##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1036771
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94469174002745	33.0	33.0	33.0	33.0	33.0
2	32.05026664518973	33.0	33.0	33.0	33.0	33.0
3	31.7680606421283	33.0	33.0	33.0	33.0	33.0
4	32.03317704681169	33.0	33.0	33.0	33.0	33.0
5	32.135009563346195	33.0	33.0	33.0	33.0	33.0
6	35.30061604732386	37.0	37.0	37.0	33.0	37.0
7	35.457709561706494	37.0	37.0	37.0	33.0	37.0
8	35.5959020844526	37.0	37.0	37.0	33.0	37.0
9	35.71038831140146	37.0	37.0	37.0	33.0	37.0
10	35.70438987973236	37.0	37.0	37.0	33.0	37.0
11	35.73244139737705	37.0	37.0	37.0	33.0	37.0
12	35.71431685492746	37.0	37.0	37.0	33.0	37.0
13	35.71096317315974	37.0	37.0	37.0	33.0	37.0
14	35.69943121480057	37.0	37.0	37.0	33.0	37.0
15	35.72445699194904	37.0	37.0	37.0	33.0	37.0
16	35.70552706431796	37.0	37.0	37.0	33.0	37.0
17	35.70688319792895	37.0	37.0	37.0	33.0	37.0
18	35.68218536205199	37.0	37.0	37.0	33.0	37.0
19	35.676806160666146	37.0	37.0	37.0	33.0	37.0
20	35.67335988371588	37.0	37.0	37.0	33.0	37.0
21	35.672379918033975	37.0	37.0	37.0	33.0	37.0
22	35.57200288202505	37.0	37.0	37.0	33.0	37.0
23	35.61403723676685	37.0	37.0	37.0	33.0	37.0
24	35.61274090421125	37.0	37.0	37.0	33.0	37.0
25	35.64046448058443	37.0	37.0	37.0	33.0	37.0
26	35.53426552247314	37.0	37.0	37.0	33.0	37.0
27	35.548756668541074	37.0	37.0	37.0	33.0	37.0
28	35.56172674582912	37.0	37.0	37.0	33.0	37.0
29	35.558810962112176	37.0	37.0	37.0	33.0	37.0
30	35.56191675885996	37.0	37.0	37.0	33.0	37.0
31	35.544396014163205	37.0	37.0	37.0	33.0	37.0
32	35.51934323008649	37.0	37.0	37.0	33.0	37.0
33	35.50411035802506	37.0	37.0	37.0	33.0	37.0
34	35.46469760438901	37.0	37.0	37.0	33.0	37.0
35	35.39413621715885	37.0	37.0	37.0	33.0	37.0
36	35.41310569064914	37.0	37.0	37.0	33.0	37.0
37	35.37967593615176	37.0	37.0	37.0	33.0	37.0
38	35.337473752641614	37.0	37.0	37.0	33.0	37.0
39	35.22464748724646	37.0	37.0	37.0	33.0	37.0
40	34.97822759317149	37.0	37.0	37.0	27.0	37.0
41	35.119612720649016	37.0	37.0	37.0	33.0	37.0
42	35.116939999286245	37.0	37.0	37.0	33.0	37.0
43	34.753072761487346	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	27.0
16	69.0
17	50.0
18	45.0
19	34.0
20	72.0
21	145.0
22	420.0
23	1041.0
24	1975.0
25	3542.0
26	6097.0
27	9376.0
28	13646.0
29	18636.0
30	25092.0
31	32036.0
32	41514.0
33	54427.0
34	78831.0
35	159249.0
36	590441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27840960057718	19.50305322969103	14.367396464600187	22.851140705131606
2	16.07635630240429	22.523874606832177	38.99414624830363	22.40562284245991
3	18.240382881079814	28.392287207107454	30.306596152863076	23.060733758949663
4	12.00747320285772	18.241058054285855	39.91074210216142	29.840726640695003
5	14.11951144466811	36.510762743170865	33.8139280516141	15.55579776054693
6	27.606289141960954	40.73252434722808	16.962665815305407	14.698520695505565
7	25.84350835430389	33.35027696569445	22.378230100957687	18.427984579043972
8	25.172482640814607	33.764351047627684	20.23744877123299	20.82571754032472
9	27.658277478826086	14.361705719006416	19.745343957344485	38.23467284482302
10	16.117445414657624	27.33168655373269	32.100627814628304	24.450240216981378
11	33.95098821244036	24.302184378228173	22.94064938158957	18.8061780277419
12	24.027485336684766	26.391556090978625	28.95412776784844	20.626830804488165
13	29.69025946906308	22.81101612602976	25.366932524154322	22.13179188075284
14	23.11850929472371	21.37173975738133	28.881016154965756	26.628734792929198
15	25.071881833114546	27.456690050165367	25.901959063283986	21.5694690534361
16	21.497997146910937	28.473018631886887	25.809074520795818	24.219909700406358
17	22.23673308763459	26.412197100420443	26.10296777205381	25.24810203989116
18	24.418699982927762	24.58189899215931	28.444275543972587	22.555125480940344
19	26.010083229565637	24.887077281289695	28.78880678568363	20.314032703461034
20	25.450075281812474	24.05294901188401	28.830860431088446	21.666115275215066
21	23.15776579398922	25.969572837203202	28.984028295544533	21.888633073263044
