##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633251.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620376
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.689594697409312	33.0	33.0	33.0	27.0	33.0
2	31.841160844391144	33.0	33.0	33.0	33.0	33.0
3	31.575020632648588	33.0	33.0	33.0	27.0	33.0
4	31.87437940861671	33.0	33.0	33.0	27.0	33.0
5	31.979307710162868	33.0	33.0	33.0	33.0	33.0
6	35.085622267785986	37.0	37.0	37.0	33.0	37.0
7	35.23861819283789	37.0	37.0	37.0	33.0	37.0
8	35.38587405057578	37.0	37.0	37.0	33.0	37.0
9	35.510798290069246	37.0	37.0	37.0	33.0	37.0
10	35.512253858950054	37.0	37.0	37.0	33.0	37.0
11	35.515211742556126	37.0	37.0	37.0	33.0	37.0
12	35.49160992688305	37.0	37.0	37.0	33.0	37.0
13	35.51630462816099	37.0	37.0	37.0	33.0	37.0
14	35.48861658091222	37.0	37.0	37.0	33.0	37.0
15	35.49999838807433	37.0	37.0	37.0	33.0	37.0
16	35.47930932208854	37.0	37.0	37.0	33.0	37.0
17	35.474376829535636	37.0	37.0	37.0	33.0	37.0
18	35.46082859427186	37.0	37.0	37.0	33.0	37.0
19	35.456906779114604	37.0	37.0	37.0	33.0	37.0
20	35.458739538602394	37.0	37.0	37.0	33.0	37.0
21	35.452683533856884	37.0	37.0	37.0	33.0	37.0
22	35.36022831315202	37.0	37.0	37.0	33.0	37.0
23	35.404077527177066	37.0	37.0	37.0	33.0	37.0
24	35.39452203180007	37.0	37.0	37.0	33.0	37.0
25	35.406574400041265	37.0	37.0	37.0	33.0	37.0
26	35.28407288483113	37.0	37.0	37.0	33.0	37.0
27	35.307147600809834	37.0	37.0	37.0	33.0	37.0
28	35.32617799528028	37.0	37.0	37.0	33.0	37.0
29	35.32572504416677	37.0	37.0	37.0	33.0	37.0
30	35.31953524959057	37.0	37.0	37.0	33.0	37.0
31	35.305126246018546	37.0	37.0	37.0	33.0	37.0
32	35.2872306472204	37.0	37.0	37.0	33.0	37.0
33	35.2823529601403	37.0	37.0	37.0	33.0	37.0
34	35.257468051633204	37.0	37.0	37.0	33.0	37.0
35	35.18351451377874	37.0	37.0	37.0	33.0	37.0
36	35.21002746721343	37.0	37.0	37.0	33.0	37.0
37	35.188779063019844	37.0	37.0	37.0	33.0	37.0
38	35.177481075992624	37.0	37.0	37.0	33.0	37.0
39	35.05711858614776	37.0	37.0	37.0	33.0	37.0
40	34.83285136755774	37.0	37.0	37.0	27.0	37.0
41	35.010519426928184	37.0	37.0	37.0	27.0	37.0
42	35.02690948714973	37.0	37.0	37.0	27.0	37.0
43	34.66271583684733	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	13.0
16	39.0
17	18.0
18	20.0
19	27.0
20	53.0
21	162.0
22	344.0
23	833.0
24	1653.0
25	2815.0
26	4787.0
27	6918.0
28	9783.0
29	13084.0
30	16928.0
31	21423.0
32	26992.0
33	35983.0
34	51174.0
35	102090.0
36	325231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68762170038815	20.987272234902704	13.709427830863863	23.61567823384528
2	15.883915560885656	22.314048254606885	39.08129908313668	22.72073710137078
3	17.663320308973912	28.248513804531445	29.996969579738742	24.0911963067559
4	11.906972545681974	18.355319999484184	40.53541723084065	29.202290223993195
5	13.631894206094369	37.371045946329325	35.41110552310212	13.585954324474189
6	28.07087959560009	41.10942396224225	17.406701742169265	13.412994699988396
7	26.219582962590426	32.640688872554705	23.725289179465356	17.414438985389506
8	24.261576850168286	35.47654970533999	20.917959431054715	19.343914013437015
9	26.011322165912286	15.183856242020969	20.717919455298077	38.08690213676867
10	15.013314506041498	28.057661805098842	33.9839065341019	22.94511715475776
11	33.35074213057887	24.379731001844043	24.145518201864675	18.124008665712406
12	22.92964266831728	26.361432421628173	30.25294337627503	20.455981533779514
13	27.718351451377877	22.850013540175635	26.52923388396714	22.90240112447935
14	21.948785897584692	21.531297148825875	28.64665944523966	27.873257508349774
15	23.684023882290738	28.84202483655074	25.79741962938605	21.676531651772475
16	22.326460082272686	29.005796484712498	25.714566649902636	22.953176783112177
17	22.10836653900215	27.56376777954015	26.639167214721393	23.6886984667363
18	22.51908519994326	26.976059679936036	28.8397681406115	21.6650869795092
