##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360448
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89781327681108	33.0	33.0	33.0	33.0	33.0
2	31.93657060102983	33.0	33.0	33.0	33.0	33.0
3	31.916681463068183	33.0	33.0	33.0	33.0	33.0
4	32.01897083629262	33.0	33.0	33.0	33.0	33.0
5	32.03857976740057	33.0	33.0	33.0	33.0	33.0
6	35.26372736150568	37.0	37.0	37.0	33.0	37.0
7	35.42957930131392	37.0	37.0	37.0	33.0	37.0
8	35.53956465287642	37.0	37.0	37.0	33.0	37.0
9	35.66531649502841	37.0	37.0	37.0	33.0	37.0
10	35.66314697265625	37.0	37.0	37.0	33.0	37.0
11	35.68097201260653	37.0	37.0	37.0	33.0	37.0
12	35.668701171875	37.0	37.0	37.0	33.0	37.0
13	35.65787852894176	37.0	37.0	37.0	33.0	37.0
14	35.66576316139915	37.0	37.0	37.0	33.0	37.0
15	35.650842840021305	37.0	37.0	37.0	33.0	37.0
16	35.61815851384943	37.0	37.0	37.0	33.0	37.0
17	35.59849687056108	37.0	37.0	37.0	33.0	37.0
18	35.59463778409091	37.0	37.0	37.0	33.0	37.0
19	35.58746615323153	37.0	37.0	37.0	33.0	37.0
20	35.603235418146305	37.0	37.0	37.0	33.0	37.0
21	35.58185369318182	37.0	37.0	37.0	33.0	37.0
22	35.570825750177555	37.0	37.0	37.0	33.0	37.0
23	35.5704872824929	37.0	37.0	37.0	33.0	37.0
24	35.57408558238637	37.0	37.0	37.0	33.0	37.0
25	35.57623291015625	37.0	37.0	37.0	33.0	37.0
26	35.52974354137074	37.0	37.0	37.0	33.0	37.0
27	35.493566339666195	37.0	37.0	37.0	33.0	37.0
28	35.48745450106534	37.0	37.0	37.0	33.0	37.0
29	35.483551025390625	37.0	37.0	37.0	33.0	37.0
30	35.48806485262784	37.0	37.0	37.0	33.0	37.0
31	35.46816739169034	37.0	37.0	37.0	33.0	37.0
32	35.46124822443182	37.0	37.0	37.0	33.0	37.0
33	35.45037286931818	37.0	37.0	37.0	33.0	37.0
34	35.43393221768466	37.0	37.0	37.0	33.0	37.0
35	35.422149658203125	37.0	37.0	37.0	33.0	37.0
36	35.42058216441762	37.0	37.0	37.0	33.0	37.0
37	35.40292080965909	37.0	37.0	37.0	33.0	37.0
38	35.366185968572445	37.0	37.0	37.0	33.0	37.0
39	35.38235196200284	37.0	37.0	37.0	33.0	37.0
40	35.36510398171165	37.0	37.0	37.0	33.0	37.0
41	35.35514415394176	37.0	37.0	37.0	33.0	37.0
42	35.35540771484375	37.0	37.0	37.0	33.0	37.0
43	35.12605424360795	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	12.0
17	23.0
18	10.0
19	11.0
20	17.0
21	62.0
22	170.0
23	388.0
24	805.0
25	1454.0
26	2371.0
27	3457.0
28	5058.0
29	6834.0
30	8658.0
31	11144.0
32	14152.0
33	18672.0
34	26049.0
35	48846.0
36	212242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81407581676137	20.206798206676137	13.169167258522727	24.809958718039773
2	16.06722745028409	21.137306906960227	38.41719193892045	24.378273703835227
3	18.378795276988637	25.794566761363637	29.847856001420453	25.97878196022727
4	13.074840198863635	17.194713245738637	38.69712136008523	31.0333251953125
5	14.394309303977273	36.71014959161932	34.87909490411932	14.016446200284092
6	30.82497336647727	38.95652077414773	16.77273837002841	13.445767489346592
7	27.439741654829547	31.44808682528409	22.813554243607957	18.29861727627841
8	25.319324840198863	35.47307794744318	20.311112837357957	18.896484375
9	25.737415660511363	15.227716619318182	20.121626420454543	38.913241299715914
10	14.818503639914773	28.029285777698863	33.57627175071023	23.575938831676137
11	34.50483842329545	23.170609907670457	23.944091796875	18.38045987215909
12	23.328191583806817	25.0518798828125	30.27066317471591	21.349265358664773
13	28.364424272017047	22.21957120028409	25.864757191051137	23.551247336647727
14	22.486738725142043	20.379083806818183	26.91678133877841	30.217396129261363
15	23.879450017755683	28.99447354403409	24.031760475852273	23.094315962357957
16	23.73046875	27.900279651988637	24.55472079190341	23.814530806107957
17	22.667347301136363	27.193104137073863	26.031494140625	24.108054421164773
