##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633249.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842790
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872344237591808	33.0	33.0	33.0	33.0	33.0
2	31.992661279796867	33.0	33.0	33.0	33.0	33.0
3	31.72023042513556	33.0	33.0	33.0	27.0	33.0
4	31.992625683740908	33.0	33.0	33.0	33.0	33.0
5	32.086772505606376	33.0	33.0	33.0	33.0	33.0
6	35.26902431210622	37.0	37.0	37.0	33.0	37.0
7	35.4126698228503	37.0	37.0	37.0	33.0	37.0
8	35.53507160739923	37.0	37.0	37.0	33.0	37.0
9	35.6496707364824	37.0	37.0	37.0	33.0	37.0
10	35.658149717011355	37.0	37.0	37.0	33.0	37.0
11	35.681592092929435	37.0	37.0	37.0	33.0	37.0
12	35.64883541570261	37.0	37.0	37.0	33.0	37.0
13	35.66613153929211	37.0	37.0	37.0	33.0	37.0
14	35.6530666002207	37.0	37.0	37.0	33.0	37.0
15	35.662982474875115	37.0	37.0	37.0	33.0	37.0
16	35.64455795631177	37.0	37.0	37.0	33.0	37.0
17	35.64123328468539	37.0	37.0	37.0	33.0	37.0
18	35.63626407527379	37.0	37.0	37.0	33.0	37.0
19	35.61444250643695	37.0	37.0	37.0	33.0	37.0
20	35.61363328943153	37.0	37.0	37.0	33.0	37.0
21	35.60826184458762	37.0	37.0	37.0	33.0	37.0
22	35.52270435102457	37.0	37.0	37.0	33.0	37.0
23	35.56660615337154	37.0	37.0	37.0	33.0	37.0
24	35.556040057428305	37.0	37.0	37.0	33.0	37.0
25	35.58693506092858	37.0	37.0	37.0	33.0	37.0
26	35.4784750649628	37.0	37.0	37.0	33.0	37.0
27	35.48479336489517	37.0	37.0	37.0	33.0	37.0
28	35.5042181326309	37.0	37.0	37.0	33.0	37.0
29	35.51206706296942	37.0	37.0	37.0	33.0	37.0
30	35.51099087554432	37.0	37.0	37.0	33.0	37.0
31	35.49682483180864	37.0	37.0	37.0	33.0	37.0
32	35.4846853901921	37.0	37.0	37.0	33.0	37.0
33	35.48484319937351	37.0	37.0	37.0	33.0	37.0
34	35.46657411692118	37.0	37.0	37.0	33.0	37.0
35	35.38107713665326	37.0	37.0	37.0	33.0	37.0
36	35.41381008317612	37.0	37.0	37.0	33.0	37.0
37	35.39679042228788	37.0	37.0	37.0	33.0	37.0
38	35.3958578056218	37.0	37.0	37.0	33.0	37.0
39	35.29149135609108	37.0	37.0	37.0	33.0	37.0
40	35.07386181611078	37.0	37.0	37.0	33.0	37.0
41	35.23833457919529	37.0	37.0	37.0	33.0	37.0
42	35.26602831072984	37.0	37.0	37.0	33.0	37.0
43	34.941948765410125	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	16.0
16	36.0
17	29.0
18	27.0
19	22.0
20	62.0
21	135.0
22	401.0
23	848.0
24	1844.0
25	3174.0
26	5379.0
27	7960.0
28	11331.0
29	15550.0
30	20522.0
31	26426.0
32	33158.0
33	44281.0
34	64299.0
35	130669.0
36	476619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15462926707721	20.889664091885287	13.523772232703282	23.431934408334225
2	16.12584392315998	22.10965958305153	39.23456614340464	22.529930350383847
3	17.67747600232561	27.69266365286726	29.95538627653389	24.674474068273238
4	11.805669265178752	18.097272155578494	40.511515324102085	29.58554325514066
5	13.63637442304726	37.06178288778937	35.577427354382465	13.724415334780907
6	28.75235823870715	40.86130590063954	17.303717414777108	13.082618445876196
7	26.93197593706617	32.20766739045314	23.47168333748621	17.388673334994483
8	24.36253396457006	35.73072770203728	20.680121975818412	19.226616357574247
9	25.775341425503388	15.26097841692474	20.720226865529966	38.243453292041906
10	15.082642176580169	28.128240724261083	33.96634986176865	22.822767237390096
11	33.66698703117028	24.361703389931062	23.789318810142504	18.181990768756155
12	22.978322001922187	26.231089595272845	30.276581354785893	20.514007048019078
13	27.50163148589803	22.902146442174207	26.5507421777667	23.045479894161062
14	21.9506638664436	21.514730834490205	28.30444120124824	28.230164097817962
15	23.526619917179843	29.086130590063952	25.746627273698074	21.640622219058127
16	22.951980920514007	28.83743281244438	25.32315286132963	22.88743340571198
17	22.446754232964324	27.478968663605407	26.342386596898397	23.731890506531876
18	22.3564589043534	26.92390749771592	28.967833030766858	21.751800567163826
19	25.087151010334725	26.373236512061133	28.085288150072973	20.454324327531175
20	24.206148625398974	26.514315547170707	28.311560412439636	20.967975414990686
21	22.44212674568991	27.384639115319352	28.216518943034448	21.95671519595629
