##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851864
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606629696759107	33.0	33.0	33.0	27.0	33.0
2	31.7880530225482	33.0	33.0	33.0	27.0	33.0
3	31.50316599832837	33.0	33.0	33.0	27.0	33.0
4	31.808686597860692	33.0	33.0	33.0	27.0	33.0
5	31.921337208756327	33.0	33.0	33.0	33.0	33.0
6	34.98650723589681	37.0	37.0	37.0	27.0	37.0
7	35.14260844454044	37.0	37.0	37.0	33.0	37.0
8	35.28058586816675	37.0	37.0	37.0	33.0	37.0
9	35.40367359108966	37.0	37.0	37.0	33.0	37.0
10	35.41306710930383	37.0	37.0	37.0	33.0	37.0
11	35.423587567968596	37.0	37.0	37.0	33.0	37.0
12	35.41668740550135	37.0	37.0	37.0	33.0	37.0
13	35.42785820271781	37.0	37.0	37.0	33.0	37.0
14	35.391582459171886	37.0	37.0	37.0	33.0	37.0
15	35.419315759323084	37.0	37.0	37.0	33.0	37.0
16	35.38951640167914	37.0	37.0	37.0	33.0	37.0
17	35.38824272419072	37.0	37.0	37.0	33.0	37.0
18	35.3642905440305	37.0	37.0	37.0	33.0	37.0
19	35.35496041621667	37.0	37.0	37.0	33.0	37.0
20	35.354277208568504	37.0	37.0	37.0	33.0	37.0
21	35.35222289003879	37.0	37.0	37.0	33.0	37.0
22	35.24618953260145	37.0	37.0	37.0	33.0	37.0
23	35.29007447198144	37.0	37.0	37.0	33.0	37.0
24	35.29394011250622	37.0	37.0	37.0	33.0	37.0
25	35.30927706770095	37.0	37.0	37.0	33.0	37.0
26	35.194499356704824	37.0	37.0	37.0	33.0	37.0
27	35.216792821389326	37.0	37.0	37.0	33.0	37.0
28	35.23048162617507	37.0	37.0	37.0	33.0	37.0
29	35.22500422602669	37.0	37.0	37.0	33.0	37.0
30	35.22639177145648	37.0	37.0	37.0	33.0	37.0
31	35.213956687922014	37.0	37.0	37.0	33.0	37.0
32	35.203977395452796	37.0	37.0	37.0	33.0	37.0
33	35.21203971525971	37.0	37.0	37.0	33.0	37.0
34	35.18078120451152	37.0	37.0	37.0	33.0	37.0
35	35.11528131250998	37.0	37.0	37.0	33.0	37.0
36	35.1420238441817	37.0	37.0	37.0	33.0	37.0
37	35.13648422752928	37.0	37.0	37.0	33.0	37.0
38	35.12805330428331	37.0	37.0	37.0	33.0	37.0
39	35.02832494388775	37.0	37.0	37.0	27.0	37.0
40	34.823463604519034	37.0	37.0	37.0	27.0	37.0
41	34.99202689631209	37.0	37.0	37.0	27.0	37.0
42	35.03157194106102	37.0	37.0	37.0	27.0	37.0
43	34.66183921377121	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	33.0
16	40.0
17	42.0
18	31.0
19	46.0
20	98.0
21	208.0
22	557.0
23	1361.0
24	2540.0
25	4451.0
26	7045.0
27	10265.0
28	14274.0
29	18719.0
30	23927.0
31	30495.0
32	38405.0
33	50621.0
34	72647.0
35	143535.0
36	432517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51918616117127	20.16519068771541	13.42819980654189	24.88742334457143
2	16.53327291680362	21.360451903120687	38.43148671618944	23.674788463886255
3	18.009799686334908	27.162199599936137	29.69077223594376	25.137228477785182
4	12.689819032145977	17.966600302395687	39.31848276250669	30.025097902951646
5	14.07736446193289	36.964351117079715	35.033526478405	13.924757942582383
6	29.461040729506117	39.78393264652574	17.169994271386045	13.585032352582102
7	26.955241681770797	31.960970295727957	23.156748025506417	17.927039996994825
8	24.806541889315664	35.21254566456618	20.405722040137864	19.575190405980297
9	26.532169454279085	15.117201806861189	19.835208437027507	38.515420301832215
10	15.751340589577678	27.42562193026117	33.29686428819624	23.526173191964915
11	34.191490660481016	23.62701088436652	23.669036372003045	18.512462083149423
12	23.628536949560026	25.68579022003512	29.397650329160523	21.28802250124433
13	28.08441253533428	21.997525426593917	25.795079965816143	24.122982072255667
14	22.357442033000574	20.834781138773327	27.58879351633594	29.218983311890163
15	24.533258830047988	28.013039640130348	24.589605852577407	22.864095677244258
16	23.119418123080678	27.730365410441106	24.88131908379741	24.268897382680805
17	22.656433421297294	27.099748316632706	25.86351812026333	24.380300141806675
18	23.38495346675056	26.601311946507895	27.406956979048296	22.606777607693246
