FastQCFastQC Report
Fri 10 Feb 2017
ERR1633248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670402
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47910.7146458393620544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.5184948732253185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21140.3153331881468134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.13036953947034763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG200.001841388537.032
ATCTCGT358.8655227E-426.42857237
GGTATCA8600.025.383721
CGGCCTT751.371518E-824.66666624
GTATCAA22000.022.2840921
GGGCGTA502.7004755E-422.19999937
TCGCCAT705.09669E-621.14285913
ATAGAAC450.00382417120.5555553
TATTGCG450.00382417120.5555559
CGCTCTC1351.8189894E-1220.55555529
TCTATCG450.00382417120.55555529
GAGTCGT555.1400217E-420.18181819
GTCGCCA656.896647E-519.92307712
TTCGCCG1303.274181E-1119.92307724
GCCGGCA1403.6379788E-1219.8214315
GCCGCTC1457.2759576E-1219.1379327
TAGAACA1501.2732926E-1118.54
CCGCTCT1501.2732926E-1118.528
TTAAGGT609.2313427E-418.54
CAAGCGT701.2182355E-418.516
Produced by FastQC (version 0.11.5)