##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633262.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	807332
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889220295987275	33.0	33.0	33.0	33.0	33.0
2	32.00377292117741	33.0	33.0	33.0	33.0	33.0
3	31.717714645276047	33.0	33.0	33.0	27.0	33.0
4	32.00222585008398	33.0	33.0	33.0	33.0	33.0
5	32.10333295348134	33.0	33.0	33.0	33.0	33.0
6	35.28728082127303	37.0	37.0	37.0	33.0	37.0
7	35.42443505274162	37.0	37.0	37.0	33.0	37.0
8	35.55753147404042	37.0	37.0	37.0	33.0	37.0
9	35.6655502321226	37.0	37.0	37.0	33.0	37.0
10	35.67927321102099	37.0	37.0	37.0	33.0	37.0
11	35.69957960293906	37.0	37.0	37.0	33.0	37.0
12	35.690698498263416	37.0	37.0	37.0	33.0	37.0
13	35.68948957801747	37.0	37.0	37.0	33.0	37.0
14	35.667389624095165	37.0	37.0	37.0	33.0	37.0
15	35.68744085456788	37.0	37.0	37.0	33.0	37.0
16	35.66067863035282	37.0	37.0	37.0	33.0	37.0
17	35.66421373115397	37.0	37.0	37.0	33.0	37.0
18	35.64099280097903	37.0	37.0	37.0	33.0	37.0
19	35.63224794756061	37.0	37.0	37.0	33.0	37.0
20	35.63688049030634	37.0	37.0	37.0	33.0	37.0
21	35.63411335113683	37.0	37.0	37.0	33.0	37.0
22	35.55067679715408	37.0	37.0	37.0	33.0	37.0
23	35.600605451041204	37.0	37.0	37.0	33.0	37.0
24	35.58241838549692	37.0	37.0	37.0	33.0	37.0
25	35.60677763299361	37.0	37.0	37.0	33.0	37.0
26	35.50260363766084	37.0	37.0	37.0	33.0	37.0
27	35.51911481274123	37.0	37.0	37.0	33.0	37.0
28	35.54257480194022	37.0	37.0	37.0	33.0	37.0
29	35.54587455966071	37.0	37.0	37.0	33.0	37.0
30	35.54521683768264	37.0	37.0	37.0	33.0	37.0
31	35.53166355353188	37.0	37.0	37.0	33.0	37.0
32	35.51674404086547	37.0	37.0	37.0	33.0	37.0
33	35.519617703745176	37.0	37.0	37.0	33.0	37.0
34	35.50500042114025	37.0	37.0	37.0	33.0	37.0
35	35.44181947451606	37.0	37.0	37.0	33.0	37.0
36	35.460654352856075	37.0	37.0	37.0	33.0	37.0
37	35.466827773456274	37.0	37.0	37.0	33.0	37.0
38	35.450599010072686	37.0	37.0	37.0	33.0	37.0
39	35.352999757225035	37.0	37.0	37.0	33.0	37.0
40	35.143984135399066	37.0	37.0	37.0	33.0	37.0
41	35.314596721051565	37.0	37.0	37.0	33.0	37.0
42	35.349551361769386	37.0	37.0	37.0	33.0	37.0
43	35.02951945420224	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	27.0
16	45.0
17	31.0
18	29.0
19	21.0
20	40.0
21	120.0
22	351.0
23	774.0
24	1607.0
25	2916.0
26	4995.0
27	7369.0
28	10663.0
29	14508.0
30	19075.0
31	24561.0
32	31349.0
33	41550.0
34	60615.0
35	124708.0
36	461973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04494805111156	20.798011227103597	13.82851169035787	24.32852903142697
2	15.823849420065104	21.62134041509565	39.303161524626795	23.25164864021245
3	17.62372852804051	27.615157085313108	30.01478945464815	24.746324931998238
4	12.153983739031773	18.05143361095559	40.15138753325769	29.64319511675494
5	13.396471340167365	37.45621380051825	35.62957990021454	13.517734959099851
6	28.855043526083445	40.52174322335792	17.44759281187913	13.175620438679502
7	26.17919269891445	32.42990492139541	23.931046954660538	17.459855425029602
8	24.169734384367274	36.23837529046291	20.547556643363574	19.044333681806247
9	25.803386958525117	15.549612798699917	20.504203970609368	38.142796272165604
10	14.915425128695507	28.092903539064473	34.04138570005896	22.95028563218106
11	33.56562108277635	23.93785951752191	24.396159200923538	18.100360198778198
12	22.850450620067086	26.217590780496746	30.271685006911653	20.660273592524515
13	27.689104358553855	22.787031853066644	26.199382657939978	23.324481130439523
14	21.735915335946054	21.36630283452161	28.080517061134703	28.81726476839764
15	23.8017321250737	28.84550593807752	24.829190469348422	22.523571467500357