19	25.5297794014068	25.53200980438192	27.034597071833062	21.90361372237822
20	24.738338514129016	25.628269301203012	27.213616257994232	22.41977592667374
21	23.83103406177512	26.445301127879567	27.061714076425346	22.66195073391997
22	23.347154005803745	26.374515180826986	26.93833757501197	23.339993238357295
23	23.294563451442954	26.331785355408844	27.51507282852662	22.858578364621582
24	23.538968661664303	26.811791553581322	26.095362640045828	23.553877144708547
25	23.42780068179897	26.43896208784501	27.551463613910204	22.58177361644582
26	23.665632072725224	26.665289295004836	27.290271686560295	22.378806945709645
27	23.53403829719298	26.61974211846022	26.441544659710942	23.404674924635856
28	22.77335349304584	26.263581980222195	27.720739460758992	23.24232506597297
29	24.392156494463904	26.096653925978796	26.590042542002006	22.92114703755529
30	23.906750373299023	25.984898997962112	27.287689114694363	22.820661514044495
31	23.519129814148737	26.065663063587614	27.225942169172544	23.189264953091104
32	22.328212015063436	26.09219312002855	27.626710366913027	23.952884497994987
33	22.724402017223408	26.153235727768752	27.841650779936707	23.28071147507114
34	23.34879746062752	26.097827822281488	27.53080303898275	23.022571678108243
35	23.43554839739677	26.609529220626765	26.99468459754139	22.960237784435076
36	24.322192274823212	26.329437562803452	26.514913178629453	22.833456983743883
37	23.464308856812824	25.91164786867387	26.913216194134275	23.710827080379026
38	23.8210559432022	26.294338063352836	26.570908032268058	23.313697961176903
39	22.931829493909824	26.30736831231276	27.19952950236188	23.56127269141553
40	23.229999154794662	26.675267413577753	27.439239127372446	22.65549430425514
41	23.08924898810139	25.73885033291699	27.359179399528564	23.812721279453058
42	22.589521332043613	26.971324061117734	27.058544556408066	23.380610050430587
43	23.591441826394824	25.59680888029075	27.173469004442026	23.6382802888724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	36.0
2	47.0
3	191.0
4	335.0
5	335.0
6	449.5
7	564.0
8	540.0
9	516.0
10	687.0
11	858.0
12	858.0
13	1421.0
14	1984.0
15	3533.5
16	5083.0
17	5634.0
18	6185.0
19	6185.0
20	5574.5
21	4964.0
22	5192.5
23	5421.0
24	7130.5
25	8840.0
26	8840.0
27	11419.5
28	13999.0
29	17265.0
30	20531.0
31	24750.0
32	28969.0
33	28969.0
34	35983.0
35	42997.0
36	47058.0
37	51119.0
38	54940.0
39	58761.0
40	58761.0
41	62015.5
42	65270.0
43	67274.5
44	69279.0
45	68319.5
46	67360.0
47	67360.0
48	65162.0
49	62964.0
50	63849.5
51	64735.0
52	61986.5
53	59238.0
54	59238.0
55	57829.0
56	56420.0
57	49062.0
58	41704.0
59	37579.0
60	33454.0
61	33454.0
62	30444.0
63	27434.0
64	22001.5
65	16569.0
66	13854.0
67	11139.0
68	11139.0
69	9359.5
70	7580.0
71	6546.5
72	5513.0
73	5432.0
74	5351.0
75	5351.0
76	4474.0
77	3597.0
78	2685.5
79	1774.0
80	1174.5
81	575.0
82	575.0
83	454.5
84	334.0
85	264.0
86	194.0
87	148.5
88	103.0
89	103.0
90	82.5
91	62.0
92	38.5
93	15.0
94	8.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	851864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31572724670143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58808602478484	43.92014878015076
2	8.114498829422814	8.328028173486782
3	2.1102581661901083	3.2486829742900776
4	0.9420186623450965	1.9336149095281399
5	0.5474967737345101	1.4047597554704563
6	0.392228122169206	1.2076482801432515
7	0.26784166515396923	0.9621142884040392
8	0.22461700085374356	0.9221107800626244
9	0.17442248792136955	0.8055555134277667
>10	1.3213393570560328	13.74509300243562
>50	0.17466600336551344	6.313850271389128
>100	0.13274564867937963	12.815528847324293
>500	0.007918161499873693	2.716529768196829
