FastQCFastQC Report
Fri 10 Feb 2017
ERR1633250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851864
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38060.4467849328061756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.3305691988392513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15500.1819539269179118No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12510.14685442746729527No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12430.14591531042513828No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12060.14157189410516233No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11070.1299503207084699No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10480.12302433252256229No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9400.11034625245344327No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8880.10424199167942301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.026.51
CTGTCGA400.001930778623.1250029
GTATCAA21600.022.611111
GTCTATA555.1415915E-420.1818181
CGTAGCG656.899449E-519.92307715
CATAATA2150.019.7906972
GACCGTT609.2341425E-418.57
AAGGTCG701.21872756E-418.520
CTTAGTG609.2341425E-418.53
TTTACCG500.007033425418.49999830
TCGTATG500.007033425418.49999837
TTAACGG2700.017.81481435
AACGGCC2750.017.4909137
GGTCGGC752.066475E-417.26666622
CGTCCTA752.066475E-417.26666633
GTCGGCT752.066475E-417.26666623
TAACGGC2800.017.17857236
GCAACGC650.0015794817.0769233
GCGGAAT650.0015794817.07692319
CCGTTTA650.0015794817.07692327
Produced by FastQC (version 0.11.5)