FastQCFastQC Report
Fri 10 Feb 2017
ERR1633253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676607
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26450.3909211698962618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.2806651423943293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10280.15193457945306507No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7000.10345739846025832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGT358.86561E-426.428577
GGTATCA4850.024.7938141
ACGTGAT603.7233716E-521.58333237
GTATCAA11350.020.8634361
TTAACGG1500.019.73333435
GTTAGAG609.231465E-418.53
ACGGGTT609.231465E-418.55
AACGGCC1601.8189894E-1218.537
AACGATT701.2182571E-418.522
AGGTCGC1501.2732926E-1118.535
ATCTCGT1002.8705836E-718.49999837
ACCGATC1002.8705836E-718.49999827
GTTTCGG852.7204353E-517.41176421
GCGAACC1401.8644641E-917.17857230
TTTAACG1851.8189894E-1217.00000234
CGATCAG1201.0392978E-716.95833229
TATCTCG1107.795934E-716.81818236
GGCGAAC1452.9722287E-916.58620829
TAACGGC1801.0913936E-1116.44444536
GTCGCCC1708.54925E-1116.3235337
Produced by FastQC (version 0.11.5)