##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633257.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143141
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.148923089820528	33.0	27.0	33.0	14.0	33.0
2	30.18170894432762	33.0	33.0	33.0	14.0	33.0
3	30.115620262538336	33.0	33.0	33.0	14.0	33.0
4	30.412690983016745	33.0	33.0	33.0	14.0	33.0
5	30.57912827212329	33.0	33.0	33.0	27.0	33.0
6	30.473393367379018	37.0	27.0	37.0	14.0	37.0
7	30.87420096268714	37.0	27.0	37.0	14.0	37.0
8	30.962463584856888	37.0	27.0	37.0	14.0	37.0
9	30.56586163293536	37.0	27.0	37.0	14.0	37.0
10	30.536547879363706	37.0	27.0	37.0	14.0	37.0
11	30.65184678044725	37.0	27.0	37.0	14.0	37.0
12	30.92334830691416	37.0	27.0	37.0	14.0	37.0
13	31.156978084546008	37.0	27.0	37.0	14.0	37.0
14	30.992308283440803	37.0	27.0	37.0	14.0	37.0
15	31.204113426621305	37.0	27.0	37.0	14.0	37.0
16	31.296113622232625	37.0	27.0	37.0	14.0	37.0
17	31.247329556171888	37.0	27.0	37.0	14.0	37.0
18	31.256257815720165	37.0	27.0	37.0	14.0	37.0
19	31.14749093551114	37.0	27.0	37.0	14.0	37.0
20	31.204406843601763	37.0	27.0	37.0	14.0	37.0
21	31.248915405090084	37.0	27.0	37.0	14.0	37.0
22	31.08711689872224	37.0	27.0	37.0	14.0	37.0
23	31.08914985922971	37.0	27.0	37.0	14.0	37.0
24	31.103331679951935	37.0	27.0	37.0	14.0	37.0
25	31.08332343633201	37.0	27.0	37.0	14.0	37.0
26	30.922922153680638	37.0	27.0	37.0	14.0	37.0
27	30.922978042629296	37.0	27.0	37.0	14.0	37.0
28	30.87540257508331	37.0	27.0	37.0	14.0	37.0
29	30.92937732725075	37.0	27.0	37.0	14.0	37.0
30	30.92227943077106	37.0	27.0	37.0	14.0	37.0
31	30.963225071782368	37.0	27.0	37.0	14.0	37.0
32	30.881159136795187	37.0	27.0	37.0	14.0	37.0
33	30.83296889081395	37.0	27.0	37.0	14.0	37.0
34	30.76793511293061	37.0	27.0	37.0	14.0	37.0
35	30.773747563591144	37.0	27.0	37.0	14.0	37.0
36	30.798198978629465	37.0	27.0	37.0	14.0	37.0
37	30.813582411747856	37.0	27.0	37.0	14.0	37.0
38	30.821364947848625	37.0	27.0	37.0	14.0	37.0
39	30.67900182337695	37.0	27.0	37.0	14.0	37.0
40	30.47266681104645	37.0	27.0	37.0	14.0	37.0
41	30.699436220230403	37.0	27.0	37.0	14.0	37.0
42	30.664044543492082	37.0	27.0	37.0	14.0	37.0
43	30.1074674621527	33.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	21.0
17	41.0
18	130.0
19	291.0
20	701.0
21	1345.0
22	2129.0
23	3472.0
24	4770.0
25	5922.0
26	7505.0
27	8909.0
28	10209.0
29	11195.0
30	11942.0
31	12510.0
32	12946.0
33	12848.0
34	12341.0
35	11944.0
36	11957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.853962177153996	23.515973760138607	10.495245946304694	22.13481811640271
2	17.034951551267632	27.64057817117388	33.00382140686456	22.320648870693933
3	20.277209185348713	29.74130402889459	28.735302953032328	21.246183832724377
4	15.347105301765392	23.927456144640598	35.63968394799533	25.085754605598677
5	17.38495609224471	37.31216073661635	32.96679497837796	12.336088192760984
6	27.842476998204567	41.11959536401171	17.278068477934344	13.75985915984938
7	25.686560803683083	34.26621303470005	22.888620311441166	17.1586058501757
8	24.685450010828482	36.47801817788055	20.314934225693545	18.52159758559742
9	25.99395002130766	16.18613814350885	20.718731879754927	37.10117995542856
10	15.35688586778072	29.650484487323688	32.88715322653887	22.105476418356726
11	32.79563507310973	24.70780559029209	24.44303169602001	18.05352764057817
12	22.87464807427641	27.34227090770639	30.350493569277848	19.432587448739355
