FastQCFastQC Report
Fri 10 Feb 2017
ERR1633251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620376
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44990.7252053593304706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.5138819038776484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16910.27257663094639384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.14023753336686137No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7850.12653616516435193No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7610.12266754355423162No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7100.11444672263272596No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6930.11170644899222407No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6320.10187370239983494No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6230.10042296929603982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGA451.322253E-424.6666682
GGTATCA7950.024.4339641
CGTATGC400.001930190523.12500236
GTATCAA21300.022.6690141
GCCGGCA1250.022.215
TTACTCG753.735022E-722.19999919
TGCGGGT753.735022E-722.19999921
TTAACGG2350.021.2553235
AGCTTCG1500.020.96666521
TACTCGC450.003823852220.55555720
GCACGGC555.139428E-420.18181810
TTCGCCG1500.019.73333224
CATACTA951.6730155E-719.4736845
TAACGGC2500.019.24000236
GCCGCTC1457.2759576E-1219.1379327
TTTAACG2650.018.84905634
AACGGCC2650.018.84905637
GTTCTAT500.007031319718.51
CCGGAAT500.007031319718.53
ATCCGTA500.007031319718.512
Produced by FastQC (version 0.11.5)