##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633264.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	856130
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775902024225292	33.0	33.0	33.0	27.0	33.0
2	31.910209898029503	33.0	33.0	33.0	33.0	33.0
3	31.642325347785967	33.0	33.0	33.0	27.0	33.0
4	31.92490743228248	33.0	33.0	33.0	33.0	33.0
5	32.0333874528401	33.0	33.0	33.0	33.0	33.0
6	35.180866223587536	37.0	37.0	37.0	33.0	37.0
7	35.34037704554215	37.0	37.0	37.0	33.0	37.0
8	35.47191548012568	37.0	37.0	37.0	33.0	37.0
9	35.58941399086587	37.0	37.0	37.0	33.0	37.0
10	35.59715580577716	37.0	37.0	37.0	33.0	37.0
11	35.61474308808242	37.0	37.0	37.0	33.0	37.0
12	35.6146473082359	37.0	37.0	37.0	33.0	37.0
13	35.6098886851296	37.0	37.0	37.0	33.0	37.0
14	35.592120355553476	37.0	37.0	37.0	33.0	37.0
15	35.59652973263406	37.0	37.0	37.0	33.0	37.0
16	35.58203660659012	37.0	37.0	37.0	33.0	37.0
17	35.58558162895822	37.0	37.0	37.0	33.0	37.0
18	35.5669466085758	37.0	37.0	37.0	33.0	37.0
19	35.5587364068541	37.0	37.0	37.0	33.0	37.0
20	35.54894934180557	37.0	37.0	37.0	33.0	37.0
21	35.55432352563279	37.0	37.0	37.0	33.0	37.0
22	35.460771144569165	37.0	37.0	37.0	33.0	37.0
23	35.509760199969634	37.0	37.0	37.0	33.0	37.0
24	35.49728779507785	37.0	37.0	37.0	33.0	37.0
25	35.52058799481387	37.0	37.0	37.0	33.0	37.0
26	35.409217058157054	37.0	37.0	37.0	33.0	37.0
27	35.42550313620595	37.0	37.0	37.0	33.0	37.0
28	35.44436709378249	37.0	37.0	37.0	33.0	37.0
29	35.44056276500064	37.0	37.0	37.0	33.0	37.0
30	35.454462523214936	37.0	37.0	37.0	33.0	37.0
31	35.44529802716877	37.0	37.0	37.0	33.0	37.0
32	35.42611285669233	37.0	37.0	37.0	33.0	37.0
33	35.42550313620595	37.0	37.0	37.0	33.0	37.0
34	35.403702708700784	37.0	37.0	37.0	33.0	37.0
35	35.344252625185426	37.0	37.0	37.0	33.0	37.0
36	35.36971955193721	37.0	37.0	37.0	33.0	37.0
37	35.36297408103909	37.0	37.0	37.0	33.0	37.0
38	35.34698351885812	37.0	37.0	37.0	33.0	37.0
39	35.246249985399416	37.0	37.0	37.0	33.0	37.0
40	35.03744524780115	37.0	37.0	37.0	27.0	37.0
41	35.20215271045285	37.0	37.0	37.0	33.0	37.0
42	35.24252274771355	37.0	37.0	37.0	33.0	37.0
43	34.89731115601602	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	22.0
16	47.0
17	33.0
18	23.0
19	38.0
20	70.0
21	166.0
22	435.0
23	978.0
24	2033.0
25	3681.0
26	5923.0
27	8896.0
28	12190.0
29	16376.0
30	21431.0
31	27506.0
32	35035.0
33	45755.0
34	66461.0
35	133877.0
36	475146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.218740144604205	21.016901638769813	14.221788747036083	23.5425694695899
2	15.596346349269385	22.503708548935325	39.79851190823823	22.101433193557053
3	17.632368915935665	27.812364944576174	31.243035520306496	23.31223061918167
4	12.304673355681963	18.31731162323479	40.29014285213694	29.087872168946305
5	13.206288764556785	37.70630628526042	35.73803043930244	13.349374510880358
6	28.166166353240747	40.828145258313576	17.757116325791642	13.248572062654034
7	25.16708911029867	33.2329202340766	24.011423498767712	17.588567156857017
8	23.895553245418334	36.343896370878255	21.110579000852674	18.649971382850737
9	25.595995935196758	15.902491444056393	20.870662165792577	37.63085045495427
10	14.487986637543363	28.655811617394555	34.41171317440108	22.444488570660997
11	32.58734070760282	24.551061170616613	24.78315209138799	18.07844603039258
12	22.185766180369804	26.585331666919743	30.993190286521905	20.235711866188545
13	27.556796280938645	23.036337939331645	27.03888428159275	22.367981498136967
14	21.790148692371485	22.007755831474192	28.743064721479218	27.459030754675105
