##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650459
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.857297692859966	33.0	33.0	33.0	33.0	33.0
2	31.984824562347512	33.0	33.0	33.0	33.0	33.0
3	31.698809609829368	33.0	33.0	33.0	27.0	33.0
4	31.98437103645272	33.0	33.0	33.0	33.0	33.0
5	32.0867587349856	33.0	33.0	33.0	33.0	33.0
6	35.25039856470585	37.0	37.0	37.0	33.0	37.0
7	35.39290101297699	37.0	37.0	37.0	33.0	37.0
8	35.526950968469954	37.0	37.0	37.0	33.0	37.0
9	35.63420753652421	37.0	37.0	37.0	33.0	37.0
10	35.64679710788843	37.0	37.0	37.0	33.0	37.0
11	35.6714504680541	37.0	37.0	37.0	33.0	37.0
12	35.659757186848054	37.0	37.0	37.0	33.0	37.0
13	35.65930827308101	37.0	37.0	37.0	33.0	37.0
14	35.63735761977311	37.0	37.0	37.0	33.0	37.0
15	35.65225171763324	37.0	37.0	37.0	33.0	37.0
16	35.62774133342763	37.0	37.0	37.0	33.0	37.0
17	35.63440893276901	37.0	37.0	37.0	33.0	37.0
18	35.61602806633469	37.0	37.0	37.0	33.0	37.0
19	35.60437321952652	37.0	37.0	37.0	33.0	37.0
20	35.61556685356033	37.0	37.0	37.0	33.0	37.0
21	35.595648611211466	37.0	37.0	37.0	33.0	37.0
22	35.50005611422088	37.0	37.0	37.0	33.0	37.0
23	35.5457576880326	37.0	37.0	37.0	33.0	37.0
24	35.54565468384633	37.0	37.0	37.0	33.0	37.0
25	35.557886046622464	37.0	37.0	37.0	33.0	37.0
26	35.45332603592232	37.0	37.0	37.0	33.0	37.0
27	35.459221872554615	37.0	37.0	37.0	33.0	37.0
28	35.474584870068675	37.0	37.0	37.0	33.0	37.0
29	35.47742440338284	37.0	37.0	37.0	33.0	37.0
30	35.47427585750985	37.0	37.0	37.0	33.0	37.0
31	35.46845996442512	37.0	37.0	37.0	33.0	37.0
32	35.44941956372346	37.0	37.0	37.0	33.0	37.0
33	35.432722123915575	37.0	37.0	37.0	33.0	37.0
34	35.412325757657285	37.0	37.0	37.0	33.0	37.0
35	35.33878076865721	37.0	37.0	37.0	33.0	37.0
36	35.35280009962196	37.0	37.0	37.0	33.0	37.0
37	35.348034234286864	37.0	37.0	37.0	33.0	37.0
38	35.31482230240492	37.0	37.0	37.0	33.0	37.0
39	35.21305262898968	37.0	37.0	37.0	33.0	37.0
40	34.97906094004388	37.0	37.0	37.0	27.0	37.0
41	35.136040857302305	37.0	37.0	37.0	33.0	37.0
42	35.16445463895495	37.0	37.0	37.0	33.0	37.0
43	34.81881409896704	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	29.0
16	34.0
17	39.0
18	24.0
19	26.0
20	52.0
21	98.0
22	315.0
23	668.0
24	1432.0
25	2570.0
26	4237.0
27	6507.0
28	8963.0
29	12362.0
30	16342.0
31	20799.0
32	26396.0
33	34669.0
34	49595.0
35	100648.0
36	364644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81846972676218	20.882177047285072	14.193361918276171	23.10599130767658
2	15.514275304054523	22.768383556842167	39.77314481004952	21.944196329053792
3	17.702422443228553	28.376269680333426	30.52413757054634	23.397170305891684
4	11.69235878049193	18.437595605564685	40.9546182003785	28.91542741356488
5	13.321362299545397	37.37929677350917	35.259409124941	14.03993180200443
6	27.505653699925748	41.537898622357446	17.619865356617403	13.336582321099408
7	25.37362078163266	33.23929717322691	23.762143348005026	17.62493869713541
8	24.122965475149087	35.432363915327485	20.98933214852896	19.45533846099447
9	26.27144831572782	15.053984955239299	20.85112205381123	37.82344467522165
10	14.80031792933913	28.240980599853334	34.03781022324235	22.92089124756518
11	33.01161180028257	24.778348827520258	24.213824391698786	17.996214980498387
12	22.74071079038033	26.415654176512277	30.879732619580942	19.963902413526448
13	28.146431981108726	23.2669545659296	26.573081470161842	22.01353198279984
14	22.004153989721104	21.881163916557387	29.225208660346002	26.88947343337551
15	23.450363512534995	29.01889281261386	26.17028898055066	21.360454694300486
16	21.494975086823302	29.342356705034444	26.18474031414739	22.977927893994856
17	21.525107654748414	27.569762275562333	27.257060014543576	23.648070055145674
18	22.578978844170038	26.543717590194	29.340358116345534	21.536945449290425
19	24.56911196555048	26.326640111060033	29.212755915438176	19.89149200795131
