FastQCFastQC Report
Fri 10 Feb 2017
ERR1633263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288256
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11670.4048484680284192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.32054840142095914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6270.217514986678508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG250.00548959829.610
GGTATCA2900.028.7068961
CGTTAAA358.8532135E-426.42857217
GGCCGCA451.319855E-424.66666633
GGCCGTT400.001927698123.12514
ACGGGTG400.001927698123.1255
GCCGTTA400.001927698123.12515
CCGTTAA400.001927698123.12516
TTGGCCG502.6953017E-422.231
GTATCAA6800.021.2205891
CCGTCCG450.003818961620.55555528
CCTGGCG450.003818961620.55555533
CGCCGTC450.003818961620.55555526
CGTCCGC450.003818961620.55555529
TCGCCAT555.1302573E-420.18181813
GTCGCCA555.1302573E-420.18181812
GCAGGCG555.1302573E-420.18181837
TCTATAC656.879226E-519.9230773
TCGCCGT500.007022412518.525
GGTACCG500.007022412518.56
Produced by FastQC (version 0.11.5)