467No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA17280.22651986571357036No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC17240.22599551417256675No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC17200.22547116263156308No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16510.21642609854925038No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16200.2123623741064722No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16020.21000279217195583No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC15440.20239969482740314No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14960.19610747633535952No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA14880.19505877325335225No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14480.1898152578433159No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14390.1886354668760577No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC14320.18771785167930136No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA14240.18666914859729408No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14230.18653806071204315No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.176706469318225No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13470.1765753814329741No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13430.17605102989197047No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13160.17251165699019594No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12940.16962772351467595No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12710.16661270215390503No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12590.16503964753089415No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12530.16425312021938868No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12300.16123809885861778No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12170.15953395635035597No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11660.15284847420255962No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC11640.1525862984320578No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11620.15232412266155598No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11580.15179977112055237No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11570.15166868323530144No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11490.15061998015329417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.1493091013007851No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11360.14891583764503236No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11280.14786713456302508No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11260.14760495879252328No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11210.14694951936626874No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11180.146556255710516No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11040.1447210253170033No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG11020.14445884954650146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.142230355497236No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10570.13855989471021057No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC10570.13855989471021057No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10490.1375111916282033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10440.13685575220194876No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10400.1363314006609451No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10310.13515160969368695No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10310.13515160969368695No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10290.13488943392318511No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10060.13187441256241422No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10010.13121897313615968No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9960.13056353370990512No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9870.12938374274264697No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9860.12925265485739604No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9830.12885939120164333No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9710.12728633657863242No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG9700.12715524869338152No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9670.12676198503762878No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA9650.12649980926712695No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9550.12518893041461787No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9510.12466457887361423No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9490.12440240310311242No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9400.12322261213585425No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG9390.12309152425060332No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG9290.12178064539809423No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9270.12151846962759243No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9120.11955215134882878No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8980.11771692095531607No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8770.11496407536504699No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT8720.11430863593879244No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG8650.11339102074203608No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8640.11325993285678518No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8440.110638175151767No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8410.11024491149601427No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8390.10998273572551247No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA8210.10762315379099609No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8120.10644336282373792No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG8020.10513248397122883No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.10395269300397066No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7800.10224855049570883No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7790.10211746261045793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACA200.001841548137.05
TGTACTA200.001841548137.05
TTCTCGC200.001841548137.010
GTGACAT601.2696546E-930.83333416
TGTCAAC502.7187525E-729.5999982
TTATGAC250.005495199529.5999983
TAACAGT250.005495199529.5999987
CTTCTCG250.005495199529.5999989
CGGTCGC250.005495199529.59999821
GTAAACA250.005495199529.59999815
GCCTAGT405.9375514E-527.751
TAGTTCC509.085856E-625.8999984
GACATCT809.786163E-1025.437518
CCTAGTT451.3226426E-424.6666662
GAAACTT451.3226426E-424.6666665
GGTATCA35200.023.9133531
GACCGTT1400.023.7857137
GGACCCA701.921162E-723.78571334
TTCCCTG551.901571E-523.54545436
CTAGTTC551.901571E-523.5454543
Produced by FastQC (version 0.11.5)