FastQCFastQC Report
Fri 10 Feb 2017
ERR1633245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040797
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31460.3022683578065656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.2078215060189451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12400.11913946715834116No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11250.10809024238155951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.024.9347841
GTATCAA18000.022.5083331
TGTGACG909.476207E-820.55555510
GCGACGC500.007034446618.530
TGCGGTA1205.171387E-918.536
ATATAGG1107.8058474E-716.8181821
TTCGCCG1107.8058474E-716.81818224
AGCGACG700.002592273515.85714229
CTAGACT1201.9349754E-615.4166664
ACCGACC2800.015.1964288
GCCTAAG1101.4515272E-515.1363641
TCTAAAC1353.9707993E-715.0740753
TCTATAC1601.0957592E-815.0312513
CCGTTAA2109.094947E-1214.9761916
CGAACTA2600.014.94230729
TTAGGAC2750.014.83
ATTCCGC750.004104058314.828
TCTACGC750.004104058314.83
ATCAACG27150.014.7182323
ACCCGCA1405.9927515E-714.53571426
Produced by FastQC (version 0.11.5)