22	22.7586943366675	26.673311263778636	28.273709939605357	22.294284459948503
23	21.904033033139928	26.9475195481674	29.034516308926307	22.113931109766373
24	22.262603970146774	28.075795868484438	27.225880705751138	22.43571945561765
25	22.293097924749937	27.693968841585686	28.600363079770762	21.412570153893615
26	23.199373509415157	27.09287010999181	27.99428090034291	21.71347548025012
27	22.65570308143191	27.076851884811166	27.304666642935967	22.962778390820965
28	22.03633170778011	26.85758018011604	28.920252969304332	22.18583514279951
29	23.724296681261052	27.267172130661255	27.72683586658598	21.28169532149171
30	23.222629599307062	26.583253242207427	28.01243488888098	22.181682269604526
31	22.39549591238624	26.53448664554634	28.417873966231213	22.652143475836212
32	21.456827916800155	26.909194461253694	28.49143914854234	23.142538473403814
33	21.723323722398224	26.6227648643197	29.02644786957605	22.627463543706025
34	22.821818009231244	26.654564007641284	28.76161321325597	21.7620047698715
35	22.688332799392494	27.514327412522693	27.489291519832936	22.30804826825188
36	22.978084694882472	27.033187389503908	27.35699284519275	22.631735070420863
37	22.631735070420863	26.636291365583357	28.17926173779945	22.552711826196322
38	22.47250204677322	26.69443159031313	27.681035607921313	23.152030754992346
39	22.09091232691418	26.75838583751587	28.39010904258475	22.760592792985204
40	22.615716845240215	27.69195173174812	28.04233557588486	21.649995847126803
41	22.459331506069127	26.580998825330155	28.108306932925164	22.851362735675554
42	21.601941171584855	28.46094519393918	27.645914166043735	22.291199468432232
43	23.18038894623809	26.126437190759265	27.54185502912944	23.15131883387321
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	80.5
2	97.0
3	366.0
4	635.0
5	635.0
6	763.5
7	892.0
8	847.0
9	802.0
10	1055.0
11	1308.0
12	1308.0
13	2075.0
14	2842.0
15	5035.0
16	7228.0
17	8208.0
18	9188.0
19	9188.0
20	8394.0
21	7600.0
22	8314.0
23	9028.0
24	11145.5
25	13263.0
26	13263.0
27	16227.0
28	19191.0
29	22072.5
30	24954.0
31	28958.5
32	32963.0
33	32963.0
34	41503.0
35	50043.0
36	53345.5
37	56648.0
38	58151.0
39	59654.0
40	59654.0
41	60586.0
42	61518.0
43	62970.0
44	64422.0
45	62608.0
46	60794.0
47	60794.0
48	57929.5
49	55065.0
50	56694.0
51	58323.0
52	58479.0
53	58635.0
54	58635.0
55	56411.0
56	54187.0
57	45153.0
58	36119.0
59	33227.0
60	30335.0
61	30335.0
62	27020.0
63	23705.0
64	18334.0
65	12963.0
66	11079.0
67	9195.0
68	9195.0
69	7908.0
70	6621.0
71	6583.0
72	6545.0
73	5812.0
74	5079.0
75	5079.0
76	3157.5
77	1236.0
78	913.0
79	590.0
80	489.5
81	389.0
82	389.0
83	319.0
84	249.0
85	231.5
86	214.0
87	170.0
88	126.0
89	126.0
90	94.0
91	62.0
92	39.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	842790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.913948544444935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11615889128683	45.22543790071953
2	7.52268334177076	7.810643918416805
3	1.8042187118002002	2.809923521019609
4	0.8052011746754663	1.6720468940011508
5	0.4787510546435742	1.2426928808182627
6	0.3303339792036148	1.028936471931492
7	0.23150433986823676	0.8412813071414735
8	0.18553956681342482	0.770567321960859
9	0.139027891712757	0.6495738134956857
>10	1.0753370655548367	11.302357857766898
>50	0.15457778223354232	5.606713131699957
>100	0.14343661017793116	14.638605501055594
>500	0.01044441336227675	3.711854220156569
>1k	0.002553078821889872	2.0266632468685803
>5k	2.320980747172611E-4	0.6627020129475144
