##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723883
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833147069346843	33.0	33.0	33.0	27.0	33.0
2	31.96224666140799	33.0	33.0	33.0	33.0	33.0
3	31.68417271851943	33.0	33.0	33.0	27.0	33.0
4	31.962746742222155	33.0	33.0	33.0	33.0	33.0
5	32.059975161731934	33.0	33.0	33.0	33.0	33.0
6	35.23767514916084	37.0	37.0	37.0	33.0	37.0
7	35.36180294329332	37.0	37.0	37.0	33.0	37.0
8	35.48743236130701	37.0	37.0	37.0	33.0	37.0
9	35.600363594669304	37.0	37.0	37.0	33.0	37.0
10	35.61005991299699	37.0	37.0	37.0	33.0	37.0
11	35.631762315180765	37.0	37.0	37.0	33.0	37.0
12	35.61174941254319	37.0	37.0	37.0	33.0	37.0
13	35.61451781572436	37.0	37.0	37.0	33.0	37.0
14	35.58937563114481	37.0	37.0	37.0	33.0	37.0
15	35.59959413330607	37.0	37.0	37.0	33.0	37.0
16	35.59070043087073	37.0	37.0	37.0	33.0	37.0
17	35.58871530343992	37.0	37.0	37.0	33.0	37.0
18	35.57003687059925	37.0	37.0	37.0	33.0	37.0
19	35.55992059490277	37.0	37.0	37.0	33.0	37.0
20	35.549434093631156	37.0	37.0	37.0	33.0	37.0
21	35.56149681647449	37.0	37.0	37.0	33.0	37.0
22	35.464134397409524	37.0	37.0	37.0	33.0	37.0
23	35.51636245083805	37.0	37.0	37.0	33.0	37.0
24	35.490148269817084	37.0	37.0	37.0	33.0	37.0
25	35.518862854908875	37.0	37.0	37.0	33.0	37.0
26	35.41066028626173	37.0	37.0	37.0	33.0	37.0
27	35.421141261778494	37.0	37.0	37.0	33.0	37.0
28	35.45447952224323	37.0	37.0	37.0	33.0	37.0
29	35.46000251421846	37.0	37.0	37.0	33.0	37.0
30	35.43942322170848	37.0	37.0	37.0	33.0	37.0
31	35.43976858138677	37.0	37.0	37.0	33.0	37.0
32	35.427471013962204	37.0	37.0	37.0	33.0	37.0
33	35.44179100766284	37.0	37.0	37.0	33.0	37.0
34	35.40708788574949	37.0	37.0	37.0	33.0	37.0
35	35.354655379391424	37.0	37.0	37.0	33.0	37.0
36	35.39008790094532	37.0	37.0	37.0	33.0	37.0
37	35.37840921806424	37.0	37.0	37.0	33.0	37.0
38	35.36745717194629	37.0	37.0	37.0	33.0	37.0
39	35.2722843332417	37.0	37.0	37.0	33.0	37.0
40	35.058371311385955	37.0	37.0	37.0	33.0	37.0
41	35.24019627481237	37.0	37.0	37.0	33.0	37.0
42	35.291855175491065	37.0	37.0	37.0	33.0	37.0
43	34.95639488701904	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	30.0
16	40.0
17	32.0
18	28.0
19	20.0
20	63.0
21	127.0
22	350.0
23	791.0
24	1683.0
25	3029.0
26	4902.0
27	7285.0
28	10471.0
29	13661.0
30	17850.0
31	22583.0
32	29412.0
33	38523.0
34	55664.0
35	114400.0
36	402937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12816988380719	21.80877296469181	13.573187932303977	24.489869219197026
2	16.017091159759243	21.449184467655684	39.099136186372654	23.434588186212412
3	16.75671344678629	28.401412935515825	29.867948273408825	24.973925344289064
4	11.828845269194055	18.38335753153479	40.85121490627629	28.93658229299486
5	12.826105876225855	38.12314973552356	36.382951388553124	12.667792999697463
6	28.404590244556093	41.36248537401762	17.363303185735816	12.869621195690465
7	26.034179556641057	32.434937690206844	24.48310016950253	17.047782583649568
8	23.52341469546874	36.93069183832194	20.51270716400302	19.033186302206296
9	25.34442720715917	15.761939429438183	20.938190287656983	37.95544307574566
10	15.038341831483818	28.180382741409872	34.2349523334572	22.54632309364911
11	33.017075963933394	24.30434200001934	24.515011403776576	18.163570632270684
12	22.61843419447618	27.04428754370527	29.780917634479604	20.55636062733895
13	26.359646517462075	23.26370421739425	26.38009181041688	23.9965574547268
14	21.278024211094888	21.440066972148813	28.05674397658185	29.22516484017445
15	23.534742492916674	29.307636731350232	25.301878894793774	21.855741880939323
16	22.576991033081313	29.027895391934884	25.556616193500886	22.83849738148292
17	21.826179092477652	28.335794596640618	26.535918097261575	23.30210821362016
18	21.666070345622153	28.695244949805428	28.327091532747694	21.31159317182473
19	24.247703012779688	26.745896781662232	27.863618844481774	21.142781361076306
20	23.093510967932662	27.086973999941982	28.472557029243678	21.34695800288168
21	21.927991125637707	27.949129900826513	28.274596861647534	21.84828211188825
22	21.77478957234802	27.80670356949949	27.92661244980197	22.49189440835052
23	21.922050939171108	27.513700418437786	28.814739398493955	21.74950924389715
24	22.241301425782893	28.706572747253357	26.282838525010256	22.769287301953494
25	22.227487038651272	27.869006455463108	28.079261427606394	21.824245078279226
