##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1074834
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89671242256944	33.0	33.0	33.0	33.0	33.0
2	32.013121095908765	33.0	33.0	33.0	33.0	33.0
3	31.724657016804457	33.0	33.0	33.0	27.0	33.0
4	32.010128075591204	33.0	33.0	33.0	33.0	33.0
5	32.11393201182694	33.0	33.0	33.0	33.0	33.0
6	35.295773114732135	37.0	37.0	37.0	33.0	37.0
7	35.435439332957465	37.0	37.0	37.0	33.0	37.0
8	35.56974565374746	37.0	37.0	37.0	33.0	37.0
9	35.682791947407694	37.0	37.0	37.0	33.0	37.0
10	35.69605259975029	37.0	37.0	37.0	33.0	37.0
11	35.71163174964692	37.0	37.0	37.0	33.0	37.0
12	35.69683504615597	37.0	37.0	37.0	33.0	37.0
13	35.703383034031305	37.0	37.0	37.0	33.0	37.0
14	35.68362091262465	37.0	37.0	37.0	33.0	37.0
15	35.69734117082266	37.0	37.0	37.0	33.0	37.0
16	35.678883436884206	37.0	37.0	37.0	33.0	37.0
17	35.68175085641131	37.0	37.0	37.0	33.0	37.0
18	35.66357409609298	37.0	37.0	37.0	33.0	37.0
19	35.65593012502396	37.0	37.0	37.0	33.0	37.0
20	35.64495912857241	37.0	37.0	37.0	33.0	37.0
21	35.648964398223356	37.0	37.0	37.0	33.0	37.0
22	35.563096254863545	37.0	37.0	37.0	33.0	37.0
23	35.60460685091837	37.0	37.0	37.0	33.0	37.0
24	35.599437680609284	37.0	37.0	37.0	33.0	37.0
25	35.62075818219372	37.0	37.0	37.0	33.0	37.0
26	35.51862520165905	37.0	37.0	37.0	33.0	37.0
27	35.525941680296675	37.0	37.0	37.0	33.0	37.0
28	35.55027380972317	37.0	37.0	37.0	33.0	37.0
29	35.56060935921268	37.0	37.0	37.0	33.0	37.0
30	35.553318000733135	37.0	37.0	37.0	33.0	37.0
31	35.5437974608172	37.0	37.0	37.0	33.0	37.0
32	35.546006173976636	37.0	37.0	37.0	33.0	37.0
33	35.53526684120525	37.0	37.0	37.0	33.0	37.0
34	35.516820271781505	37.0	37.0	37.0	33.0	37.0
35	35.45520889737392	37.0	37.0	37.0	33.0	37.0
36	35.481906043165736	37.0	37.0	37.0	33.0	37.0
37	35.4792088824879	37.0	37.0	37.0	33.0	37.0
38	35.46948738130725	37.0	37.0	37.0	33.0	37.0
39	35.366228645539685	37.0	37.0	37.0	33.0	37.0
40	35.168756291669226	37.0	37.0	37.0	33.0	37.0
41	35.338491339127714	37.0	37.0	37.0	33.0	37.0
42	35.373291131467745	37.0	37.0	37.0	33.0	37.0
43	35.04714588485292	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	28.0
16	50.0
17	45.0
18	33.0
19	33.0
20	60.0
21	172.0
22	442.0
23	981.0
24	2093.0
25	3892.0
26	6375.0
27	9750.0
28	13765.0
29	18752.0
30	24932.0
31	32417.0
32	41834.0
33	55695.0
34	80243.0
35	166342.0
36	616894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75314513683043	20.36695899087673	13.677088741145143	24.202807131147694
2	16.147702808061524	21.519136908583093	38.63415187833657	23.69900840501882
3	18.05367154369884	27.323661142092636	29.949927151541537	24.67274016266698
4	12.37753922931355	17.8193097724858	40.089911558436	29.71323943976465
5	13.827623614437206	36.90439639981616	35.44165889802519	13.826321087721452
6	29.131754298803347	40.20965097866275	17.171581844266186	13.487012878267715
7	26.53432995234613	32.144033404228	23.44296886775074	17.878667775675126
8	24.871003336329146	34.660235906195744	20.831588877910452	19.637171879564658
9	26.40928738763381	15.319667967332629	20.128317489026212	38.142727156007346
10	15.415496718563052	27.89751719800453	33.29416449423818	23.392821589194238
11	33.86039146510066	24.022035030525643	23.94602329289918	18.171550211474514
12	23.708033054406542	25.884741271675438	29.462037858869373	20.945187815048648
13	28.291810642387567	22.308002910216835	25.779050532454313	23.621135914941284
14	22.19626472552971	21.07125379360906	27.946548025090383	28.785933455770845
15	24.309614321839465	28.5991139096828	24.56918928876459	22.522082479713145
16	22.718577938546787	28.25645634581712	25.30251182973371	23.722453885902382
17	22.72853296416005	27.223924810714955	26.061047566414906	23.98649465871009