15	23.409528926681695	29.23948465770385	25.54810601193744	21.802880403677012
16	22.01044233936435	29.51269082966372	25.846425192435728	22.630441638536205
17	21.769941480849873	28.155069907607487	27.096819408267436	22.978169203275204
18	22.18494854753367	27.587866328711762	28.738275729153283	21.48890939460129
19	24.098559798161496	26.736126522841158	28.91955660939343	20.245757069603915
20	23.885624846693844	26.45637928819221	28.831953091236144	20.8260427738778
21	22.18296286778877	27.747538341139784	28.19747001039562	21.87202878067583
22	21.71702895588287	27.709343207223203	28.447315244180203	22.126312592713724
23	21.871795171294078	27.336385829254905	28.922943945428848	21.86887505402217
24	22.38211486573301	27.640662048987885	28.067115975377572	21.91010710990153
25	22.450328805204816	27.26361650683892	28.714797986287127	21.57125670166914
26	22.36295889642928	27.334516954200883	28.435985189165198	21.866538960204643
27	22.260637987221568	27.26361650683892	28.21826124537161	22.257484260567907
28	21.64461004753951	27.562986929555088	28.840947052433624	21.951455970471777
29	22.74327496992279	27.402614088981814	28.083935850863774	21.770175090231625
30	22.07667059909126	27.65316015091166	28.63852452314485	21.63164472685223
31	22.504642986462336	27.392568885566444	28.4448623456718	21.65792578229942
32	21.43319355705325	27.33089600878371	28.696926868583045	22.538983565579993
33	21.604429233878033	27.22577178699497	29.27873103383832	21.891067945288682
34	21.870393515003563	27.23523296695595	28.642612687325524	22.25176083071496
35	22.01850186303482	27.843785406421922	28.25622276990644	21.88148996063682
36	22.445189398806257	27.61052643874178	28.189293682034272	21.754990480417693
37	22.696903507644866	26.919509887517084	27.9848854730006	22.398701131837452
38	22.485720626540363	27.503650146589887	28.139768493102686	21.870860733767067
39	22.129349514676512	27.210703981871916	28.458528494504336	22.201418008947236
40	22.046301379463397	27.53845794447105	28.592853889012183	21.822386787053368
41	21.856493756789273	27.117610643243435	28.785815238339975	22.240080361627324
42	21.863618842932734	27.43637064464509	28.44112459556376	22.25888591685842
43	21.989183885624847	27.039701914428882	28.492635464240244	22.478478735706027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	48.5
2	64.0
3	274.0
4	484.0
5	484.0
6	574.5
7	665.0
8	649.0
9	633.0
10	913.5
11	1194.0
12	1194.0
13	1811.5
14	2429.0
15	4761.0
16	7093.0
17	8058.5
18	9024.0
19	9024.0
20	8834.5
21	8645.0
22	9965.0
23	11285.0
24	14079.5
25	16874.0
26	16874.0
27	20252.5
28	23631.0
29	28954.0
30	34277.0
31	37313.5
32	40350.0
33	40350.0
34	44652.0
35	48954.0
36	51672.0
37	54390.0
38	57311.5
39	60233.0
40	60233.0
41	62724.5
42	65216.0
43	63272.5
44	61329.0
45	61510.5
46	61692.0
47	61692.0
48	60089.0
49	58486.0
50	59312.0
51	60138.0
52	59572.0
53	59006.0
54	59006.0
55	53378.0
56	47750.0
57	42234.0
58	36718.0
59	32237.5
60	27757.0
61	27757.0
62	24179.0
63	20601.0
64	16832.5
65	13064.0
66	10924.0
67	8784.0
68	8784.0
69	7208.0
70	5632.0
71	4874.5
72	4117.0
73	3294.0
74	2471.0
75	2471.0
76	1850.5
77	1230.0
78	934.5
79	639.0
80	530.5
81	422.0
82	422.0
83	367.0
84	312.0
85	281.0
86	250.0
87	195.5
88	141.0
89	141.0
90	111.5
91	82.0
92	56.5
93	31.0
94	17.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	856130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.52550807790114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96056476175032	52.35897308262314
2	7.254557102391102	8.63662394799953
3	1.6547231225350536	2.9549470379145037
4	0.7081939559300915	1.6862242017774969