26	22.144738859732858	28.161457031039543	28.26161133774381	21.43219277148379
27	22.061576249200492	28.314934872071866	26.88707981814741	22.736409060580232
28	21.396966084298153	27.49974788743485	29.03494072937201	22.068345298894986
29	23.93328756166397	27.471980969300287	27.160190251739575	21.434541217296164
30	22.71237202697121	26.824915076055106	28.258019597089586	22.2046932998841
31	22.159382110092377	27.49159739902719	28.14239317679791	22.206627314082525
32	21.004361202017453	27.349585499314117	28.575197925631628	23.070855373036803
33	20.987231361974242	27.448358367305215	29.192701030415137	22.37170924030541
34	22.44326776564721	27.005054684251462	28.78849206294387	21.763185487157454
35	22.258983841311373	28.117527279960985	27.610953703844405	22.012535174883233
36	23.376844048002233	27.707101838280497	27.216691095107908	21.69936301860936
37	21.928681844994287	26.767032793973613	27.840134386358017	23.464150974674084
38	22.358861860273	27.589403259919077	27.634714449710795	22.41702043009713
39	21.7030929031349	27.580285764412203	28.285510227481513	22.431111104971382
40	21.948574562463826	28.542319684258366	28.043344021064176	21.465761732213632
41	21.858366614494333	26.83182226962092	28.178586871082757	23.13122424480199
42	21.450151474754897	28.29849575138524	27.968608186682104	22.282744587177763
43	22.748013145770795	26.51270992688045	27.738322353197965	23.000954574150796
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	45.5
2	59.0
3	188.5
4	318.0
5	318.0
6	378.5
7	439.0
8	388.0
9	337.0
10	500.5
11	664.0
12	664.0
13	1002.5
14	1341.0
15	2522.0
16	3703.0
17	4335.0
18	4967.0
19	4967.0
20	4802.0
21	4637.0
22	5204.5
23	5772.0
24	7639.5
25	9507.0
26	9507.0
27	12539.5
28	15572.0
29	18478.5
30	21385.0
31	26313.0
32	31241.0
33	31241.0
34	38056.5
35	44872.0
36	49108.0
37	53344.0
38	56836.0
39	60328.0
40	60328.0
41	61717.0
42	63106.0
43	65325.0
44	67544.0
45	63440.5
46	59337.0
47	59337.0
48	56400.0
49	53463.0
50	50913.0
51	48363.0
52	45182.5
53	42002.0
54	42002.0
55	41477.5
56	40953.0
57	32944.5
58	24936.0
59	22982.0
60	21028.0
61	21028.0
62	18702.5
63	16377.0
64	13444.0
65	10511.0
66	8563.5
67	6616.0
68	6616.0
69	5467.0
70	4318.0
71	3541.0
72	2764.0
73	2223.0
74	1682.0
75	1682.0
76	1310.5
77	939.0
78	738.5
79	538.0
80	439.5
81	341.0
82	341.0
83	284.5
84	228.0
85	207.0
86	186.0
87	131.0
88	76.0
89	76.0
90	58.5
91	41.0
92	27.0
93	13.0
94	7.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	723883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63794291420585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47281319661705	42.92332565084464
2	7.403877959870153	7.3502654303156305
3	1.9553529879876543	2.911790999845591
4	0.8954965226349002	1.778024210816841
5	0.5618035943955668	1.3943387373801404
6	0.3856752189524084	1.1486474701070095
7	0.2727292733525126	0.9476404071193404
8	0.2151757515096129	0.8544705335964409
9	0.1740949501287138	0.7777543676526548
>10	1.3481549041759597	13.60287898921147
>50	0.1609486087264266	5.600694328519067
>100	0.13577385145505041	12.911544392035887
>500	0.01357738514550429	4.634834167566626
>1k	0.00452579504850143	3.1637903149887543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2754	0.3804482216048726	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1905	0.263164074857401	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1743	0.2407847677041732	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1705	0.2355353005941568	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1543	0.21315599344092898	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1453	0.2007230450224691	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1431	0.19768387985351224	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1398	0.19312513210007695	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1147	0.1584510203997055	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	1133	0.1565170062012784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1096	0.1514056829625782	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1089	0.15043867586336468	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1072	0.1480902300509889	No Hit