18	23.780129172585227	26.976151899857953	27.019153941761363	22.224564985795457
19	24.98779296875	26.215154474431817	26.70565518465909	22.09139737215909
20	24.177689985795457	26.812744140625	26.738114790482953	22.27145108309659
21	23.231090198863637	26.669866388494317	27.28493430397727	22.814109108664773
22	23.226373845880683	26.262872869318183	27.28909579190341	23.221657492897727
23	23.186146129261363	26.507568359375	27.069646661931817	23.236638849431817
24	23.161732066761363	26.96560946377841	26.080322265625	23.792336203835227
25	23.511574485085227	27.18783291903409	26.551957563920453	22.748635031960227
26	23.780406605113637	26.34249600497159	26.88987038352273	22.987227006392043
27	23.399491743607957	26.66404030539773	26.74893465909091	23.18753329190341
28	23.216663707386363	26.03066184303977	27.295476740056817	23.457197709517043
29	23.776799982244317	26.246226917613637	26.58108797940341	23.395885120738637
30	23.561512340198863	26.42544833096591	27.0111083984375	23.001930930397727
31	23.355934836647727	26.600785688920453	27.106822620738637	22.936456853693183
32	23.157570578835227	26.42794522372159	27.12790749289773	23.286576704545457
33	23.418079723011363	26.291448419744317	27.3162841796875	22.974187677556817
34	23.615334250710227	26.09336159446023	26.97531960227273	23.315984552556817
35	23.283247514204543	26.844648881392047	26.811079545454547	23.061024058948863
36	23.512684215198863	26.215154474431817	27.1484375	23.123723810369317
37	23.63863858309659	26.179920543323863	26.776123046875	23.405317826704543
38	23.832286487926137	26.423506303267047	26.871282404119317	22.8729248046875
39	23.304054953835227	26.23623934659091	26.9073486328125	23.552357066761363
40	23.105413263494317	27.147050337357953	26.925104314630683	22.822432084517043
41	23.774025656960227	25.87807395241477	26.87405672940341	23.47384366122159
42	23.145086115056817	26.561390269886363	26.90457430752841	23.38894930752841
43	23.515735973011363	25.98960182883523	27.052723277698863	23.441938920454543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	15.0
2	23.0
3	68.5
4	114.0
5	114.0
6	148.5
7	183.0
8	168.5
9	154.0
10	195.0
11	236.0
12	236.0
13	406.0
14	576.0
15	1053.5
16	1531.0
17	1740.0
18	1949.0
19	1949.0
20	2002.5
21	2056.0
22	2291.0
23	2526.0
24	3372.0
25	4218.0
26	4218.0
27	5507.5
28	6797.0
29	8184.0
30	9571.0
31	11020.5
32	12470.0
33	12470.0
34	14928.0
35	17386.0
36	19636.0
37	21886.0
38	22962.0
39	24038.0
40	24038.0
41	24934.5
42	25831.0
43	27198.0
44	28565.0
45	28443.5
46	28322.0
47	28322.0
48	27774.5
49	27227.0
50	27598.5
51	27970.0
52	26927.0
53	25884.0
54	25884.0
55	24329.5
56	22775.0
57	20006.5
58	17238.0
59	16663.0
60	16088.0
61	16088.0
62	14865.5
63	13643.0
64	10885.0
65	8127.0
66	6674.5
67	5222.0
68	5222.0
69	4256.5
70	3291.0
71	2642.0
72	1993.0
73	1545.5
74	1098.0
75	1098.0
76	823.5
77	549.0
78	448.0
79	347.0
80	291.0
81	235.0
82	235.0
83	177.0
84	119.0
85	112.5
86	106.0
87	82.5
88	59.0
89	59.0
90	43.5
91	28.0
92	18.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	360448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.497552162642855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.59156581128823	55.6185001910984
2	6.579139201747765	8.355184692965343
3	1.90425491905447	3.6274657816089135
4	0.9626435769353667	2.445020429619462
5	0.5990397727226775	1.9018779607977967
6	0.3980291282537603	1.5164325200126645
7	0.2870530193982008	1.2759014850876973
8	0.2189142255604999	1.1120413965337923
9	0.16818324731965073	0.9611302067605968
>10	1.1511337941005368	14.021986283016485
>50	0.09627977123796568	4.087450444321602
>100	0.04113772043798563	3.9159937223006787
>500	0.0021881766190417888	0.8447786627727834