18	23.21111911234665	26.78301951743246	27.928870876805163	22.076990493415728
19	25.539199541510598	25.842595228658567	27.543788157054948	21.07441707277589
20	25.050565947857994	25.73122919446166	27.313520041234273	21.904684816446075
21	23.29736498845403	26.678166116814317	27.649199783408413	22.375269111323238
22	22.86446093071116	26.81772255064503	27.639337795417713	22.678478723226096
23	23.018996421773036	26.47050614327422	27.88021220020952	22.630285234743226
24	22.882417191864047	26.98881873852148	26.881174209226728	23.247589860387745
25	23.277454937227514	27.0784139690408	27.371761592952957	22.272369500778723
26	23.410033549366695	26.889268482388907	27.410930432048115	22.289767536196287
27	23.205536854993422	26.698634393776153	27.13730678411736	22.95852196711306
28	22.801474460242233	26.671188295122782	27.731445041745985	22.795892202889004
29	24.018499600868598	26.81949026547355	26.919877860209112	22.242132273448735
30	23.723291224505367	26.254379746081717	27.368412238541023	22.653916790871893
31	23.051931740157084	26.778925862040094	27.455774566119047	22.713367831683776
32	22.126486508614356	26.585500644750727	27.77173033231178	23.51628251432314
33	21.986278811425763	26.667652865465737	28.251339276576665	23.094729046531835
34	23.348535680858625	26.13584981494817	27.663899727771916	22.85171477642129
35	22.88623173438875	26.857914803588272	27.133957429705426	23.121896032317547
36	23.43831698662305	26.73510514181725	27.308309934371263	22.51826793718844
37	23.354583126324624	26.15985352156705	27.409720942954912	23.075842409153413
38	23.4760902613799	26.542703338375972	27.126235307033458	22.854971093210672
39	22.94800871576448	26.49013708163307	27.428607580333335	23.133246622269112
40	22.79403145043793	26.97895675053078	27.665016179242564	22.56199561978873
41	22.68238630337336	26.10003033026495	27.593656322743794	23.623927043617897
42	22.326796509972702	27.079809533379105	27.53206541661317	23.06132854003502
43	23.29634157460594	26.321459871943016	27.25285951132919	23.129339042121853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	56.0
2	73.0
3	304.0
4	535.0
5	535.0
6	658.0
7	781.0
8	709.0
9	637.0
10	925.0
11	1213.0
12	1213.0
13	1909.5
14	2606.0
15	4527.5
16	6449.0
17	7452.5
18	8456.0
19	8456.0
20	7968.5
21	7481.0
22	8202.5
23	8924.0
24	11732.0
25	14540.0
26	14540.0
27	18518.5
28	22497.0
29	26708.5
30	30920.0
31	36127.5
32	41335.0
33	41335.0
34	48983.0
35	56631.0
36	60932.5
37	65234.0
38	69159.0
39	73084.0
40	73084.0
41	76261.0
42	79438.0
43	81022.0
44	82606.0
45	82049.5
46	81493.0
47	81493.0
48	80775.0
49	80057.0
50	79507.5
51	78958.0
52	75935.0
53	72912.0
54	72912.0
55	69513.0
56	66114.0
57	57515.5
58	48917.0
59	46248.0
60	43579.0
61	43579.0
62	38678.0
63	33777.0
64	28642.0
65	23507.0
66	19733.0
67	15959.0
68	15959.0
69	13448.0
70	10937.0
71	9229.0
72	7521.0
73	5452.5
74	3384.0
75	3384.0
76	2543.0
77	1702.0
78	1309.5
79	917.0
80	751.5
81	586.0
82	586.0
83	498.5
84	411.0
85	360.5
86	310.0
87	241.5
88	173.0
89	173.0
90	140.0
91	107.0
92	70.0
93	33.0
94	17.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1074834.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.72529683476963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.13797217781647	45.41650151820501
2	8.068718748220165	8.508511821523582
3	1.9953966051944754	3.1562363493591103
4	0.8904924752492608	1.878059203465841
5	0.5222999427773163	1.3769209759858596
6	0.3391171088896997	1.0728030136774989
7	0.2543288309675897	0.9386694174484282
8	0.18980305057493221	0.8005937745366475
9	0.15215488384415193	0.7220170273988524
>10	1.1462557136878118	12.220322264076719
>50	0.16533515305832067	6.043265228758639
>100	0.12756910093764934	12.822359452022782