CCTTTAATAGCGACTGCACCATCGGGATGTCCTGATCCAACAT	1050	0.14505106488203204	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	1012	0.13980159777201565	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1002	0.13842015905885344	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	999	0.13800572744490477	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	980	0.13538099388989658	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	978	0.13510470614726414	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	964	0.13317069194883704	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	945	0.13054595839382882	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	943	0.1302696706511964	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	913	0.12612535451170975	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	887	0.12253361385748801	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	864	0.11935630481721493	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	837	0.11562642029167697	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	834	0.11521198867772832	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	784	0.10830479511191725	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	773	0.10678521252743883	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	759	0.10485119832901173	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	754	0.10416047897243064	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	743	0.1026408963879522	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	736	0.10167388928873865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	1.38143871316221E-4	1.38143871316221E-4	0.0
3	0.0	0.0	1.38143871316221E-4	1.38143871316221E-4	0.0
4	0.0	0.0	1.38143871316221E-4	1.38143871316221E-4	0.0
5	0.0	0.0	2.76287742632442E-4	1.38143871316221E-4	0.0
6	0.0	0.0	6.907193565811049E-4	1.38143871316221E-4	0.0
7	0.0	0.0	0.0013814387131622099	2.76287742632442E-4	0.0
8	0.0	0.0	0.0016577264557946518	2.76287742632442E-4	0.0
9	0.0	0.0	0.0016577264557946518	8.288632278973259E-4	0.0
10	1.38143871316221E-4	0.0	0.001934014198427094	9.67007099213547E-4	0.0
11	1.38143871316221E-4	0.0	0.0027628774263244197	9.67007099213547E-4	0.0
12	1.38143871316221E-4	0.0	0.002901021297640641	9.67007099213547E-4	0.0
13	1.38143871316221E-4	0.0	0.0033154529115893036	0.001105150970529768	0.0
14	1.38143871316221E-4	0.0	0.0033154529115893036	0.001105150970529768	0.0
15	2.76287742632442E-4	0.0	0.0033154529115893036	0.001105150970529768	0.0
16	2.76287742632442E-4	0.0	0.003453596782905525	0.001519582584478431	0.0
17	2.76287742632442E-4	0.0	0.0035917406542217457	0.001519582584478431	0.0
18	2.76287742632442E-4	0.0	0.0037298845255379666	0.0017958703271108729	0.0
19	2.76287742632442E-4	0.0	0.0037298845255379666	0.0017958703271108729	0.0
20	2.76287742632442E-4	0.0	0.0037298845255379666	0.002072158069743315	0.0
21	2.76287742632442E-4	0.0	0.003868028396854188	0.002072158069743315	1.38143871316221E-4
22	2.76287742632442E-4	0.0	0.003868028396854188	0.0023484458123757567	1.38143871316221E-4
23	2.76287742632442E-4	0.0	0.003868028396854188	0.002486589683691978	1.38143871316221E-4
24	2.76287742632442E-4	0.0	0.004006172268170409	0.0031773090402730827	1.38143871316221E-4
25	2.76287742632442E-4	0.0	0.004006172268170409	0.0035917406542217457	1.38143871316221E-4
26	2.76287742632442E-4	0.0	0.004006172268170409	0.004282460010802851	1.38143871316221E-4
27	2.76287742632442E-4	0.0	0.004006172268170409	0.004973179367383956	1.38143871316221E-4
28	2.76287742632442E-4	0.0	0.004006172268170409	0.011051509705297679	1.38143871316221E-4
29	2.76287742632442E-4	0.0	0.004006172268170409	0.020030861340852045	1.38143871316221E-4
30	2.76287742632442E-4	0.0	0.004006172268170409	0.03771327686932833	1.38143871316221E-4
31	2.76287742632442E-4	0.0	0.004006172268170409	0.0809523085913055	1.38143871316221E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGAAC	30	3.5981732E-4	30.833334	33
ATTAGAC	45	1.3225507E-4	24.666668	3
GGTATCA	545	0.0	22.06422	1
GTATCAA	1595	0.0	21.92163	1
GCGTGCA	45	0.0038244594	20.555557	9
GACTGCG	45	0.0038244594	20.555557	7
TAGGACC	270	0.0	20.555555	4
CCGCCGT	55	5.1405653E-4	20.181818	24
CGCCTAT	65	6.897619E-5	19.923077	34
GGACCGT	65	6.897619E-5	19.923077	6
CGGAGTT	65	6.897619E-5	19.923077	24
TCTATCG	65	6.897619E-5	19.923077	29
GACGAAC	75	9.258001E-6	19.733334	20
TTAACGG	435	0.0	19.137932	35
AACGGCC	440	0.0	18.920454	37
TTTAACG	460	0.0	18.902174	34
AACCGGG	150	1.2732926E-11	18.5	33
CTATCGC	60	9.232312E-4	18.5	30
GACCGTT	50	0.0070324265	18.5	7
GCACGGA	70	1.2184061E-4	18.5	21
>>END_MODULE