FastQCFastQC Report
Fri 10 Feb 2017
ERR1633261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732582
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37330.5095675296417329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27110.37006096245881004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15380.20994236822635556No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8960.12230712739324744No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8270.11288838655604423No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7590.10360614920923528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGT250.005495057429.620
GGTATCA7700.027.6298731
GTATCAA18800.020.6648941
TGCGGTA656.897764E-519.92307736
TTGACGC609.232457E-418.532
TGTACGG1054.7914E-717.6190475
TCGCGCG852.720933E-517.41176427
AGTGACA2450.017.36734623
GTGACAC2150.017.20930324
CCTACCG1301.3906174E-817.07692324
AATTCCG650.001579194717.0769235
TTAACGG2100.016.73809435
CGCGCGC1005.871936E-616.65000228
CGGGTCA1452.9722287E-916.58620617
GTATTAG3150.016.4444451
TATCGCG904.4424138E-516.44444525
CGAGTCG1251.6551894E-716.2821
AACGGCC2050.016.24390237
TAATATA1951.8189894E-1216.1282044
TTTCGGG1059.333771E-615.85714222
Produced by FastQC (version 0.11.5)