FastQCFastQC Report
Fri 10 Feb 2017
ERR1633252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650459
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59130.9090503782713437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.6466203096582567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23030.3540576731200583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.17095620169757048No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9160.14082363377245913No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7650.11760925746280702No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6930.10654015087807225No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6700.10300418627461531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.025.0994151
GCCGAGT601.3359149E-624.66666812
CCGAGTT551.9010125E-523.54545413
TTTGCCG551.9010125E-523.5454549
GTATCAA24750.023.321211
CGAGTTC705.0963063E-621.14285714
CCGCTCT1500.019.73333228
GCTTCGC1700.019.58823622
ATCGGGA609.23094E-418.521
AACAACG500.00703168118.4999982
CCGGGCG500.00703168118.49999810
GTCAACC1054.7893445E-717.61904715
GCCGGCA1900.017.52631615
TGCCCGC852.7201728E-517.41176630
GCCGCTC1705.456968E-1217.41176627
CGCTCTC1705.456968E-1217.41176629
TTGCCGA752.0655291E-417.26666610
TCCTATA650.001578937417.0769232
CGGGCGA650.001578937417.07692317
CTGCGTC1107.794788E-716.8181829
Produced by FastQC (version 0.11.5)