##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687365
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91668182115761	33.0	33.0	33.0	33.0	33.0
2	32.01192234111426	33.0	33.0	33.0	33.0	33.0
3	31.73687051275523	33.0	33.0	33.0	27.0	33.0
4	32.007371629338124	33.0	33.0	33.0	33.0	33.0
5	32.103575247503144	33.0	33.0	33.0	33.0	33.0
6	35.23127159514959	37.0	37.0	37.0	33.0	37.0
7	35.399313319706415	37.0	37.0	37.0	33.0	37.0
8	35.54024717580907	37.0	37.0	37.0	33.0	37.0
9	35.65810595535123	37.0	37.0	37.0	33.0	37.0
10	35.63074349144923	37.0	37.0	37.0	33.0	37.0
11	35.66003651626138	37.0	37.0	37.0	33.0	37.0
12	35.647805750947455	37.0	37.0	37.0	33.0	37.0
13	35.65568366151899	37.0	37.0	37.0	33.0	37.0
14	35.64076727793822	37.0	37.0	37.0	33.0	37.0
15	35.6816000232773	37.0	37.0	37.0	33.0	37.0
16	35.65719232140129	37.0	37.0	37.0	33.0	37.0
17	35.66302764906563	37.0	37.0	37.0	33.0	37.0
18	35.63040451579583	37.0	37.0	37.0	33.0	37.0
19	35.60898940155521	37.0	37.0	37.0	33.0	37.0
20	35.6096237079281	37.0	37.0	37.0	33.0	37.0
21	35.59045630778407	37.0	37.0	37.0	33.0	37.0
22	35.4970139591047	37.0	37.0	37.0	33.0	37.0
23	35.53390265724906	37.0	37.0	37.0	33.0	37.0
24	35.54916965513228	37.0	37.0	37.0	33.0	37.0
25	35.554311028347385	37.0	37.0	37.0	33.0	37.0
26	35.44356346337099	37.0	37.0	37.0	33.0	37.0
27	35.45561091996247	37.0	37.0	37.0	33.0	37.0
28	35.46592858233981	37.0	37.0	37.0	33.0	37.0
29	35.460701374088	37.0	37.0	37.0	33.0	37.0
30	35.445259796469124	37.0	37.0	37.0	33.0	37.0
31	35.421275450452086	37.0	37.0	37.0	33.0	37.0
32	35.39117062986914	37.0	37.0	37.0	33.0	37.0
33	35.3474951445011	37.0	37.0	37.0	33.0	37.0
34	35.27997643173568	37.0	37.0	37.0	33.0	37.0
35	35.1882711514261	37.0	37.0	37.0	33.0	37.0
36	35.167327402471756	37.0	37.0	37.0	33.0	37.0
37	35.1306060099074	37.0	37.0	37.0	33.0	37.0
38	35.05889447382395	37.0	37.0	37.0	33.0	37.0
39	34.915125151848	37.0	37.0	37.0	27.0	37.0
40	34.65036334407483	37.0	37.0	37.0	27.0	37.0
41	34.74938206047733	37.0	37.0	37.0	27.0	37.0
42	34.686357321073956	37.0	37.0	37.0	27.0	37.0
43	34.28584522051603	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	22.0
16	49.0
17	42.0
18	36.0
19	46.0
20	56.0
21	144.0
22	390.0
23	796.0
24	1569.0
25	2893.0
26	4608.0
27	6859.0
28	10241.0
29	13680.0
30	18524.0
31	23488.0
32	29858.0
33	38422.0
34	54041.0
35	105240.0
36	376359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42782946469489	17.956107744793524	14.982141947873401	21.63392084263819
2	16.15371745724615	23.107228328471773	39.1039695067395	21.63508470754257
3	18.978708546405475	28.85293839517578	30.49107824809235	21.67727481032639
4	11.742087537189121	17.9978613982382	39.66975333338183	30.59029773119085
5	14.788940373746119	35.71275814159872	31.875204585627724	17.623096899027445
6	26.595185963789252	41.12342059895398	16.24289860554436	16.03849483171241
7	25.268670939020755	34.11593549278768	21.015035679733472	19.6003578884581
8	25.998268750954733	31.988681413804894	19.69303063146945	22.320019203770922
9	29.044103205720397	13.250456453267187	18.855338866541064	38.85010147447135
10	17.01410458780997	26.69062288594851	30.10205640380293	26.19321612243859
11	34.52590690535596	24.395917743847882	21.455994995380912	19.622180355415246
12	25.078233544041375	26.568417070988488	27.78189171691896	20.57145766805118
13	31.606206309602612	22.65812195849367	24.85855404333942	20.8771176885643
14	24.194860081616028	21.29800033461116	29.819673681377434	24.68746590239538
15	26.296800098928518	26.037403708364554	26.96223985800848	20.70355633469845
16	20.286747215816924	28.065438304248836	26.286616281015178	25.36119819891906
17	22.328020774988545	24.881249408974853	25.90865115331738	26.882078662719227
18	25.67369592574542	21.681348337491727	29.444618215940583	23.20033752082227
19	27.23749390789464	22.94516014053669	30.49064179875321	19.326704152815463
20	26.78562335876863	21.55521447847941	29.97432223054709	21.68483993220487
21	23.248783397467136	24.636983262167846	30.402915481585474	21.71131785877954
22	23.25372982331076	24.110479876048387	30.466782568213397	22.16900773242746
23	22.352025488641406	24.023190008219796	31.368486902882747	22.25629760025605
24	22.547263826351358	23.94797523877416	31.41009507321438	22.094665861660108
25	22.349406792606548	24.257563303339566	32.19992289395008	21.1931070101038
26	22.711659744095204	24.52859834294734	30.759349108552225	22.000392804405227
27	23.316142078808202	24.014751987663033	31.44239232431096	21.22671360921781
28	21.997337659031228	24.449164563223324	30.38458460934147	23.168913168403975
29	22.92071897754468	24.278076422279284	30.000218224669574	22.80098637550646
30	23.200192037709222	24.128228815840203	30.969717690019134	21.701861456431445