16	22.566676410695973	28.821228441335162	25.41791976535056	23.194175382618305
17	22.153835101296618	27.779897241779096	26.62411498615192	23.44215267077237
18	22.780838614101757	27.497609409759555	28.034810957573836	21.686741018564852
19	24.54492080086012	26.50136498986786	27.84108644275218	21.112627766519847
20	23.676009374086497	26.385055962107284	28.148642689748453	21.79029197405776
21	22.594669850817258	27.187575867177323	28.064662369384592	22.15309191262083
22	22.47551193313284	26.98121714486729	28.036297334925408	22.506973587074462
23	22.389178181962315	26.951985056953028	28.453102317262292	22.205734443822365
24	22.592564149569196	27.641168688965628	26.935015582189237	22.831251579275936
25	22.641243007833207	27.17816214395069	28.05970777821268	22.12088707000342
26	22.670475095747474	27.396907344190495	27.96680919373938	21.965808366322655
27	22.59305960868639	27.210738580905996	27.465776161480036	22.73042564892758
28	22.171300035177595	26.991497921549	28.328989808405957	22.50821223486744
29	23.730262147418905	26.89711295972413	27.329648769031824	22.04297612382514
30	23.1397987445066	26.68418940411132	27.95256474412014	22.223447107261947
31	22.837197088682228	26.886460588704526	27.828947694380997	22.44739462823225
32	21.749788191227402	26.720110190107665	28.329485267523154	23.200616351141786
33	21.82869005564006	26.941580415492016	28.609791263073923	22.619938265793998
34	22.800533114010097	26.679358677718707	28.23708214216704	22.283026066104156
35	22.539178429691876	27.46515683758355	27.667304157397453	22.328360575327128
36	23.45268117701268	27.057270119356104	27.36296839466291	22.127080308968306
37	22.595041445155154	26.63105141379259	27.694926003180846	23.07898113787141
38	22.831251579275936	27.056031471563124	27.487824092195034	22.624892856965907
39	22.266428185678258	27.016147012629254	27.858055917515966	22.859368884176522
40	22.45903791748624	27.52523125554295	28.180599802807272	21.83513102416354
41	22.39983055298192	26.567384917233554	27.996165146432944	23.036619383351585
42	21.98909494483063	27.512597048054584	27.932498649873907	22.565809357240887
43	22.858873425059333	26.29847448137817	27.76193684878092	23.080715244781576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	37.0
2	52.0
3	226.0
4	400.0
5	400.0
6	486.0
7	572.0
8	530.0
9	488.0
10	647.0
11	806.0
12	806.0
13	1401.5
14	1997.0
15	3523.5
16	5050.0
17	5911.5
18	6773.0
19	6773.0
20	6452.5
21	6132.0
22	6580.0
23	7028.0
24	9259.0
25	11490.0
26	11490.0
27	14748.0
28	18006.0
29	21293.5
30	24581.0
31	28855.0
32	33129.0
33	33129.0
34	39636.0
35	46143.0
36	49475.5
37	52808.0
38	56088.0
39	59368.0
40	59368.0
41	61600.0
42	63832.0
43	64825.0
44	65818.0
45	64504.5
46	63191.0
47	63191.0
48	60240.0
49	57289.0
50	57998.5
51	58708.0
52	54707.0
53	50706.0
54	50706.0
55	48917.0
56	47128.0
57	40790.5
58	34453.0
59	31698.0
60	28943.0
61	28943.0
62	25335.5
63	21728.0
64	17992.0
65	14256.0
66	12095.5
67	9935.0
68	9935.0
69	8267.5
70	6600.0
71	5378.0
72	4156.0
73	3336.0
74	2516.0
75	2516.0
76	1913.0
77	1310.0
78	1035.5
79	761.0
80	614.5
81	468.0
82	468.0
83	393.0
84	318.0
85	260.5
86	203.0
87	152.0
88	101.0
89	101.0
90	79.5
91	58.0
92	31.5
93	5.0
94	4.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	807332.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.853446697043864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93103422133977	52.90053057307196
2	7.8219588312828074	9.519863096118799
3	2.020996557599617	3.689538188783923
4	0.8666719889385271	2.109599107307666
5	0.4881034757946349	1.485138942345533
6	0.31142860327013816	1.1370902345420506
7	0.22400934074934845	0.954221833385129
8	0.1684524277647545	0.8200728667176096
