FastQCFastQC Report
Fri 10 Feb 2017
ERR1633259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896012
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46380.517626996067017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32640.3642808355245242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19180.2140596331299134No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18590.20747489988973364No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC16210.18091275563273707No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC14580.16272103498613857No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13070.14586858211720377No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA12320.13749815850680572No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12010.13403838341450786No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA11790.13158305915545776No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC11670.13024379137779404No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10870.12131533952670277No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG10830.1208689169341482No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10420.11629308536046391No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10250.11439578934210703No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT9520.10624857702798624No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC9390.1047977036021839No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA9260.10334683017638156No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT9160.10223077369499517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGC250.005495704229.69
GGTATCA10100.024.7277241
GCCGGCA1900.024.34210615
CGCTCTC1750.024.31428529
TTCGCCG1700.023.94117524
GTATCAA26900.022.4200741
GCCGCTC1900.022.39473727
CCGCTCT2100.022.0238128
CCGGCAG2050.021.65853516
TTGCGGT603.7251055E-521.58333435
AGCTTCG2000.021.27500221
CTGCTCG3550.020.845079
ACCGAGC909.472387E-820.55555527
CTACCGA909.472387E-820.55555525
GTCGCCC1351.8189894E-1220.55555337
GCGACAA1001.288754E-820.3516
TAGACCC555.141878E-420.1818184
TGCTCGC2300.020.10869610
GCTTCGC2050.019.85365722
TCGCCAT2300.018.513
Produced by FastQC (version 0.11.5)