20	24.10297958825998	26.16936655500193	29.05548235938007	20.67217149735802
21	22.433235607471033	27.1454465231475	28.980612152341656	21.440705717039812
22	22.1611200705963	26.903771029380792	29.212755915438176	21.72235298458473
23	21.70451942397599	26.85918712785894	29.87797847366244	21.558314974502622
24	21.859640653753733	27.451384330142254	28.71372369357638	21.97525132252763
25	21.909144158202132	27.378666449384205	29.649985625535198	21.062203766878465
26	22.361286414670257	27.13422367897131	28.983994379353657	21.520495527004776
27	22.244907057939088	27.053818918640527	28.87084351204303	21.83043051137735
28	21.607357266176656	27.092253316504188	29.378946251800652	21.921443165518504
29	22.651235512153725	27.20156074402845	28.783059347322432	21.364144396495398
30	22.61433849020461	26.787237935058165	29.149569765350314	21.448853809386907
31	22.3313075843366	26.588608966898757	29.122358211662842	21.957725237101798
32	21.146913179769978	26.921604589989528	29.429679656980685	22.501802573259805
33	21.264676174824242	26.776630041247795	29.906573665673008	22.052120118254955
34	21.849186497534816	26.784624396003437	29.512236743591835	21.85395236286991
35	21.770626588301493	27.425556414777873	28.878069178841404	21.925747818079234
36	22.18956152501541	26.99970328644849	28.804736347717537	22.00599884081856
37	22.243523419615993	26.819061616489282	29.021045138894223	21.9163698250005
38	21.988319017801274	26.95957777507883	28.56844166965174	22.48366153746816
39	21.822589894213163	26.762332445242514	29.087920991177	22.327156669367323
40	21.808446035799335	27.527023225137942	29.25134405089329	21.413186688169432
41	22.095474119045168	26.774938927741793	28.936335726002717	22.19325122721032
42	21.27359295512861	27.889382728196548	28.886217271188496	21.950807045486343
43	22.049506579200226	26.74449888463377	28.70234711180874	22.50364742435726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	71.5
2	91.0
3	348.0
4	605.0
5	605.0
6	771.5
7	938.0
8	870.0
9	802.0
10	1109.0
11	1416.0
12	1416.0
13	2268.5
14	3121.0
15	5391.5
16	7662.0
17	8759.5
18	9857.0
19	9857.0
20	8928.5
21	8000.0
22	8248.0
23	8496.0
24	10613.5
25	12731.0
26	12731.0
27	15047.0
28	17363.0
29	19844.5
30	22326.0
31	25122.0
32	27918.0
33	27918.0
34	32404.0
35	36890.0
36	38802.0
37	40714.0
38	41873.0
39	43032.0
40	43032.0
41	43888.5
42	44745.0
43	45723.0
44	46701.0
45	46525.0
46	46349.0
47	46349.0
48	45570.0
49	44791.0
50	45254.0
51	45717.0
52	44951.5
53	44186.0
54	44186.0
55	41509.5
56	38833.0
57	33702.5
58	28572.0
59	25867.5
60	23163.0
61	23163.0
62	20053.5
63	16944.0
64	13156.5
65	9369.0
66	8072.0
67	6775.0
68	6775.0
69	5618.5
70	4462.0
71	3965.0
72	3468.0
73	3037.0
74	2606.0
75	2606.0
76	1683.5
77	761.0
78	571.0
79	381.0
80	305.5
81	230.0
82	230.0
83	196.5
84	163.0
85	137.0
86	111.0
87	89.5
88	68.0
89	68.0
90	53.5
91	39.0
92	24.0
93	9.0
94	5.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	650459.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.33063124644199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.37515719349427	54.814348084269845
2	6.401732067899111	7.852445375896861
3	1.3868693738384459	2.551727224616089
4	0.6117600807495349	1.500785276949732
5	0.39050661284445126	1.1975008535830065
6	0.2655694469065679	0.9772525091128963
7	0.1913557551485377	0.8215178479129587
8	0.15056933284358986	0.7387609783722398
9	0.11376133531423632	0.6279349055634241
>10	0.9002594247409715	11.127133717821065
>50	0.1302170620918886	5.527355931691089
>100	0.07919630249410334	9.333373941577518
>500	0.0020306744229243753	0.8229148608937161
>1k	7.615029085966408E-4	1.1864234118182009
>5k	2.538343028655469E-4	0.9205250799214044
