FastQCFastQC Report
Fri 10 Feb 2017
ERR1633246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360448
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11380.31571821732954547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.2344304865056818No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG6170.1711758700284091No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG5410.15009099786931818No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT5250.14565207741477273No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT5120.14204545454545456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4890.13566450639204544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT250.00549140129.5999986
GACGTAT951.0913936E-1125.3157926
GGTATCA1950.024.6666681
GTATCAA5700.024.0175441
GGGTATA400.001928629523.1250021
AGTACCG753.726036E-722.25
ACGTATG1107.4578566E-1121.86363627
CAATCCA1107.4578566E-1121.86363619
ACGAGGG603.7171565E-521.58333415
TATATAC705.0859035E-621.1428573
TAGTACC450.003820790220.5555554
CAGACGT1202.3646862E-1020.04166824
GTGTGGG1850.020.01
CCCAATC1254.0927262E-1019.2417
GGCCGTA801.6116579E-518.514
TATGAGT1306.91216E-1018.530
GTGTATA701.2162529E-418.51
GATTGTA801.6116579E-518.523
CTCTAAT609.2200533E-418.51
GGTCATG1700.018.4999987
Produced by FastQC (version 0.11.5)