13	28.629113950580198	23.048602426977595	26.40682962952613	21.915453992916074
14	21.54658693176658	22.597997778414292	28.908558693875268	26.946856595943856
15	23.682941994257412	29.639306697591884	24.950223905100565	21.72752740305014
16	21.640899532628666	29.359163342438578	26.536072823300106	22.463864301632654
17	21.62203701245625	28.68500289923921	27.382790395484175	22.310169692820367
18	22.36326419404643	27.50504747067577	28.779315500101298	21.352372835176503
19	24.23205091483223	27.24865691870254	28.7478779664806	19.77141419998463
20	24.22576340810809	26.870009291537716	28.53759579715106	20.366631503203138
21	22.908181443471822	27.684590718242852	28.705961254986338	20.701266583298985
22	22.16415981444869	27.98080214613563	28.69548207711278	21.159555962302903
23	22.200487631077053	27.460336311748556	29.027322709775678	21.311853347398717
24	21.77573162126854	27.968925744545587	28.487994355216184	21.767348278969685
25	22.361866970329956	28.119127294066686	28.55575970546524	20.963246030138116
26	22.461069854199707	27.9284062567678	28.42092761682537	21.189596272207126
27	22.228432105406558	27.776807483530224	28.640291740312	21.354468670751217
28	21.964356822992713	27.794971391844403	28.636798681020814	21.603873104142068
29	22.350689180598152	27.783094990254366	28.32940946339623	21.53680636575125
30	22.391907280234175	27.430296001844333	28.851971133358017	21.325825584563475
31	22.60777834442962	27.660139303204534	28.446077643721924	21.286004708643922
32	21.605270327858545	27.83479226776395	28.734604341174087	21.825333063203413
33	21.60736616343326	28.004554949315708	29.109060297189483	21.279018590061547
34	21.93222067751378	27.639879559315638	28.890394785561092	21.53750497760949
35	22.11875004366324	27.710439356997647	28.831711389469124	21.33909920986999
36	22.28152660663262	28.05764945054177	28.32451918038857	21.336304762437038
37	22.79989660544498	27.49806135209339	28.419530393108893	21.282511649352738
38	22.42963232057901	27.956350731097306	28.096073102744846	21.517943845578834
39	22.115256984372053	27.862038130235224	28.468433223185528	21.554271662207196
40	22.115256984372053	28.153359275120337	28.81075303372199	20.920630706785616
41	22.396797563241837	27.486883562361587	28.163139841135663	21.953179033260913
42	21.75407465366317	28.08908698416247	28.395777589928812	21.761060772245546
43	22.385619773510037	27.68249488266814	28.11214117548431	21.81974416833751
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	8.0
3	26.5
4	45.0
5	45.0
6	76.0
7	107.0
8	122.5
9	138.0
10	187.5
11	237.0
12	237.0
13	396.5
14	556.0
15	992.0
16	1428.0
17	1555.5
18	1683.0
19	1683.0
20	1724.5
21	1766.0
22	1860.0
23	1954.0
24	2435.5
25	2917.0
26	2917.0
27	3530.5
28	4144.0
29	4755.5
30	5367.0
31	6061.0
32	6755.0
33	6755.0
34	7590.0
35	8425.0
36	8801.5
37	9178.0
38	9336.0
39	9494.0
40	9494.0
41	9870.5
42	10247.0
43	10249.5
44	10252.0
45	10165.5
46	10079.0
47	10079.0
48	9976.0
49	9873.0
50	9780.5
51	9688.0
52	9616.5
53	9545.0
54	9545.0
55	8821.0
56	8097.0
57	7255.0
58	6413.0
59	5680.5
60	4948.0
61	4948.0
62	4326.0
63	3704.0
64	2994.5
65	2285.0
66	1891.0
67	1497.0
68	1497.0
69	1217.0
70	937.0
71	790.0
72	643.0
73	480.5
74	318.0
75	318.0
76	228.0
77	138.0
78	116.0
79	94.0
80	78.0
81	62.0
82	62.0
83	50.5
84	39.0
85	40.0
86	41.0
87	29.0
88	17.0
89	17.0
90	13.0
