##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633245.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1040797
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876972166522386	33.0	33.0	33.0	33.0	33.0
2	31.98602128945414	33.0	33.0	33.0	33.0	33.0
3	31.703260097790444	33.0	33.0	33.0	27.0	33.0
4	31.984287041565263	33.0	33.0	33.0	33.0	33.0
5	32.0814049233424	33.0	33.0	33.0	33.0	33.0
6	35.26481244661543	37.0	37.0	37.0	33.0	37.0
7	35.4065663140843	37.0	37.0	37.0	33.0	37.0
8	35.529516322587405	37.0	37.0	37.0	33.0	37.0
9	35.64579836413825	37.0	37.0	37.0	33.0	37.0
10	35.66408435074275	37.0	37.0	37.0	33.0	37.0
11	35.68062071662389	37.0	37.0	37.0	33.0	37.0
12	35.680818641867724	37.0	37.0	37.0	33.0	37.0
13	35.681038665561104	37.0	37.0	37.0	33.0	37.0
14	35.656664075703524	37.0	37.0	37.0	33.0	37.0
15	35.66680342083999	37.0	37.0	37.0	33.0	37.0
16	35.649044914618315	37.0	37.0	37.0	33.0	37.0
17	35.64472995214244	37.0	37.0	37.0	33.0	37.0
18	35.62542263284771	37.0	37.0	37.0	33.0	37.0
19	35.61993741334766	37.0	37.0	37.0	33.0	37.0
20	35.624872093213185	37.0	37.0	37.0	33.0	37.0
21	35.63091265635854	37.0	37.0	37.0	33.0	37.0
22	35.53594312819887	37.0	37.0	37.0	33.0	37.0
23	35.583312596020164	37.0	37.0	37.0	33.0	37.0
24	35.57005448709018	37.0	37.0	37.0	33.0	37.0
25	35.591392942139535	37.0	37.0	37.0	33.0	37.0
26	35.479833243178064	37.0	37.0	37.0	33.0	37.0
27	35.499383645417886	37.0	37.0	37.0	33.0	37.0
28	35.526034375579485	37.0	37.0	37.0	33.0	37.0
29	35.53139180839299	37.0	37.0	37.0	33.0	37.0
30	35.53464988849891	37.0	37.0	37.0	33.0	37.0
31	35.52726804554587	37.0	37.0	37.0	33.0	37.0
32	35.52206530187923	37.0	37.0	37.0	33.0	37.0
33	35.51923093552345	37.0	37.0	37.0	33.0	37.0
34	35.49345165291599	37.0	37.0	37.0	33.0	37.0
35	35.445174227058686	37.0	37.0	37.0	33.0	37.0
36	35.47480728710786	37.0	37.0	37.0	33.0	37.0
37	35.47196619513699	37.0	37.0	37.0	33.0	37.0
38	35.459357588463455	37.0	37.0	37.0	33.0	37.0
39	35.36827258341444	37.0	37.0	37.0	33.0	37.0
40	35.165542368012204	37.0	37.0	37.0	33.0	37.0
41	35.346214487551364	37.0	37.0	37.0	33.0	37.0
42	35.39102341763091	37.0	37.0	37.0	33.0	37.0
43	35.07653749962769	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	43.0
16	45.0
17	38.0
18	28.0
19	33.0
20	62.0
21	183.0
22	453.0
23	1061.0
24	2176.0
25	3899.0
26	6474.0
27	9744.0
28	13814.0
29	18331.0
30	24473.0
31	31481.0
32	40226.0
33	53817.0
34	78777.0
35	162767.0
36	592868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.459765929379124	20.075480617257735	13.404919499191484	25.059833954171655
2	16.428948200273442	20.975656155811365	38.3295685902246	24.265827053690586
3	18.12178551629184	26.345098996249988	29.4049656176949	26.128149869763266
4	12.891562908040665	17.458735949469492	38.68669875105328	30.96300239143656
5	14.360917642921722	36.61895643434791	35.04333698117885	13.976788941551522
6	30.635176696320226	38.754243142514824	17.161944163943595	13.448635997221361
7	27.47394544757527	31.617404738868387	23.07068525370461	17.83796455985173
8	24.981528578579685	35.66199748846317	20.10776356964903	19.24871036330812
9	26.060509398086275	15.500525078377436	19.96950413961608	38.469461383920205
10	15.388015146085163	27.606632225112104	33.49375526639681	23.511597362405926
11	34.38730127008437	23.126700019312125	23.979604091864214	18.506394618739293
12	23.332888161668414	25.132566677267516	30.067150462578198	21.467394698485872
13	28.219047518392156	21.708171718404262	25.743636847531263	24.329143915672315
14	22.30838482432213	20.55136592438295	27.279959492581167	29.860289758713755
15	24.239116753795408	28.274870123568764	23.957025241233403	23.528987881402426