>500	0.008588102060186882	3.104297135584787
>1k	0.0017891879292056005	1.4617280249152542
>5k	1.7891879292056002E-4	0.4777147930410357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5064	0.47114252061248524	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3413	0.31753740577614775	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1947	0.1811442511122648	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1946	0.18105121348971098	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1421	0.1322064616489616	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1243	0.11564576483438373	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1212	0.11276159853521567	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1174	0.10922616887817096	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1086	0.10103885809343582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.30376225538083E-5	0.0	0.0
2	0.0	0.0	9.30376225538083E-5	0.0	0.0
3	0.0	0.0	1.860752451076166E-4	0.0	0.0
4	0.0	0.0	3.721504902152332E-4	0.0	0.0
5	0.0	0.0	5.582257353228499E-4	9.30376225538083E-5	0.0
6	9.30376225538083E-5	0.0	0.001209489093199508	2.7911286766142495E-4	0.0
7	9.30376225538083E-5	0.0	0.0037215049021523325	4.6518811276904156E-4	0.0
8	1.860752451076166E-4	0.0	0.004186693014921374	5.582257353228499E-4	0.0
9	1.860752451076166E-4	0.0	0.004930993995351841	0.0013025267157533162	0.0
10	5.582257353228499E-4	0.0	0.005768332598336115	0.0013955643383071247	0.0
11	6.512633578766581E-4	0.0	0.009396799877934639	0.0015816395834147413	0.0
12	6.512633578766581E-4	0.0	0.010141100858365107	0.0015816395834147413	0.0
13	6.512633578766581E-4	0.0	0.010885401838795572	0.0015816395834147413	0.0
14	6.512633578766581E-4	0.0	0.011350589951564613	0.0015816395834147413	0.0
15	6.512633578766581E-4	0.0	0.012746154289871739	0.0015816395834147413	0.0
16	6.512633578766581E-4	0.0	0.013397417647748397	0.0016746772059685496	0.0
17	6.512633578766581E-4	0.0	0.013583492892856014	0.0017677148285223578	0.0
18	6.512633578766581E-4	0.0	0.013862605760517437	0.0018607524510761662	0.0
19	6.512633578766581E-4	0.0	0.014048681005625055	0.0019537900736299745	0.0
20	6.512633578766581E-4	0.0	0.014234756250732672	0.0020468276961837827	0.0
21	6.512633578766581E-4	0.0	0.014513869118394097	0.0025120158089528243	0.0
22	6.512633578766581E-4	0.0	0.014606906740947905	0.002977203921721866	0.0
23	6.512633578766581E-4	0.0	0.014979057231163138	0.0035354296570447156	0.0
24	6.512633578766581E-4	0.0	0.015072094853716946	0.004279730637475182	0.0
25	6.512633578766581E-4	0.0	0.015072094853716946	0.0043727682600289905	0.0
26	6.512633578766581E-4	0.0	0.015072094853716946	0.0050240316179056485	0.0
27	6.512633578766581E-4	0.0	0.015072094853716946	0.005954407843443732	0.0
28	6.512633578766581E-4	0.0	0.015072094853716946	0.013118304780086971	0.0
29	6.512633578766581E-4	0.0	0.015072094853716946	0.03265620551638672	0.0
30	6.512633578766581E-4	0.0	0.015072094853716946	0.05917192794422208	0.0
31	6.512633578766581E-4	0.0	0.015072094853716946	0.13127608542342353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1080	0.0	24.324074	1
CGAATTA	55	1.9025096E-5	23.545454	15
GTATCAA	2795	0.0	22.703041	1
ACGTATA	60	3.725997E-5	21.583332	29
TTGCGGT	95	7.1431714E-9	21.421053	35
GCCGGCA	200	0.0	21.275002	15
TTCGCCG	205	0.0	20.7561	24
CGCTCTA	45	0.0038256496	20.555555	14
GACGTAT	65	6.901597E-5	19.923077	28
CCGAATT	65	6.901597E-5	19.923077	14
GCCGCTC	215	0.0	19.790697	27
GTATAAC	85	1.2451546E-6	19.588236	1
GCTTCGC	230	0.0	18.5	22
ACAATCG	60	9.2362845E-4	18.5	37
GGACGTA	70	1.2191041E-4	18.5	27
AAACACG	80	1.6165111E-5	18.5	15
ACGGTGT	60	9.2362845E-4	18.5	35
GCTTTAT	470	0.0	18.106384	1
AACGGCC	320	0.0	17.921875	37
TTAACGG	330	0.0	17.378788	35
>>END_MODULE