FastQCFastQC Report
Fri 10 Feb 2017
ERR1633249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842790
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55000.652594359211666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.4636979555998529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20780.24656201426215307No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA14720.17465798122901316No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG14430.17121702915316983No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG12640.14997804909882653No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12090.14345210550670984No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11760.13953653935143986No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11190.13277328871960986No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10680.12672195920691987No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10440.12387427473035988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.12328100713107654No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9940.11794159873752653No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9710.11521256778082323No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC9420.11177161570497988No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8930.10595759323200322No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT8870.10524567211286323No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8780.10417779043415322No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC8730.1035845228348699No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT8600.10204202707673324No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA8470.10049953131859657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG358.867424E-426.4285710
GGTATCA10500.025.371431
CGACCAA400.001930761823.12500228
TCGGCGT400.001930761823.12500211
ATCTAAC502.701274E-422.19999920
TACTCGC502.701274E-422.19999920
CGCCGTC603.7247682E-521.58333226
GTATCAA25250.021.4673271
CGGCGTA450.003824973220.55555712
GACCGTT450.003824973220.5555577
CGTAAAT450.003824973220.55555715
GTATTAG3900.019.9230771
TCCGCCG609.234029E-418.531
TGCGGTA609.234029E-418.536
ACGTGAT902.1508295E-618.537
ACTCGCA609.234029E-418.521
ACCCGAG500.00703336418.4999987
TAGTCTG500.00703336418.4999985
TCGTTTG1054.79351E-717.61904712
TACGTGA953.6040783E-617.52631636
Produced by FastQC (version 0.11.5)