5	0.44659231993050086	1.3291817373775818
6	0.29119641929952467	1.0400168885561845
7	0.2032336121022841	0.8468308813226876
8	0.16186817132518042	0.7708228111817689
9	0.13088560008555952	0.701192866065652
>10	0.9481233609855179	11.544043689627284
>50	0.14661718537042664	6.130811154074771
>100	0.08967486944938952	9.614791178302537
>500	0.0031743316589387125	1.2061151169903963
>1k	5.951871860510085E-4	1.1794254061865355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4645	0.5425577891208111	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3390	0.3959679020709471	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1952	0.22800275659070468	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	929	0.1085115578241622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.1680469087638559E-4	0.0	0.0
2	0.0	0.0	1.1680469087638559E-4	0.0	0.0
3	0.0	0.0	2.3360938175277118E-4	0.0	0.0
4	0.0	0.0	2.3360938175277118E-4	0.0	0.0
5	0.0	0.0	4.6721876350554236E-4	0.0	0.0
6	0.0	0.0	9.344375270110847E-4	0.0	0.0
7	0.0	0.0	0.002803312581033254	1.1680469087638559E-4	0.0
8	0.0	0.0	0.0036209454171679534	1.1680469087638559E-4	0.0
9	0.0	0.0	0.003971359489797111	4.6721876350554236E-4	0.0
10	0.0	0.0	0.004555382944179038	4.6721876350554236E-4	0.0
11	1.1680469087638559E-4	0.0	0.006307453307324822	4.6721876350554236E-4	0.0
12	1.1680469087638559E-4	0.0	0.007125086143459521	4.6721876350554236E-4	0.0
13	1.1680469087638559E-4	0.0	0.007592304906965064	4.6721876350554236E-4	0.0
14	1.1680469087638559E-4	0.0	0.007825914288717834	4.6721876350554236E-4	0.0
15	1.1680469087638559E-4	0.0	0.007942718979594221	5.84023454381928E-4	0.0
16	1.1680469087638559E-4	0.0	0.00899396119748169	7.008281452583135E-4	0.0
17	1.1680469087638559E-4	0.0	0.009227570579234462	8.176328361346991E-4	0.0
18	1.1680469087638559E-4	0.0	0.009577984651863618	8.176328361346991E-4	0.0
19	1.1680469087638559E-4	0.0	0.010045203415369162	0.0010512422178874704	0.0
20	1.1680469087638559E-4	0.0	0.010162008106245547	0.001168046908763856	0.0
21	1.1680469087638559E-4	0.0	0.010162008106245547	0.001401656290516627	0.0
22	1.1680469087638559E-4	0.0	0.010162008106245547	0.0019856797448985553	0.0
23	1.1680469087638559E-4	0.0	0.010162008106245547	0.0022192891266513263	0.0
24	1.1680469087638559E-4	0.0	0.010162008106245547	0.002569703199280483	0.0
25	2.3360938175277118E-4	0.0	0.010162008106245547	0.002803312581033254	0.0
26	2.3360938175277118E-4	0.0	0.010162008106245547	0.003737750108044339	0.0
27	3.5041407262915677E-4	0.0	0.010162008106245547	0.004204968871549882	0.0
28	3.5041407262915677E-4	0.0	0.010162008106245547	0.011213250324133017	0.0
29	3.5041407262915677E-4	0.0	0.010162008106245547	0.02814993050120893	0.0
30	3.5041407262915677E-4	0.0	0.010162008106245547	0.04812353264107087	0.0
31	3.5041407262915677E-4	0.0	0.010162008106245547	0.1071099015336456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	950	0.0	27.06842	1
TCGCCAT	130	0.0	24.192308	13
GTATCAA	2435	0.0	22.488705	1
ACGTGAT	70	5.099413E-6	21.142859	37
CTCGCTA	45	0.0038250224	20.555555	23
GGACCGT	55	5.141619E-4	20.181818	6
GCCGCTC	85	1.2445489E-6	19.588236	27
CCGTCCG	85	1.2445489E-6	19.588236	28
TTCGCCG	85	1.2445489E-6	19.588236	24
CGAGTCG	95	1.6748709E-7	19.473684	21
GAGTCGC	95	1.6748709E-7	19.473684	22
TTGTCCG	115	3.0468073E-9	19.304348	13
TCTATAC	145	7.2759576E-12	19.13793	3
GTCGCCA	165	0.0	19.060606	12
TCGCACG	60	9.234189E-4	18.5	22
AGCTTCG	100	2.8727845E-7	18.5	21
TGCGGGT	80	1.615885E-5	18.5	21
ACGATTA	80	1.615885E-5	18.5	23
GCTTCGC	100	2.8727845E-7	18.5	22
AACGATT	80	1.615885E-5	18.5	22
>>END_MODULE