>1k	0.0018630968234996921	1.676334655690331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3806	0.4467849328061756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2816	0.3305691988392513	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.1819539269179118	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1251	0.14685442746729527	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1243	0.14591531042513828	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1206	0.14157189410516233	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1107	0.1299503207084699	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1048	0.12302433252256229	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	940	0.11034625245344327	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	888	0.10424199167942301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.869481513481026E-4	0.0	0.0
2	0.0	0.0	5.869481513481026E-4	0.0	0.0
3	0.0	0.0	0.0010565066724265845	1.1738963026962051E-4	0.0
4	0.0	0.0	0.0010565066724265845	1.1738963026962051E-4	0.0
5	1.1738963026962051E-4	0.0	0.001643454823774687	1.1738963026962051E-4	0.0
6	1.1738963026962051E-4	0.0	0.002817351126470892	1.1738963026962051E-4	0.0
7	1.1738963026962051E-4	0.0	0.005869481513481025	1.1738963026962051E-4	0.0
8	2.3477926053924102E-4	0.0	0.00704337781617723	1.1738963026962051E-4	0.0
9	2.3477926053924102E-4	0.0	0.007865105228064573	7.04337781617723E-4	0.0
10	4.6955852107848203E-4	0.0	0.009039001530760779	9.391170421569641E-4	0.0
11	4.6955852107848203E-4	0.0	0.012560690438849394	0.0012912859329658256	0.0
12	4.6955852107848203E-4	0.0	0.01420414526262408	0.0012912859329658256	0.0
13	4.6955852107848203E-4	0.0	0.015260651935050665	0.0012912859329658256	0.0
14	4.6955852107848203E-4	0.0	0.01608237934693801	0.0012912859329658256	0.0
15	5.869481513481026E-4	0.0	0.018430171952330418	0.0012912859329658256	0.0
16	5.869481513481026E-4	0.0	0.02077796455772283	0.001408675563235446	0.0
17	5.869481513481026E-4	0.0	0.02124752307880131	0.001408675563235446	0.0
18	5.869481513481026E-4	0.0	0.021834471230149415	0.001408675563235446	0.0
19	5.869481513481026E-4	0.0	0.022421419381497516	0.001408675563235446	0.0
20	5.869481513481026E-4	0.0	0.022773588272306376	0.001643454823774687	0.0
21	5.869481513481026E-4	0.0	0.0234779260539241	0.0017608444540443075	0.0
22	5.869481513481026E-4	0.0	0.02371270531446334	0.0018782340843139281	0.0
23	5.869481513481026E-4	0.0	0.023947484575002582	0.002817351126470892	1.1738963026962051E-4
24	5.869481513481026E-4	0.0	0.023947484575002582	0.0030521303870101333	1.1738963026962051E-4
25	5.869481513481026E-4	0.0	0.023947484575002582	0.0031695200172797535	1.1738963026962051E-4
26	5.869481513481026E-4	0.0	0.024064874205272202	0.0034042992778189948	1.1738963026962051E-4
27	5.869481513481026E-4	0.0	0.024064874205272202	0.004343416319975958	1.1738963026962051E-4
28	5.869481513481026E-4	1.1738963026962051E-4	0.024064874205272202	0.014438924523163322	1.1738963026962051E-4
29	7.04337781617723E-4	1.1738963026962051E-4	0.024182263835541822	0.0326343172149545	1.1738963026962051E-4
30	7.04337781617723E-4	1.1738963026962051E-4	0.024182263835541822	0.05869481513481025	1.1738963026962051E-4
31	7.04337781617723E-4	1.1738963026962051E-4	0.024182263835541822	0.12032437102636101	1.1738963026962051E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	26.5	1
CTGTCGA	40	0.0019307786	23.125002	9
GTATCAA	2160	0.0	22.61111	1
GTCTATA	55	5.1415915E-4	20.181818	1
CGTAGCG	65	6.899449E-5	19.923077	15
CATAATA	215	0.0	19.790697	2
GACCGTT	60	9.2341425E-4	18.5	7
AAGGTCG	70	1.21872756E-4	18.5	20
CTTAGTG	60	9.2341425E-4	18.5	3
TTTACCG	50	0.0070334254	18.499998	30
TCGTATG	50	0.0070334254	18.499998	37
TTAACGG	270	0.0	17.814814	35
AACGGCC	275	0.0	17.49091	37
GGTCGGC	75	2.066475E-4	17.266666	22
CGTCCTA	75	2.066475E-4	17.266666	33
GTCGGCT	75	2.066475E-4	17.266666	23
TAACGGC	280	0.0	17.178572	36
GCAACGC	65	0.00157948	17.076923	3
GCGGAAT	65	0.00157948	17.076923	19
CCGTTTA	65	0.00157948	17.076923	27
>>END_MODULE