FastQCFastQC Report
Fri 10 Feb 2017
ERR1633255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719429
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160352.228850935950594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115931.6114168319597901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63130.8775014629657687No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA53090.7379463435585721No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA44200.614376123286662No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG41950.5831013206306668No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA36050.5010918381105015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.4194993529590828No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT29830.41463438365703914No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT26860.3733516441511254No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC24720.34360583184720106No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT24560.34138184588055254No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC23670.32901092394106995No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC23470.3262309414827593No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC21330.2964851291788349No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA21190.2945391414580174No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG20800.2891181756643115No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20640.286894189697663No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA20420.28383620899352124No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20420.28383620899352124No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19680.2735502738977717No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18430.2561753835333299No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA18430.2561753835333299No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT17900.2488084300188066No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC17830.24783543615839784No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG17220.23935648966055023No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17050.23699350457098617No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16620.2310165422856182No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT16590.23059954491687157No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.2299045493022939No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG16400.22795856158147643No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT16330.22698556772106768No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC16020.22267659491068612No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG15670.21781162560864242No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG15190.21113966770869674No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14900.2071086931441463No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14630.20335571682542683No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14610.20307771857959578No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG14520.20182672647335595No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT14470.20113173085877828No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14100.19598876331090354No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14030.19501576945049476No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA13760.19126279313177535No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13750.1911237940088598No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG13740.19098479488594428No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA13230.183895839617252No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG12770.17750187996313743No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA12730.1769458834714753No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG12630.17555589224231996No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT12410.1724979115381782No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12320.17124691943193837No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG12100.1681889387277966No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG12050.16749394311321894No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12030.1672159448673879No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT11730.16304597117992187No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11540.1604049878445267No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC11380.15818100187787815No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT11290.15693000977163835No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11200.15567901766539852No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA10850.15081404836335482No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG10840.15067504924043928No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10750.14942405713419948No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT10680.14845106327379076No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC10560.14678307379880434No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10450.14525408344673343No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10270.14275209923425383No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10140.14094511063635187No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT10080.1401111158988587No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG9820.13649713870305477No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9710.13496814835098392No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT9410.13079817466351787No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9310.12940818343436253No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9300.129269184311447No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9200.12787919308229165No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9150.12718419746771398No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA9120.12676720009896736No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9080.12621120360730526No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1256552071156431No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC9040.1256552071156431No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9000.12509921062398097No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC8920.12398721764065668No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8850.12301422378024796No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG8850.12301422378024796No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8810.12245822728858581No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC8570.11912224833861297No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8550.11884425009278192No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.1184272527240353No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG8490.11801025535528871No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG8480.11787125623237318No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG8180.11370128254490715No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8150.11328428517616054No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.11245029043866733No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8020.11147729657825858No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA7970.11078230096368091No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA7970.11078230096368091No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7800.10841931587411685No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7720.10730732289079255No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT7720.10730732289079255No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7650.10633432903038383No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA7640.10619532990746829No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG7530.1046663395553974No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7490.10411034306373526No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA7370.10244235358874884No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC7260.10091336323667797No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7230.10049636586793138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC603.45608E-1133.9166643
TAACAAC555.002221E-1033.6363641
TAAGGAT401.5989663E-632.37524
CCACTAG352.3870758E-531.7142853
TGTCAAC303.5981525E-430.8333322
TGTGGTT303.5981525E-430.83333212
CTAAGTC250.005494992729.634
GACATCT454.0055183E-628.77777718
AACAACG652.9867806E-928.4615382
CTAGTGC652.9867806E-928.4615389
TTATAGA405.9370883E-527.754
TAGATCT405.9370883E-527.757
GGACCCA405.9370883E-527.7534
TAGTGCA604.318099E-827.74999810
TATTTCC556.256287E-726.9090917
CGCGGGA556.256287E-726.909098
CTATTTC556.256287E-726.9090916
ATTTCCT556.256287E-726.9090918
ATGTGGT358.8661583E-426.4285711
GCCTACA358.8661583E-426.4285733
Produced by FastQC (version 0.11.5)