FastQCFastQC Report
Fri 10 Feb 2017
ERR1633258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074834
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50640.47114252061248524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.31753740577614775No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19470.1811442511122648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19460.18105121348971098No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14210.1322064616489616No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12430.11564576483438373No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12120.11276159853521567No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11740.10922616887817096No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10860.10103885809343582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.024.3240741
CGAATTA551.9025096E-523.54545415
GTATCAA27950.022.7030411
ACGTATA603.725997E-521.58333229
TTGCGGT957.1431714E-921.42105335
GCCGGCA2000.021.27500215
TTCGCCG2050.020.756124
CGCTCTA450.003825649620.55555514
GACGTAT656.901597E-519.92307728
CCGAATT656.901597E-519.92307714
GCCGCTC2150.019.79069727
GTATAAC851.2451546E-619.5882361
GCTTCGC2300.018.522
ACAATCG609.2362845E-418.537
GGACGTA701.2191041E-418.527
AAACACG801.6165111E-518.515
ACGGTGT609.2362845E-418.535
GCTTTAT4700.018.1063841
AACGGCC3200.017.92187537
TTAACGG3300.017.37878835
Produced by FastQC (version 0.11.5)