22	23.032376484295952	25.68792915696909	28.834429203748947	22.44526515498601
23	22.502847784129763	25.62851391483751	29.745623671958416	22.12301462907431
24	22.636917892186414	25.918645486804703	28.74887511321208	22.695561507796803
25	22.612901016714396	25.85247851261272	29.864164796276132	21.670455674396756
26	22.909205600851106	26.00718963011118	28.998014026241087	22.085590742796626
27	23.161816833225465	25.57941917742684	29.174523592963155	22.084240396384544
28	22.18956741652689	25.855372112067176	29.08202486373558	22.873035607670353
29	23.37517156633432	25.79007321771153	28.322647913570115	22.512107302384038
30	23.331478214571973	25.485280741841738	29.096203501062433	22.087037542523856
31	23.014050354417705	25.534375479252407	29.009009704168037	22.442564462161847
32	21.571494573054224	25.610766504850158	29.444978688640017	23.3727602334556
33	21.713184493007617	25.70741272662912	29.822496964131908	22.756905816231356
34	22.13381740037096	25.67413633290283	29.28486618549323	22.90718008123298
35	22.07864610410592	26.46669322347944	29.072765345481304	22.381895326933336
36	22.81381327216907	26.004488937287018	29.055596655384846	22.126101135159065
37	22.968620842982684	25.98934576680868	28.700166189062	22.341867201146638
38	22.776389385891388	26.15948941473093	28.135817842127143	22.92830335725054
39	22.283223585536245	25.726317576398262	28.711065413673804	23.279393424391692
40	22.498989651523818	26.6651941460554	28.836165363421625	21.999650838999163
41	22.726715928589826	25.593597814753693	28.574873332683882	23.104812923972602
42	21.718971691916536	26.731071760301937	28.680103899511078	22.86985264827045
43	22.57904590309721	26.156113548700727	28.273553176159442	22.99128737204262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	92.5
2	151.0
3	609.0
4	1067.0
5	1067.0
6	1384.0
7	1701.0
8	1666.0
9	1631.0
10	2243.0
11	2855.0
12	2855.0
13	4623.0
14	6391.0
15	11571.5
16	16752.0
17	18421.5
18	20091.0
19	20091.0
20	16401.5
21	12712.0
22	10877.5
23	9043.0
24	10602.5
25	12162.0
26	12162.0
27	14741.0
28	17320.0
29	20210.5
30	23101.0
31	26897.0
32	30693.0
33	30693.0
34	37251.5
35	43810.0
36	46848.0
37	49886.0
38	54077.5
39	58269.0
40	58269.0
41	62042.5
42	65816.0
43	69492.5
44	73169.0
45	77871.0
46	82573.0
47	82573.0
48	84201.5
49	85830.0
50	88539.0
51	91248.0
52	88109.0
53	84970.0
54	84970.0
55	77266.0
56	69562.0
57	61128.0
58	52694.0
59	47546.5
60	42399.0
61	42399.0
62	35869.0
63	29339.0
64	23610.0
65	17881.0
66	15667.0
67	13453.0
68	13453.0
69	10936.5
70	8420.0
71	6807.0
72	5194.0
73	4269.0
74	3344.0
75	3344.0
76	2387.5
77	1431.0
78	1073.0
79	715.0
80	572.0
81	429.0
82	429.0
83	351.5
84	274.0
85	225.5
86	177.0
87	138.0
88	99.0
89	99.0
90	82.0
91	65.0
92	39.5
93	14.0
94	9.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1036771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.201515816469225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.86144202769337	40.42248624289779
2	9.241220859247951	8.908817068210508
3	2.494810701852129	3.607609725132662
4	1.0686308852656925	2.0603851407240716
5	0.6576964811645578	1.5850983669644794
6	0.40929686458011943	1.1837237575013932
7	0.30050158843467445	1.013924244746566
8	0.23756701532102056	0.9160872197174048
9	0.15865171483162005	0.6882527827589668
>10	1.2554693452497578	12.046982109512932
>50	0.16294552317042738	5.55658538874293
>100	0.13388815064287984	12.510213866318121
>500	0.014221806570492986	4.5043076595374165
>1k	0.003047529979391354	2.1835536011459022
>5k	4.0633733058551393E-4	1.4728728600813727
