FastQCFastQC Report
Fri 10 Feb 2017
ERR1633256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036483
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38070.3672998013474413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.2572159890707325No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG16960.16363027661814036No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA15610.1506054609675219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14850.14327297215680335No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14100.1360369634620153No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG13760.13275663952037806No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG12960.12503823024593747No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12320.118863502826385No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11250.10854013042182072No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11230.1083471701899597No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT10570.1019794825385462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.025.7005981
AACGGCC2500.023.6837
TTAACGG2600.022.7692335
GTATCAA23050.022.0715851
TAACGGC2950.020.06779736
GGACCGT759.264331E-619.7333346
TGCGGTA1355.638867E-1119.18518636
TTTAACG3200.019.07812534
CCGTAGT609.2359824E-418.527
GTTTAAC3400.017.95588333
CATAATA2900.017.8620682
TCGTACT1156.4030246E-817.69565226
CGAGTCG953.6056954E-617.52631621
AGCTTCG1603.45608E-1117.3437521
TGCTCGC1603.45608E-1117.3437510
CTTGCGG1502.5102054E-1017.26666634
CACATGT3650.017.23287628
GTATTAG5950.017.100841
TTGCGGT1554.0017767E-1016.70967935
ACATGTT3800.016.55263129
Produced by FastQC (version 0.11.5)