19	24.721459244071337	26.283254026590324	28.465640192399448	20.529646536938888
20	23.906792010006832	26.38802919519775	28.557519955639805	21.147658839155607
21	22.28696790333604	27.35212193895315	28.63199092163462	21.728919236076187
22	22.412375720530775	26.790365842650267	28.565418391427134	22.231840045391827
23	21.811772215559596	27.022160754123302	29.327698041187926	21.838368989129172
24	22.258275626394315	27.669832488684282	27.63807755296788	22.433814331953524
25	22.46814834874334	27.342127999793675	28.88844829587218	21.301275355590803
26	22.703489496692328	27.40193044218345	28.291552219944034	21.603027841180186
27	22.47604678453067	27.08712135865991	27.925967477787665	22.510864379021754
28	21.798232039924176	26.94301520368293	29.138619159993297	22.1201335963996
29	23.523476085470747	26.984280500857544	27.95546571756483	21.536777696106878
30	22.98219144518808	26.640295562691012	28.429049479670393	21.948463512450513
31	22.46363495686487	26.680110126761836	28.6419848607941	22.214270055579195
32	21.375262743884356	26.848717551936247	28.829774201452025	22.94624550272738
33	21.499542213109468	26.87740982887797	29.358969399203065	22.264078558809498
34	22.419145808348485	26.728629089455424	28.96646549834294	21.885759603853145
35	22.16849136652611	27.595361522689466	28.127296994081007	22.10885011670342
36	22.844371800327544	27.022321946690393	27.934188298709167	22.199117954272893
37	22.398674352328264	26.726050008382014	28.450971668794406	22.424303970495313
38	22.410119024591538	26.836305724270442	27.97013424116987	22.783441009968147
39	21.90107289772654	26.973964176563893	28.558648303609424	22.566314622100144
40	22.258598011528495	27.75590931950946	28.45403432756909	21.531458341392963
41	22.17977484622229	26.696874153739024	28.29413130101745	22.829219699021237
42	21.524527061008165	28.04782905850645	28.142449095387313	22.28519478509807
43	22.74427121616568	26.466368782802686	27.985125149909084	22.804234851122544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	42.5
2	61.0
3	268.0
4	475.0
5	475.0
6	599.5
7	724.0
8	694.5
9	665.0
10	875.0
11	1085.0
12	1085.0
13	1756.0
14	2427.0
15	4259.0
16	6091.0
17	6783.5
18	7476.0
19	7476.0
20	6702.5
21	5929.0
22	6112.0
23	6295.0
24	7757.5
25	9220.0
26	9220.0
27	11574.5
28	13929.0
29	15980.0
30	18031.0
31	21227.0
32	24423.0
33	24423.0
34	29829.5
35	35236.0
36	38075.5
37	40915.0
38	42780.0
39	44645.0
40	44645.0
41	45822.5
42	47000.0
43	48139.5
44	49279.0
45	48135.5
46	46992.0
47	46992.0
48	45293.5
49	43595.0
50	44139.0
51	44683.0
52	43292.5
53	41902.0
54	41902.0
55	39713.0
56	37524.0
57	31742.5
58	25961.0
59	23587.5
60	21214.0
61	21214.0
62	18703.0
63	16192.0
64	12699.0
65	9206.0
66	7870.0
67	6534.0
68	6534.0
69	5470.5
70	4407.0
71	4057.0
72	3707.0
73	3170.5
74	2634.0
75	2634.0
76	1729.5
77	825.0
78	620.5
79	416.0
80	336.5
81	257.0
82	257.0
83	214.5
84	172.0
85	141.5
86	111.0
87	91.0
88	71.0
89	71.0
90	53.0
91	35.0
92	20.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.77332437291188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.8759062103072	49.01131421631046
2	6.829546545107999	7.618130295604161
3	1.7071790295830818	2.8564514933871044
4	0.792556655393069	1.768140777005911
5	0.46993706233865623	1.3104976106333595
6	0.3338391154778449	1.11715903655471
7	0.2485267231212449	0.9702813080784637
8	0.18717536533124485	0.8351513892190243
9	0.14964214468542758	0.7511435887858707
>10	1.1274782111145858	12.701357247852348
>50	0.1649321452405489	6.5118436604821435
>100	0.10682437408512818	11.132692335116849
>500	0.005575997548399496	1.8808463959836368