91	9.0
92	7.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	143141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.80421422048113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.09298620138313	70.73129440414843
2	4.121951199439646	6.331664458562634
3	1.2655450209977823	2.915975726951309
4	0.6327336558892536	1.943864450057057
5	0.4337296833189391	1.6656133755704616
6	0.2958082680720724	1.3631592953518175
7	0.23084966290680872	1.2411158873834276
8	0.14090818252456158	0.8657873788828065
9	0.10493162039676132	0.7253271586309556
>10	0.6425812522235604	8.689342550070231
>50	0.029980462774315476	1.5358514655269062
>100	0.004996743795719245	0.5595380901394876
>500	0.0029980462774315472	1.431465758724478
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	728	0.5085894327970323	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	634	0.4429199181226902	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	503	0.3514017646935539	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	205	0.14321543093872474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.1299418056322088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	6.986118582376817E-4	0.0	0.0
6	0.0	0.0	6.986118582376817E-4	0.0	0.0
7	0.0	0.0	6.986118582376817E-4	0.0	0.0
8	0.0	0.0	6.986118582376817E-4	0.0	0.0
9	0.0	0.0	0.0013972237164753634	0.0	0.0
10	0.0	0.0	0.0013972237164753634	0.0	0.0
11	0.0	0.0	0.0020958355747130453	0.0	0.0
12	0.0	0.0	0.0020958355747130453	0.0	0.0
13	0.0	0.0	0.0020958355747130453	0.0	0.0
14	0.0	0.0	0.0020958355747130453	0.0	0.0
15	6.986118582376817E-4	0.0	0.0027944474329507267	0.0	0.0
16	6.986118582376817E-4	0.0	0.0027944474329507267	0.0	0.0
17	6.986118582376817E-4	0.0	0.0027944474329507267	0.0	0.0
18	6.986118582376817E-4	0.0	0.0034930592911884086	0.0	0.0
19	6.986118582376817E-4	0.0	0.0034930592911884086	0.0	0.0
20	6.986118582376817E-4	0.0	0.0034930592911884086	0.0	0.0
21	6.986118582376817E-4	0.0	0.0034930592911884086	0.0	0.0
22	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
23	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
24	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
25	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
26	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
27	0.0013972237164753634	0.0	0.0034930592911884086	0.0013972237164753634	0.0
28	0.0013972237164753634	0.0	0.0034930592911884086	0.004890283007663772	0.0
29	0.0013972237164753634	0.0	0.0034930592911884086	0.01187640159004059	0.0
30	0.0013972237164753634	0.0	0.0034930592911884086	0.018163908314179723	0.0
31	0.0013972237164753634	0.0	0.0034930592911884086	0.041916711494260905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCC	20	0.0018365565	37.0	11
GGTATCA	230	0.0	29.76087	1
TCTTCCC	25	0.005480476	29.6	29
GGACACT	25	0.005480476	29.6	6
GTACATG	40	5.9044112E-5	27.750002	1
GCGAACT	45	1.31532E-4	24.666666	28
AAAGCGA	40	0.0019229806	23.125002	25
CGAACTA	50	2.6861168E-4	22.2	29
AAGCGAA	45	0.003809706	20.555555	26
CAAAGCG	45	0.003809706	20.555555	24
CATGCTA	55	5.1129155E-4	20.181818	4
ACCAGTC	55	5.1129155E-4	20.181818	18
GTATCAA	525	0.0	19.733334	1
ACTATAC	60	9.1830594E-4	18.5	35
AGTCAAA	50	0.007005559	18.5	21
GAACTAC	60	9.1830594E-4	18.5	30
ACTTCAC	70	1.2097595E-4	18.5	13
TACATGC	60	9.1830594E-4	18.5	2
TAAGACT	60	9.1830594E-4	18.5	9
CACCAGT	60	9.1830594E-4	18.5	17
>>END_MODULE