31	22.88856720956115	24.03526510660275	30.602081863347713	22.474085820488387
32	21.177831283233797	24.17361954711107	31.263157129036244	23.385392040618886
33	21.388927280265943	24.45571130331047	31.558924297862124	22.596437118561465
34	21.6611261847781	24.442181373797037	30.785536068900804	23.111156372524057
35	21.42078808202338	25.30475075105657	30.979174092367227	22.29528707455282
36	22.255424701577763	24.683974307682234	31.20219970466928	21.85840128607072
37	22.97221999956355	25.141373215104057	30.31897172535698	21.567435059975413
38	22.59861936525718	25.220661511715026	29.144777519949372	23.03594160307842
39	22.12565376473926	24.35518247219454	29.90972772835393	23.60943603471227
40	22.15533231980098	25.664821455849513	30.197347842849144	21.982498381500367
41	22.899914892378867	24.599157652775457	29.629818218850247	22.871109235995434
42	21.295236155463254	25.807976839088404	29.995562765052046	22.901224240396296
43	22.053348657554576	25.82543481265412	29.24341507059568	22.877801459195624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	103.5
2	185.0
3	760.5
4	1336.0
5	1336.0
6	1700.0
7	2064.0
8	2010.0
9	1956.0
10	2668.0
11	3380.0
12	3380.0
13	5492.5
14	7605.0
15	13577.5
16	19550.0
17	20885.5
18	22221.0
19	22221.0
20	17459.0
21	12697.0
22	9570.5
23	6444.0
24	6625.0
25	6806.0
26	6806.0
27	7508.5
28	8211.0
29	8883.5
30	9556.0
31	10980.5
32	12405.0
33	12405.0
34	15247.5
35	18090.0
36	19134.5
37	20179.0
38	22754.0
39	25329.0
40	25329.0
41	29132.5
42	32936.0
43	36460.5
44	39985.0
45	47452.0
46	54919.0
47	54919.0
48	58728.5
49	62538.0
50	66793.0
51	71048.0
52	69502.5
53	67957.0
54	67957.0
55	60397.0
56	52837.0
57	46343.0
58	39849.0
59	35968.0
60	32087.0
61	32087.0
62	26286.5
63	20486.0
64	15903.0
65	11320.0
66	10392.0
67	9464.0
68	9464.0
69	7725.5
70	5987.0
71	4736.0
72	3485.0
73	3067.0
74	2649.0
75	2649.0
76	1726.0
77	803.0
78	586.5
79	370.0
80	302.5
81	235.0
82	235.0
83	193.0
84	151.0
85	132.5
86	114.0
87	85.0
88	56.0
89	56.0
90	49.0
91	42.0
92	26.5
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	687365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.33809542734097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54659846650681	32.47225967677843
2	9.005819622707147	7.085435834389471
3	2.63312818362541	3.107467432796321
4	1.352571893112778	2.1283040881443878
5	0.7868658323414901	1.547690160058181
6	0.5296977071394722	1.250237937065776
7	0.39643047268550546	1.091637383536581
8	0.3132789621429352	0.98590381665257
9	0.23217458685562725	0.8219975448177116
>10	1.7514116908610686	13.813660209486889
>50	0.24090277729739862	6.6185324513953185
>100	0.18133683227210112	14.158184039087354
>500	0.02299697872889432	6.290927846837614
>1k	0.005654994769400242	3.618777679593687
>5k	3.769996512933495E-4	0.9368394164990501
>10k+	7.53999302586699E-4	4.07214448286068
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15737	2.289467750030915	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11721	1.7052075680315408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6317	0.919016825122024	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2753	0.40051501022018865	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	2370	0.3447949779229376	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2188	0.3183170513482647	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1847	0.2687073097990151	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1751	0.2547409309464404	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1683	0.24484807925919996	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1670	0.2429567987895805	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1449	0.21080503080604918	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	1398	0.20338539204061887	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1359	0.19771155063176044	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1332	0.1937835065794738	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1285	0.18694580026623409	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1265	0.18403613800528104	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1038	0.15101147134346382	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1013	0.1473743935172725	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	952	0.13849992362136565	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	943	0.13719057560393677	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	939	0.13660864315174617	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	928	0.13500832890822198	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	915	0.13311704843860248	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	