FastQCFastQC Report
Fri 10 Feb 2017
ERR1633244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036771
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136741.3189026313428907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96720.93289646411792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53680.5177613957180516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.2265688372842219No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22870.22058873174500443No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19180.18499745845514584No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18730.18065705927345577No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16430.15847279678926204No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15140.14603031913508385No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14180.13677080088081167No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.13599917435962233No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13030.12567866963871482No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG11680.1126574720936446No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11480.11072840579067122No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11340.10937805937858987No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10900.10513411351204846No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10410.10040790106976372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.023.9872881
GTATCAA61100.022.4967271
CGCTCTC4300.019.36046429
CCGCTCT4500.018.91111228
GCCGCTC4650.018.69892527
ACTAGTG1501.2732926E-1118.58
TTCGCCG4700.018.524
AGCAACG500.007034429318.4999982
TTAACGG1950.018.02564235
CATACTA2800.017.8392855
TCATACT3050.017.5901624
GCCGGCA5050.017.58415815
ACGTATA953.6056972E-617.52631629
GCCGTCC3700.017.527
TGCGTCG752.0670205E-417.26666610
GTGCGGC650.00157979317.0769239
AGCTTCG5550.017.00000221
CCGGCAG5150.016.88349516
CGCCGTC3850.016.81818426
AACGGCC2100.016.73809437
Produced by FastQC (version 0.11.5)