>1k	4.3763532380835775E-4	0.31623622310375904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.31571821732954547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.2344304865056818	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	617	0.1711758700284091	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	541	0.15009099786931818	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	525	0.14565207741477273	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	512	0.14204545454545456	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	489	0.13566450639204544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.548650568181819E-4	0.0	0.0	0.0	0.0
3	5.548650568181819E-4	0.0	0.0	0.0	0.0
4	5.548650568181819E-4	0.0	0.0	0.0	0.0
5	5.548650568181819E-4	0.0	0.0	0.0	0.0
6	5.548650568181819E-4	0.0	5.548650568181819E-4	0.0	0.0
7	5.548650568181819E-4	0.0	5.548650568181819E-4	0.0	0.0
8	5.548650568181819E-4	0.0	0.0013871626420454545	0.0	0.0
9	5.548650568181819E-4	0.0	0.0013871626420454545	0.0011097301136363637	0.0
10	5.548650568181819E-4	0.0	0.0013871626420454545	0.0011097301136363637	0.0
11	5.548650568181819E-4	2.7743252840909093E-4	0.0030517578125	0.0013871626420454545	0.0
12	5.548650568181819E-4	2.7743252840909093E-4	0.003329190340909091	0.0016645951704545455	0.0
13	5.548650568181819E-4	2.7743252840909093E-4	0.003329190340909091	0.0016645951704545455	0.0
14	5.548650568181819E-4	2.7743252840909093E-4	0.003606622869318182	0.0016645951704545455	0.0
15	5.548650568181819E-4	2.7743252840909093E-4	0.004161487926136364	0.0016645951704545455	0.0
16	5.548650568181819E-4	2.7743252840909093E-4	0.004161487926136364	0.0016645951704545455	0.0
17	5.548650568181819E-4	2.7743252840909093E-4	0.004438920454545455	0.0016645951704545455	0.0
18	5.548650568181819E-4	2.7743252840909093E-4	0.004438920454545455	0.0016645951704545455	0.0
19	5.548650568181819E-4	2.7743252840909093E-4	0.004716352982954545	0.0016645951704545455	0.0
20	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.0016645951704545455	0.0
21	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.0016645951704545455	0.0
22	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.0019420276988636363	0.0
23	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.002496892755681818	0.0
24	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.002496892755681818	0.0
25	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.002496892755681818	0.0
26	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.003329190340909091	0.0
27	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.004993785511363636	0.0
28	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.012761896306818182	0.0
29	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.025246360085227272	0.0
30	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.04355690696022727	0.0
31	5.548650568181819E-4	2.7743252840909093E-4	0.004993785511363636	0.08766867897727272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	25	0.005491401	29.599998	6
GACGTAT	95	1.0913936E-11	25.31579	26
GGTATCA	195	0.0	24.666668	1
GTATCAA	570	0.0	24.017544	1
GGGTATA	40	0.0019286295	23.125002	1
AGTACCG	75	3.726036E-7	22.2	5
ACGTATG	110	7.4578566E-11	21.863636	27
CAATCCA	110	7.4578566E-11	21.863636	19
ACGAGGG	60	3.7171565E-5	21.583334	15
TATATAC	70	5.0859035E-6	21.142857	3
TAGTACC	45	0.0038207902	20.555555	4
CAGACGT	120	2.3646862E-10	20.041668	24
GTGTGGG	185	0.0	20.0	1
CCCAATC	125	4.0927262E-10	19.24	17
GGCCGTA	80	1.6116579E-5	18.5	14
TATGAGT	130	6.91216E-10	18.5	30
GTGTATA	70	1.2162529E-4	18.5	1
GATTGTA	80	1.6116579E-5	18.5	23
CTCTAAT	60	9.2200533E-4	18.5	1
GGTCATG	170	0.0	18.499998	7
>>END_MODULE