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5500	0.652594359211666	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3908	0.4636979555998529	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2078	0.24656201426215307	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1472	0.17465798122901316	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1443	0.17121702915316983	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1264	0.14997804909882653	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1209	0.14345210550670984	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1176	0.13953653935143986	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1119	0.13277328871960986	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1068	0.12672195920691987	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1044	0.12387427473035988	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.12328100713107654	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	994	0.11794159873752653	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	971	0.11521256778082323	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	942	0.11177161570497988	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	893	0.10595759323200322	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	887	0.10524567211286323	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	878	0.10417779043415322	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	873	0.1035845228348699	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	860	0.10204202707673324	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	847	0.10049953131859657	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.1865351985666655E-4	0.0	0.0
2	1.1865351985666655E-4	0.0	2.373070397133331E-4	0.0	0.0
3	1.1865351985666655E-4	0.0	3.5596055956999966E-4	0.0	0.0
4	1.1865351985666655E-4	0.0	3.5596055956999966E-4	0.0	0.0
5	1.1865351985666655E-4	0.0	5.932675992833328E-4	0.0	0.0
6	1.1865351985666655E-4	0.0	0.0011865351985666656	0.0	0.0
7	1.1865351985666655E-4	0.0	0.0037969126354133295	1.1865351985666655E-4	0.0
8	1.1865351985666655E-4	0.0	0.004271526714839996	2.373070397133331E-4	0.0
9	1.1865351985666655E-4	0.0	0.004746140794266662	8.305746389966658E-4	0.0
10	2.373070397133331E-4	0.0	0.005220754873693328	0.001067881678709999	0.0
11	2.373070397133331E-4	0.0	0.008780360469393324	0.001067881678709999	0.0
12	2.373070397133331E-4	0.0	0.009492281588533324	0.001305188718423332	0.0
13	2.373070397133331E-4	0.0	0.01067881678709999	0.001305188718423332	0.0
14	2.373070397133331E-4	0.0	0.010797470306956656	0.001305188718423332	0.0
15	2.373070397133331E-4	0.0	0.01233996606509332	0.001305188718423332	0.0
16	2.373070397133331E-4	0.0	0.01340784774380332	0.001542495758136665	0.0
17	2.373070397133331E-4	0.0	0.014001115343086653	0.001542495758136665	0.0
18	2.373070397133331E-4	0.0	0.014238422382799985	0.0016611492779933317	0.0
19	2.373070397133331E-4	0.0	0.014357075902656653	0.0016611492779933317	0.0
20	2.373070397133331E-4	0.0	0.014831689982083318	0.002017109837563331	0.0
21	2.373070397133331E-4	0.0	0.014950343501939986	0.0022544168772766645	0.0
22	2.373070397133331E-4	0.0	0.01542495758136665	0.002610377436846664	0.0
23	2.373070397133331E-4	0.0	0.015543611101223319	0.00308499151627333	0.0
24	2.373070397133331E-4	0.0	0.015543611101223319	0.003678259115556663	0.0
25	2.373070397133331E-4	0.0	0.015662264621079985	0.003915566155269996	0.0
26	2.373070397133331E-4	0.0	0.015899571660793317	0.004508833754553329	0.0
27	2.373070397133331E-4	0.0	0.015899571660793317	0.00616998303254666	0.0
28	2.373070397133331E-4	0.0	0.015899571660793317	0.016374185740219982	0.0
29	3.5596055956999966E-4	0.0	0.015899571660793317	0.03690124467542329	0.0
30	3.5596055956999966E-4	0.0	0.015899571660793317	0.0645475148020266	0.0
31	3.5596055956999966E-4	0.0	0.015899571660793317	0.1251794634487832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGCG	35	8.867424E-4	26.42857	10
GGTATCA	1050	0.0	25.37143	1
CGACCAA	40	0.0019307618	23.125002	28
TCGGCGT	40	0.0019307618	23.125002	11
ATCTAAC	50	2.701274E-4	22.199999	20
TACTCGC	50	2.701274E-4	22.199999	20
CGCCGTC	60	3.7247682E-5	21.583332	26
GTATCAA	2525	0.0	21.467327	1
CGGCGTA	45	0.0038249732	20.555557	12
GACCGTT	45	0.0038249732	20.555557	7
CGTAAAT	45	0.0038249732	20.555557	15
GTATTAG	390	0.0	19.923077	1
TCCGCCG	60	9.234029E-4	18.5	31
TGCGGTA	60	9.234029E-4	18.5	36
ACGTGAT	90	2.1508295E-6	18.5	37
ACTCGCA	60	9.234029E-4	18.5	21
ACCCGAG	50	0.007033364	18.499998	7
TAGTCTG	50	0.007033364	18.499998	5
TCGTTTG	105	4.79351E-7	17.619047	12
TACGTGA	95	3.6040783E-6	17.526316	36
>>END_MODULE