>10k+	2.0316866529275696E-4	1.3390999660074927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13674	1.3189026313428907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9672	0.93289646411792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5368	0.5177613957180516	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2349	0.2265688372842219	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2287	0.22058873174500443	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1918	0.18499745845514584	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1873	0.18065705927345577	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1643	0.15847279678926204	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1514	0.14603031913508385	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1418	0.13677080088081167	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1410	0.13599917435962233	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1303	0.12567866963871482	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	1168	0.1126574720936446	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1148	0.11072840579067122	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1134	0.10937805937858987	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1090	0.10513411351204846	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1041	0.10040790106976372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.8581326059467327E-4	9.645331514866832E-5	0.0
2	2.8935994544600495E-4	0.0	5.787198908920099E-4	9.645331514866832E-5	0.0
3	2.8935994544600495E-4	0.0	7.716265211893465E-4	1.9290663029733663E-4	0.0
4	2.8935994544600495E-4	0.0	9.645331514866832E-4	1.9290663029733663E-4	0.0
5	4.822665757433416E-4	0.0	0.001543253042378693	1.9290663029733663E-4	0.0
6	4.822665757433416E-4	0.0	0.004533305811987411	1.9290663029733663E-4	0.0
7	4.822665757433416E-4	0.0	0.012056664393583539	2.8935994544600495E-4	0.0
8	4.822665757433416E-4	0.0	0.014082184011705575	2.8935994544600495E-4	0.0
9	4.822665757433416E-4	0.0	0.017650956672206303	0.001543253042378693	0.0
10	0.0019290663029733663	0.0	0.02015874286607168	0.0019290663029733663	0.0
11	0.002025519618122035	0.0	0.029514714435492504	0.002121972933270703	0.0
12	0.002025519618122035	0.0	0.03192604731420921	0.002121972933270703	0.0
13	0.002025519618122035	0.0	0.034433833508074586	0.002121972933270703	0.0
14	0.002025519618122035	0.0	0.03607353986560195	0.0022184262484193714	0.0
15	0.002025519618122035	0.0	0.03916004595035934	0.0023148795635680396	0.0
16	0.002121972933270703	0.0	0.04186073877452205	0.002411332878716708	0.0
17	0.002121972933270703	0.0	0.04282527192600873	0.002507786193865376	0.0
18	0.002121972933270703	0.0	0.044368524968387425	0.002700692824162713	0.0
19	0.0022184262484193714	0.0	0.04514015148957677	0.0028935994544600496	0.0
20	0.0022184262484193714	0.0	0.046297591271360794	0.0032794127150547227	0.0
21	0.0023148795635680396	0.0	0.04774439099859082	0.003954585921095401	0.0
22	0.0023148795635680396	0.0	0.048901830780374834	0.0047262124422847475	0.0
23	0.0023148795635680396	0.0	0.04938409735611818	0.006365918799812109	0.0
24	0.0023148795635680396	0.0	0.049480550671266846	0.00810207847248814	0.0
25	0.0023148795635680396	0.0	0.04957700398641551	0.008777251678528816	0.0
26	0.002411332878716708	0.0	0.04957700398641551	0.010609864666353514	0.0
27	0.002411332878716708	0.0	0.049769910616712855	0.01379282406625957	0.0
28	0.002507786193865376	9.645331514866832E-5	0.04996281724701019	0.0313473274233172	0.0
29	0.002507786193865376	9.645331514866832E-5	0.04996281724701019	0.07465486592506927	0.0
30	0.002507786193865376	9.645331514866832E-5	0.04996281724701019	0.12895808235376954	0.0
31	0.002507786193865376	9.645331514866832E-5	0.04996281724701019	0.23081278315076328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2360	0.0	23.987288	1
GTATCAA	6110	0.0	22.496727	1
CGCTCTC	430	0.0	19.360464	29
CCGCTCT	450	0.0	18.911112	28
GCCGCTC	465	0.0	18.698925	27
ACTAGTG	150	1.2732926E-11	18.5	8
TTCGCCG	470	0.0	18.5	24
AGCAACG	50	0.0070344293	18.499998	2
TTAACGG	195	0.0	18.025642	35
CATACTA	280	0.0	17.839285	5
TCATACT	305	0.0	17.590162	4
GCCGGCA	505	0.0	17.584158	15
ACGTATA	95	3.6056972E-6	17.526316	29
GCCGTCC	370	0.0	17.5	27
TGCGTCG	75	2.0670205E-4	17.266666	10
GTGCGGC	65	0.001579793	17.076923	9
AGCTTCG	555	0.0	17.000002	21
CCGGCAG	515	0.0	16.883495	16
CGCCGTC	385	0.0	16.818184	26
AACGGCC	210	0.0	16.738094	37
>>END_MODULE