FastQCFastQC Report
Fri 10 Feb 2017
ERR1633254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723883
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27540.3804482216048726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.263164074857401No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17430.2407847677041732No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG17050.2355353005941568No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC15430.21315599344092898No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG14530.2007230450224691No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14310.19768387985351224No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13980.19312513210007695No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG11470.1584510203997055No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT11330.1565170062012784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10960.1514056829625782No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA10890.15043867586336468No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10720.1480902300509889No Hit
CCTTTAATAGCGACTGCACCATCGGGATGTCCTGATCCAACAT10500.14505106488203204No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG10120.13980159777201565No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10020.13842015905885344No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9990.13800572744490477No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9800.13538099388989658No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT9780.13510470614726414No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9640.13317069194883704No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9450.13054595839382882No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9430.1302696706511964No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9130.12612535451170975No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8870.12253361385748801No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8640.11935630481721493No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG8370.11562642029167697No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG8340.11521198867772832No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA7840.10830479511191725No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7730.10678521252743883No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7590.10485119832901173No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7540.10416047897243064No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7430.1026408963879522No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7360.10167388928873865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAC303.5981732E-430.83333433
ATTAGAC451.3225507E-424.6666683
GGTATCA5450.022.064221
GTATCAA15950.021.921631
GCGTGCA450.003824459420.5555579
GACTGCG450.003824459420.5555577
TAGGACC2700.020.5555554
CCGCCGT555.1405653E-420.18181824
CGCCTAT656.897619E-519.92307734
GGACCGT656.897619E-519.9230776
CGGAGTT656.897619E-519.92307724
TCTATCG656.897619E-519.92307729
GACGAAC759.258001E-619.73333420
TTAACGG4350.019.13793235
AACGGCC4400.018.92045437
TTTAACG4600.018.90217434
AACCGGG1501.2732926E-1118.533
CTATCGC609.232312E-418.530
GACCGTT500.007032426518.57
GCACGGA701.2184061E-418.521
Produced by FastQC (version 0.11.5)