FastQCFastQC Report
Fri 10 Feb 2017
ERR1633264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856130
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46450.5425577891208111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.3959679020709471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19520.22800275659070468No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9290.1085115578241622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.027.068421
TCGCCAT1300.024.19230813
GTATCAA24350.022.4887051
ACGTGAT705.099413E-621.14285937
CTCGCTA450.003825022420.55555523
GGACCGT555.141619E-420.1818186
GCCGCTC851.2445489E-619.58823627
CCGTCCG851.2445489E-619.58823628
TTCGCCG851.2445489E-619.58823624
CGAGTCG951.6748709E-719.47368421
GAGTCGC951.6748709E-719.47368422
TTGTCCG1153.0468073E-919.30434813
TCTATAC1457.2759576E-1219.137933
GTCGCCA1650.019.06060612
TCGCACG609.234189E-418.522
AGCTTCG1002.8727845E-718.521
TGCGGGT801.615885E-518.521
ACGATTA801.615885E-518.523
GCTTCGC1002.8727845E-718.522
AACGATT801.615885E-518.522
Produced by FastQC (version 0.11.5)