FastQCFastQC Report
Fri 10 Feb 2017
ERR1633262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807332
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38810.4807192084545143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27220.3371599292484381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16610.20573939841353994No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11090.13736604024118948No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10000.12386477929773625No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9200.11395559695391735No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8690.1076384932097328No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8270.10243617247922789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.025.0569611
GTATCAA19800.022.611111
TATACTG1350.021.9259245
GCACCGC2550.021.76470810
TTAACGG2550.021.76470835
AACGGCC2650.020.94339637
CGTGGAT450.00382483620.55555712
TAACGGC2750.019.5090936
TATTAGA3150.019.3809532
ATTAGAG3250.019.3538463
CACCGCC2800.019.16071311
GGTCGCC1850.019.036
AGGTCGC1850.019.035
GTCGCCC1950.018.02564237
GTATTAG3550.017.7183111
TCTTACC2250.017.2666662
GTGACAC3000.017.26666624
TCTAGAC650.001579383717.0769233
TTAGAGG4250.016.976474
ACGAGGG2850.016.87719315
Produced by FastQC (version 0.11.5)