16	23.615652235738573	28.01007304978781	24.635735883174146	23.738538831299476
17	22.94895162072911	27.279094770642114	26.144003105312564	23.627950503316207
18	23.591824342306904	26.93858648708634	27.010070167381343	22.459519003225413
19	25.055606424691845	26.09346491198572	26.707897889790228	22.143030773532207
20	24.447899061968855	26.08289608828619	26.9444473802288	22.52475746951615
21	23.48104385389274	26.421290607102055	26.93186087200482	23.165804667000387
22	23.227584245534914	26.4896036402872	26.825788314147715	23.457023800030168
23	23.27562435326005	26.275921241125793	27.243737251356414	23.204717154257747
24	23.626701460515356	26.84567691874592	26.04725032835414	23.480371292384586
25	23.60104804299013	26.341928349140133	26.918121401195428	23.13890220667431
26	23.648319508991666	26.622962979332186	26.93176479178937	22.79695271988678
27	23.46768870394515	26.607205823998342	26.325402552082682	23.59970291997383
28	23.11113502440918	26.419080762146702	27.122291859027264	23.347492354416854
29	24.297821765435526	26.399480398194846	26.288219508703424	23.0144783276662
30	23.737193708283172	26.173403651240346	26.823194148330558	23.266208492145925
31	23.59701267394122	26.170233004130488	26.838759143233503	23.393995178694787
32	22.954043872147977	26.03773838702456	27.1879146461798	23.82030309464766
33	22.896299662662365	26.376228986055878	27.312146364757005	23.415324986524748
34	23.698857702318513	25.90582025121133	27.07992048401369	23.315401562456465
35	23.46826518523785	26.644388867377593	26.62843955161285	23.258906395771703
36	24.18415887055785	26.344618595172737	26.431859430801584	23.03936310346782
37	23.69856946167216	25.958568289493535	26.533224058101627	23.80963819073268
38	23.68079462181386	26.443485136871068	26.53062989228447	23.3450903490306
39	23.12852554340568	26.244118689811753	26.812721404846478	23.814634361936093
40	23.43021741991954	26.696272183720744	26.915815476024623	22.95769492033509
41	23.30396801681788	25.94972890967211	27.008148563072336	23.738154510437674
42	23.187903116553947	26.709723413883786	26.82357846919236	23.278795000369907
43	23.725279761567336	25.850862367973775	26.732110104083695	23.69174776637519
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	35.0
2	46.0
3	174.0
4	302.0
5	302.0
6	387.0
7	472.0
8	419.5
9	367.0
10	535.5
11	704.0
12	704.0
13	1129.5
14	1555.0
15	2847.5
16	4140.0
17	4804.0
18	5468.0
19	5468.0
20	5298.0
21	5128.0
22	5830.0
23	6532.0
24	9153.5
25	11775.0
26	11775.0
27	15397.0
28	19019.0
29	23204.0
30	27389.0
31	32569.0
32	37749.0
33	37749.0
34	45768.0
35	53787.0
36	58743.0
37	63699.0
38	67526.5
39	71354.0
40	71354.0
41	74426.5
42	77499.0
43	79717.5
44	81936.0
45	80765.0
46	79594.0
47	79594.0
48	77859.0
49	76124.0
50	76040.5
51	75957.0
52	72865.0
53	69773.0
54	69773.0
55	67132.5
56	64492.0
57	58266.5
58	52041.0
59	48676.0
60	45311.0
61	45311.0
62	40366.5
63	35422.0
64	30038.5
65	24655.0
66	21089.5
67	17524.0
68	17524.0
69	14964.5
70	12405.0
71	10112.5
72	7820.0
73	6253.5
74	4687.0
75	4687.0
76	3651.5
77	2616.0
78	2030.0
79	1444.0
80	1143.5
81	843.0
82	843.0
83	697.0
84	551.0
85	434.0
86	317.0
87	241.5
88	166.0
89	166.0
90	125.5
91	85.0
92	54.5
93	24.0
94	12.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1040797.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.11370164366414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.58463009461283	52.53864477336477
2	9.211883889862609	11.443684150220047
3	2.5334354866824045	4.720831678597858
4	1.0873845856532782	2.701659269007483
5	0.6177032403959085	1.9183917389138005
6	0.3777563860588533	1.4078308474589054
7	0.2703313261157554	1.1753895534703072