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5913	0.9090503782713437	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4206	0.6466203096582567	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2303	0.3540576731200583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.17095620169757048	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	916	0.14082363377245913	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	765	0.11760925746280702	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	693	0.10654015087807225	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	670	0.10300418627461531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.5373759145464972E-4	0.0	0.0
2	1.5373759145464972E-4	0.0	1.5373759145464972E-4	1.5373759145464972E-4	0.0
3	1.5373759145464972E-4	0.0	3.0747518290929945E-4	1.5373759145464972E-4	0.0
4	1.5373759145464972E-4	0.0	6.149503658185989E-4	1.5373759145464972E-4	0.0
5	3.0747518290929945E-4	0.0	9.224255487278983E-4	1.5373759145464972E-4	0.0
6	3.0747518290929945E-4	0.0	0.0019985886889104464	1.5373759145464972E-4	1.5373759145464972E-4
7	3.0747518290929945E-4	0.0	0.006764454024004588	4.6121277436394917E-4	1.5373759145464972E-4
8	3.0747518290929945E-4	0.0	0.007840617164187135	4.6121277436394917E-4	1.5373759145464972E-4
9	3.0747518290929945E-4	0.0	0.008916780304369683	9.224255487278983E-4	1.5373759145464972E-4
10	9.224255487278983E-4	0.0	0.010300418627461531	0.001076163140182548	1.5373759145464972E-4
11	9.224255487278983E-4	0.0	0.014143858413827775	0.0015373759145464972	1.5373759145464972E-4
12	9.224255487278983E-4	0.0	0.015066283962555672	0.001691113506001147	1.5373759145464972E-4
13	9.224255487278983E-4	0.0	0.01660365987710217	0.001691113506001147	1.5373759145464972E-4
14	9.224255487278983E-4	0.0	0.018141035791648667	0.001691113506001147	1.5373759145464972E-4
15	9.224255487278983E-4	0.0	0.02013962448055911	0.0018448510974557967	1.5373759145464972E-4
16	9.224255487278983E-4	0.0	0.02152326280365096	0.002152326280365096	1.5373759145464972E-4
17	9.224255487278983E-4	0.0	0.02183073798656026	0.002152326280365096	1.5373759145464972E-4
18	9.224255487278983E-4	0.0	0.022445688352378857	0.002152326280365096	1.5373759145464972E-4
19	9.224255487278983E-4	0.0	0.023675589084016055	0.002306063871819746	1.5373759145464972E-4
20	9.224255487278983E-4	0.0	0.024290539449834656	0.0024598014632743956	1.5373759145464972E-4
21	9.224255487278983E-4	0.0	0.02613539054729045	0.0029210142376383448	1.5373759145464972E-4
22	0.001076163140182548	0.0	0.027211553687472998	0.003228489420547644	1.5373759145464972E-4
23	0.001076163140182548	0.0	0.027365291278927648	0.004612127743639492	1.5373759145464972E-4
24	0.001076163140182548	0.0	0.027365291278927648	0.00553455329236739	1.5373759145464972E-4
25	0.001076163140182548	0.0	0.027365291278927648	0.0058420284752766895	1.5373759145464972E-4
26	0.001076163140182548	0.0	0.027365291278927648	0.006149503658185989	1.5373759145464972E-4
27	0.001076163140182548	0.0	0.027365291278927648	0.007840617164187135	1.5373759145464972E-4
28	0.001076163140182548	0.0	0.027365291278927648	0.017679823017284716	1.5373759145464972E-4
29	0.001076163140182548	0.0	0.027365291278927648	0.040894199326936824	1.5373759145464972E-4
30	0.001076163140182548	0.0	0.027365291278927648	0.07118050484350281	1.5373759145464972E-4
31	0.001076163140182548	0.0	0.027365291278927648	0.1500478892597381	1.5373759145464972E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	855	0.0	25.099415	1
GCCGAGT	60	1.3359149E-6	24.666668	12
CCGAGTT	55	1.9010125E-5	23.545454	13
TTTGCCG	55	1.9010125E-5	23.545454	9
GTATCAA	2475	0.0	23.32121	1
CGAGTTC	70	5.0963063E-6	21.142857	14
CCGCTCT	150	0.0	19.733332	28
GCTTCGC	170	0.0	19.588236	22
ATCGGGA	60	9.23094E-4	18.5	21
AACAACG	50	0.007031681	18.499998	2
CCGGGCG	50	0.007031681	18.499998	10
GTCAACC	105	4.7893445E-7	17.619047	15
GCCGGCA	190	0.0	17.526316	15
TGCCCGC	85	2.7201728E-5	17.411766	30
GCCGCTC	170	5.456968E-12	17.411766	27
CGCTCTC	170	5.456968E-12	17.411766	29
TTGCCGA	75	2.0655291E-4	17.266666	10
TCCTATA	65	0.0015789374	17.076923	2
CGGGCGA	65	0.0015789374	17.076923	17
CTGCGTC	110	7.794788E-7	16.818182	9
>>END_MODULE