909	0.13224414976031656	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	905	0.13166221730812597	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	897	0.13049835240374474	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	882	0.12831610570802993	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	875	0.1272977239166964	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	859	0.12496999410793391	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	847	0.12322419675136209	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	842	0.12249678118612381	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	834	0.1213329162817426	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	832	0.12104195005564729	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.12016905137736138	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	805	0.11711390600336066	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	792	0.11522262553374117	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	775	0.11274941261193107	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	746	0.10853040233354913	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	744	0.10823943610745383	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	743	0.10809395299440616	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	740	0.10765750365526322	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	739	0.10751202054221556	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	723	0.10518429073345312	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	706	0.10271107781164301	No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG	701	0.10198366224640475	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	701	0.10198366224640475	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	689	0.10023786488983291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.27415565238265E-4	0.0	0.0
2	4.36449339142959E-4	0.0	8.72898678285918E-4	0.0	0.0
3	4.36449339142959E-4	0.0	0.00145483113047653	0.0	0.0
4	4.36449339142959E-4	0.0	0.002618696034857754	0.0	0.0
5	5.81932452190612E-4	0.0	0.00436449339142959	0.0	0.0
6	7.27415565238265E-4	0.0	0.009019953008954485	0.0	0.0
7	7.27415565238265E-4	0.0	0.02182246695714795	0.0	0.0
8	8.72898678285918E-4	0.0	0.02589599412248223	0.0	0.0
9	0.001018381791333571	0.0	0.03127886930524539	8.72898678285918E-4	0.0
10	0.003928044052286631	0.0	0.03477046401838906	8.72898678285918E-4	0.0
11	0.003928044052286631	0.0	0.049464258436202016	0.001018381791333571	0.0
12	0.003928044052286631	0.0	0.05659293097553701	0.001163864904381224	0.0
13	0.003928044052286631	0.0	0.06081194125391895	0.00145483113047653	0.0
14	0.003928044052286631	0.0	0.06343063728877671	0.00145483113047653	0.0
15	0.004073527165334284	0.0	0.07026834360201639	0.002036763582667142	0.0
16	0.004219010278381937	0.0	0.07506928633258894	0.002618696034857754	0.0
17	0.004509976504477243	0.0	0.07666960057611313	0.0032006284870483657	0.0
18	0.004509976504477243	0.0	0.07885184727182792	0.003928044052286631	0.0
19	0.004655459617524896	0.0	0.0804521615153521	0.004219010278381937	0.0
20	0.004655459617524896	0.0	0.08277989132411455	0.005091908956667855	0.0
21	0.0048009427305725485	0.0	0.08583503669811526	0.00581932452190612	0.0
22	0.004946425843620201	0.0	0.08787180028078241	0.007710604991525608	0.0
23	0.004946425843620201	0.0	0.08889018207211598	0.010038334800288056	0.0
24	0.005091908956667855	0.0	0.08889018207211598	0.012366064609050504	0.0
25	0.005382875182763161	0.0	0.08918114829821129	0.012947997061241116	0.0
26	0.005528358295810814	0.0	0.08918114829821129	0.014402828191717646	0.0
27	0.005528358295810814	0.0	0.08947211452430659	0.01905828780924254	0.0
28	0.005528358295810814	0.0	0.08961759763735425	0.04320848457515294	0.0
29	0.005528358295810814	0.0	0.08961759763735425	0.10605718941173903	0.0
30	0.005673841408858467	0.0	0.08961759763735425	0.19291060790118786	0.0
31	0.005673841408858467	0.0	0.08961759763735425	0.32704603813112393	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAACG	25	1.2331092E-4	37.0	2
ACGATTC	25	0.0054948246	29.6	8
CGCGAGT	35	8.865753E-4	26.42857	18
TTCGCCG	590	0.0	26.025423	24
GCCGGCA	650	0.0	25.615385	15
GCCGCTC	595	0.0	25.184875	27
CCGCTCT	605	0.0	25.074379	28
TACACAG	45	1.322456E-4	24.666668	5
ACAACGC	75	1.3716999E-8	24.666666	3
CGCTCTC	610	0.0	24.565575	29
CCGGCAG	680	0.0	24.213236	16
CGCCGGC	705	0.0	23.879435	14
GCTTCGC	685	0.0	23.766424	22
CGGCAGC	695	0.0	23.690647	17
AACAACG	55	1.9012161E-5	23.545454	2
GTTATGC	55	1.9012161E-5	23.545454	1
GGTATCA	2500	0.0	23.458002	1
AGCTTCG	695	0.0	23.42446	21
CATTACC	40	0.0019304011	23.125002	2
CTACGCT	40	0.0019304011	23.125002	28
>>END_MODULE