8	0.177348640680087	0.8812624443289858
9	0.1387106833831634	0.7754250602205516
>10	0.8604186859369787	10.026294790547391
>50	0.07996924891311585	3.475966227254944
>100	0.05558726815295721	6.472027391186056
>500	0.004059743624314555	1.6198736892526315
>1k	7.80719927752799E-4	0.8427183861762731
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3146	0.3022683578065656	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2163	0.2078215060189451	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1240	0.11913946715834116	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1125	0.10809024238155951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.9216043090055024E-4	0.0	0.0
2	1.9216043090055024E-4	0.0	1.9216043090055024E-4	0.0	0.0
3	1.9216043090055024E-4	0.0	3.843208618011005E-4	9.608021545027512E-5	0.0
4	1.9216043090055024E-4	0.0	3.843208618011005E-4	2.882406463508254E-4	0.0
5	1.9216043090055024E-4	0.0	4.8040107725137564E-4	3.843208618011005E-4	0.0
6	1.9216043090055024E-4	0.0	5.764812927016508E-4	4.8040107725137564E-4	0.0
7	1.9216043090055024E-4	0.0	0.001537283447204402	4.8040107725137564E-4	0.0
8	1.9216043090055024E-4	0.0	0.0020176845244557775	4.8040107725137564E-4	0.0
9	1.9216043090055024E-4	0.0	0.0025941658171574284	0.0010568823699530264	0.0
10	3.843208618011005E-4	0.0	0.003266727325309354	0.0012490428008535767	0.0
11	3.843208618011005E-4	0.0	0.004900090987964031	0.0013451230163038519	0.0
12	3.843208618011005E-4	0.0	0.005572652496115957	0.0013451230163038519	0.0
13	3.843208618011005E-4	0.0	0.005956973357917058	0.0014412032317541268	0.0
14	3.843208618011005E-4	0.0	0.006245214004267884	0.0014412032317541268	0.0
15	3.843208618011005E-4	0.0	0.006725615081519259	0.001537283447204402	0.0
16	3.843208618011005E-4	0.0	0.007013855727870084	0.001537283447204402	0.0
17	3.843208618011005E-4	0.0	0.007206016158770635	0.001537283447204402	0.0
18	4.8040107725137564E-4	0.0	0.00730209637422091	0.0017294438781049522	0.0
19	4.8040107725137564E-4	0.0	0.0074942568051214594	0.0019216043090055025	0.0
20	4.8040107725137564E-4	0.0	0.007782497451472285	0.0022098449553563278	0.0
21	4.8040107725137564E-4	0.0	0.00807073809782311	0.002305925170806603	0.0
22	4.8040107725137564E-4	0.0	0.008166818313273386	0.0027863262480579787	0.0
23	4.8040107725137564E-4	0.0	0.008166818313273386	0.002978486678958529	0.0
24	4.8040107725137564E-4	0.0	0.008166818313273386	0.00393928883346128	0.0
25	5.764812927016508E-4	0.0	0.008166818313273386	0.004323609695262381	0.0
26	5.764812927016508E-4	0.0	0.008166818313273386	0.0045157701261629305	0.0
27	5.764812927016508E-4	0.0	0.008166818313273386	0.005092251418864582	0.0
28	6.725615081519259E-4	0.0	0.008166818313273386	0.012202187362184942	0.0
29	6.725615081519259E-4	0.0	0.008166818313273386	0.025749497740673734	0.0
30	6.725615081519259E-4	0.0	0.008166818313273386	0.04467730018437793	0.0
31	6.725615081519259E-4	0.0	0.008166818313273386	0.09704101760477787	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	690	0.0	24.934784	1
GTATCAA	1800	0.0	22.508333	1
TGTGACG	90	9.476207E-8	20.555555	10
GCGACGC	50	0.0070344466	18.5	30
TGCGGTA	120	5.171387E-9	18.5	36
ATATAGG	110	7.8058474E-7	16.818182	1
TTCGCCG	110	7.8058474E-7	16.818182	24
AGCGACG	70	0.0025922735	15.857142	29
CTAGACT	120	1.9349754E-6	15.416666	4
ACCGACC	280	0.0	15.196428	8
GCCTAAG	110	1.4515272E-5	15.136364	1
TCTAAAC	135	3.9707993E-7	15.074075	3
TCTATAC	160	1.0957592E-8	15.031251	3
CCGTTAA	210	9.094947E-12	14.97619	16
CGAACTA	260	0.0	14.942307	29
TTAGGAC	275	0.0	14.8	3
ATTCCGC	75	0.0041040583	14.8	28
TCTACGC	75	0.0041040583	14.8	3
ATCAACG	2715	0.0	14.718232	3
ACCCGCA	140	5.9927515E-7	14.535714	26
>>END_MODULE