FastQCFastQC Report
Fri 10 Feb 2017
ERR1633260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870576
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT219372.519825954310709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161001.849350315193619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87691.0072641561448972No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA48390.5558388928709268No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA39740.45647938835897156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.45245906158681143No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG34500.3962893532557755No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG32820.3769917847494073No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30210.3470116336770138No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT29840.34276157394644463No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC26880.3087610961018912No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT23830.2737268199444965No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22800.26189557258642554No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT22610.2597131094815387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.2529359872084689No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC21710.2493751263531271No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC20810.23903714322471561No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG20320.23340868574369153No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20020.22996269136755434No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA19430.22318556909448456No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG19410.22295583613607542No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18770.2156043814669828No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC18710.21491518259175532No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC18670.21445571667493704No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC18360.21089485581959527No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT18120.2081380603186856No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA17940.20607046369300325No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16830.19332028450129568No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16720.1920567532300454No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA16300.18723236110345334No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA16030.18413096616492988No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA15880.1824079689768613No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA15740.18079983826799728No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC15550.1786173751631104No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14970.1719551193692452No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14920.17138078697322232No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14490.1664415283674257No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT14440.16586719597140284No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14310.1643739317417434No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14270.1639144658249251No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13850.15909007369833306No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT13850.15909007369833306No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG13510.1551846134053776No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13460.15461028100935473No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG13330.15311701677969528No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13020.14955615592435353No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12970.14898182352833067No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC12870.14783315873628494No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12780.1467993604234438No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12760.14656962746503463No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.1458804285898072No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA12650.14530609619378435No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT12470.14323849956810203No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG12390.14231956773446544No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG12370.1420898347760563No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA12330.14163036885923802No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12240.14059657054639685No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12230.1404817040671923No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG12170.13979250519196487No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12060.13852897392071456No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11960.13738030912866883No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG11740.13485324658616824No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.1337045817941225No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG11550.13267078348128136No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11420.1311775192516219No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11370.13060318685559907No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11270.12945452206355332No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT11260.12933965558434876No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10840.1245152634577567No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10760.12359633162412012No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10470.12026520372718753No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10310.11842734005991434No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC10270.11796787414309606No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.11716380878866405No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9960.11440701328775432No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC9960.11440701328775432No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9860.1132583484957086No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9860.1132583484957086No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC9660.11096101891161714No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9650.11084615243241255No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9550.10969748764036683No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9530.10946775468195769No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA9450.10854882284832112No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT9380.1077447574938891No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9290.10671095918104795No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9250.10625149326422965No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9220.10590689382661594No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9220.10590689382661594No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA9110.10464336255536565No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9030.10372443072172906No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC8990.10326496480491078No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8950.10280549888809248No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG8930.10257576592968334No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT8900.1022311664920696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCA200.00184169137.05
ATGCCAT200.00184169137.06
CTAAGTC352.3876475E-531.71428734
TATCTAT551.8546416E-830.2727287
CGCGGAG250.005495620429.615
TTATGCC250.005495620429.64
ATGTACC250.005495620429.629
TATTGCG502.7194983E-729.611
TTGCGGT502.7194983E-729.635
TAGATTT250.005495620429.68
TGCACTA250.005495620429.613
GTACTAT454.006788E-628.7777791
CCTCCAC405.9385005E-527.751
ATACGGG405.9385005E-527.753
TAGTACA405.9385005E-527.7510
TCCCTTG604.3202817E-827.74999831
GAGCTAT556.258815E-726.9090913
TTTCAAT556.258815E-726.9090923
CTAGTGC706.5883796E-926.4285729
ACTCCAA358.8676595E-426.42857220
Produced by FastQC (version 0.11.5)