9	0.13638275267557756	0.7469424513305438
>10	0.843634352289117	10.014558966284875
>50	0.10548802437288789	4.468726756356038
>100	0.07484811635486914	8.540311114512622
>500	0.006168800795233982	2.4405536611739476
>1k	8.225067726978642E-4	1.1728522080692887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3881	0.4807192084545143	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2722	0.3371599292484381	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1661	0.20573939841353994	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1109	0.13736604024118948	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1000	0.12386477929773625	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	920	0.11395559695391735	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	869	0.1076384932097328	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	827	0.10243617247922789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.2386477929773624E-4	0.0	0.0
2	0.0	0.0	1.2386477929773624E-4	0.0	0.0
3	0.0	0.0	2.477295585954725E-4	0.0	0.0
4	0.0	0.0	6.193238964886812E-4	2.477295585954725E-4	0.0
5	0.0	0.0	6.193238964886812E-4	2.477295585954725E-4	0.0
6	0.0	0.0	0.0016102421308705711	2.477295585954725E-4	0.0
7	0.0	0.0	0.0037159433789320873	2.477295585954725E-4	0.0
8	0.0	0.0	0.004087537716825296	2.477295585954725E-4	0.0
9	0.0	0.0	0.004335267275420769	4.95459117190945E-4	0.0
10	3.7159433789320874E-4	0.0	0.00495459117190945	4.95459117190945E-4	0.0
11	4.95459117190945E-4	0.0	0.00693642764067323	6.193238964886812E-4	0.0
12	4.95459117190945E-4	0.0	0.007679616316459647	6.193238964886812E-4	0.0
13	4.95459117190945E-4	0.0	0.008670534550841538	6.193238964886812E-4	0.0
14	4.95459117190945E-4	0.0	0.009413723226627955	6.193238964886812E-4	0.0
15	4.95459117190945E-4	0.0	0.010280776681712109	8.670534550841537E-4	0.0
16	4.95459117190945E-4	0.0	0.010776235798903053	8.670534550841537E-4	0.0
17	4.95459117190945E-4	0.0	0.011271694916093998	9.9091823438189E-4	0.0
18	4.95459117190945E-4	0.0	0.011271694916093998	0.0011147830136796263	0.0
19	4.95459117190945E-4	0.0	0.011767154033284943	0.0011147830136796263	0.0
20	4.95459117190945E-4	0.0	0.012014883591880417	0.0013625125722750988	0.0
21	4.95459117190945E-4	0.0	0.012014883591880417	0.0018579716894660436	0.0
22	4.95459117190945E-4	0.0	0.012262613150475889	0.0023534308066569887	0.0
23	4.95459117190945E-4	0.0	0.012386477929773624	0.0027250251445501976	0.0
24	4.95459117190945E-4	0.0	0.012510342709071361	0.003344349041038879	0.0
25	4.95459117190945E-4	0.0	0.012510342709071361	0.003468213820336615	0.0
26	4.95459117190945E-4	0.0	0.012634207488369097	0.003592078599634351	0.0
27	4.95459117190945E-4	0.0	0.012634207488369097	0.00495459117190945	0.0
28	4.95459117190945E-4	0.0	0.012634207488369097	0.012758072267666834	0.0
29	4.95459117190945E-4	0.0	0.012634207488369097	0.030842330045136326	0.0
30	4.95459117190945E-4	0.0	0.012634207488369097	0.051032289070667335	0.0
31	4.95459117190945E-4	0.0	0.012634207488369097	0.10256003725852561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	25.056961	1
GTATCAA	1980	0.0	22.61111	1
TATACTG	135	0.0	21.925924	5
GCACCGC	255	0.0	21.764708	10
TTAACGG	255	0.0	21.764708	35
AACGGCC	265	0.0	20.943396	37
CGTGGAT	45	0.003824836	20.555557	12
TAACGGC	275	0.0	19.50909	36
TATTAGA	315	0.0	19.380953	2
ATTAGAG	325	0.0	19.353846	3
CACCGCC	280	0.0	19.160713	11
GGTCGCC	185	0.0	19.0	36
AGGTCGC	185	0.0	19.0	35
GTCGCCC	195	0.0	18.025642	37
GTATTAG	355	0.0	17.718311	1
TCTTACC	225	0.0	17.266666	2
GTGACAC	300	0.0	17.266666	24
TCTAGAC	65	0.0015793837	17.076923	3
TTAGAGG	425	0.0	16.97647	4
ACGAGGG	285	0.0	16.877193	15
>>END_MODULE