FastQCFastQC Report
Fri 10 Feb 2017
ERR1633247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687365
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157372.289467750030915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117211.7052075680315408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63170.919016825122024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.40051501022018865No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG23700.3447949779229376No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21880.3183170513482647No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18470.2687073097990151No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17510.2547409309464404No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16830.24484807925919996No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.2429567987895805No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14490.21080503080604918No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13980.20338539204061887No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13590.19771155063176044No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13320.1937835065794738No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12850.18694580026623409No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12650.18403613800528104No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10380.15101147134346382No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10130.1473743935172725No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.13849992362136565No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9430.13719057560393677No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9390.13660864315174617No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9280.13500832890822198No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9150.13311704843860248No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9090.13224414976031656No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA9050.13166221730812597No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8970.13049835240374474No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8820.12831610570802993No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8750.1272977239166964No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.12496999410793391No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8470.12322419675136209No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8420.12249678118612381No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8340.1213329162817426No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8320.12104195005564729No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.12016905137736138No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8050.11711390600336066No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7920.11522262553374117No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7750.11274941261193107No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7460.10853040233354913No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7440.10823943610745383No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7430.10809395299440616No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7400.10765750365526322No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7390.10751202054221556No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7230.10518429073345312No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7060.10271107781164301No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG7010.10198366224640475No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7010.10198366224640475No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6890.10023786488983291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.2331092E-437.02
ACGATTC250.005494824629.68
CGCGAGT358.865753E-426.4285718
TTCGCCG5900.026.02542324
GCCGGCA6500.025.61538515
GCCGCTC5950.025.18487527
CCGCTCT6050.025.07437928
TACACAG451.322456E-424.6666685
ACAACGC751.3716999E-824.6666663
CGCTCTC6100.024.56557529
CCGGCAG6800.024.21323616
CGCCGGC7050.023.87943514
GCTTCGC6850.023.76642422
CGGCAGC6950.023.69064717
AACAACG551.9012161E-523.5454542
GTTATGC551.9012161E-523.5454541
GGTATCA25000.023.4580021
AGCTTCG6950.023.4244621
CATTACC400.001930401123.1250022
CTACGCT400.001930401123.12500228
Produced by FastQC (version 0.11.5)