>1k	8.804206655367626E-4	1.5349906449860364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4499	0.7252053593304706	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3188	0.5138819038776484	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1691	0.27257663094639384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.14023753336686137	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	785	0.12653616516435193	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	761	0.12266754355423162	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	710	0.11444672263272596	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	693	0.11170644899222407	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	632	0.10187370239983494	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	623	0.10042296929603982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.8357770126503926E-4	0.0	0.0
2	0.0	0.0	8.059628354417321E-4	0.0	0.0
3	0.0	0.0	9.671554025300785E-4	0.0	0.0
4	0.0	0.0	9.671554025300785E-4	1.611925670883464E-4	0.0
5	0.0	0.0	0.0011283479696184249	1.611925670883464E-4	0.0
6	0.0	0.0	0.0025790810734135426	4.8357770126503926E-4	0.0
7	0.0	0.0	0.0069312803847988965	4.8357770126503926E-4	0.0
8	0.0	0.0	0.007737243220240628	4.8357770126503926E-4	0.0
9	0.0	0.0	0.008382013488594013	0.0012895405367067713	0.0
10	8.059628354417321E-4	0.0	0.008865591189859053	0.0014507331037951177	0.0
11	8.059628354417321E-4	0.0	0.012895405367067713	0.0014507331037951177	0.0
12	8.059628354417321E-4	0.0	0.01482971617212787	0.0016119256708834642	0.0
13	8.059628354417321E-4	0.0	0.015635679007569604	0.0017731182379718106	0.0
14	8.059628354417321E-4	0.0	0.016764026977188026	0.0017731182379718106	0.0
15	8.059628354417321E-4	0.0	0.018214760080983147	0.0017731182379718106	0.0
16	8.059628354417321E-4	0.0	0.01950430061768992	0.0017731182379718106	1.611925670883464E-4
17	8.059628354417321E-4	0.0	0.019987878318954957	0.0017731182379718106	1.611925670883464E-4
18	8.059628354417321E-4	0.0	0.02031026345313165	0.001934310805060157	1.611925670883464E-4
19	8.059628354417321E-4	0.0	0.02063264858730834	0.0022566959392368497	1.611925670883464E-4
20	8.059628354417321E-4	0.0	0.020955033721485036	0.002417888506325196	1.611925670883464E-4
21	8.059628354417321E-4	0.0	0.02159980398983842	0.0029014662075902355	1.611925670883464E-4
22	8.059628354417321E-4	1.611925670883464E-4	0.021922189124015112	0.003385043908855275	1.611925670883464E-4
23	8.059628354417321E-4	1.611925670883464E-4	0.02208338169110346	0.0040298141772086606	1.611925670883464E-4
24	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.004996969579738739	1.611925670883464E-4
25	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.005641739848092125	1.611925670883464E-4
26	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.006447702683533857	1.611925670883464E-4
27	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.009187976324035747	1.611925670883464E-4
28	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.020149070886043303	1.611925670883464E-4
29	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.04416676338220692	1.611925670883464E-4
30	8.059628354417321E-4	1.611925670883464E-4	0.0225669593923685	0.07914555044037809	1.611925670883464E-4
31	0.0011283479696184249	1.611925670883464E-4	0.0225669593923685	0.15458367183772423	1.611925670883464E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGGA	45	1.322253E-4	24.666668	2
GGTATCA	795	0.0	24.433964	1
CGTATGC	40	0.0019301905	23.125002	36
GTATCAA	2130	0.0	22.669014	1
GCCGGCA	125	0.0	22.2	15
TTACTCG	75	3.735022E-7	22.199999	19
TGCGGGT	75	3.735022E-7	22.199999	21
TTAACGG	235	0.0	21.25532	35
AGCTTCG	150	0.0	20.966665	21
TACTCGC	45	0.0038238522	20.555557	20
GCACGGC	55	5.139428E-4	20.181818	10
TTCGCCG	150	0.0	19.733332	24
CATACTA	95	1.6730155E-7	19.473684	5
TAACGGC	250	0.0	19.240002	36
GCCGCTC	145	7.2759576E-12	19.13793	27
TTTAACG	265	0.0	18.849056	34
AACGGCC	265	0.0	18.849056	37
GTTCTAT	50	0.0070313197	18.5	1
CCGGAAT	50	0.0070313197	18.5	3
ATCCGTA	50	0.0070313197	18.5	12
>>END_MODULE