FastQCFastQC Report
Fri 10 Feb 2017
ERR1633257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143141
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7280.5085894327970323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.4429199181226902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5030.3514017646935539No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2050.14321543093872474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1299418056322088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC200.001836556537.011
GGTATCA2300.029.760871
TCTTCCC250.00548047629.629
GGACACT250.00548047629.66
GTACATG405.9044112E-527.7500021
GCGAACT451.31532E-424.66666628
AAAGCGA400.001922980623.12500225
CGAACTA502.6861168E-422.229
AAGCGAA450.00380970620.55555526
CAAAGCG450.00380970620.55555524
CATGCTA555.1129155E-420.1818184
ACCAGTC555.1129155E-420.18181818
GTATCAA5250.019.7333341
ACTATAC609.1830594E-418.535
AGTCAAA500.00700555918.521
GAACTAC609.1830594E-418.530
ACTTCAC701.2097595E-418.513
TACATGC609.1830594E-418.52
TAAGACT609.1830594E-418.59
CACCAGT609.1830594E-418.517
Produced by FastQC (version 0.11.5)