FastQCFastQC Report
Fri 10 Feb 2017
ERR1633243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895541
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263482.9421321860194007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197962.2105073916213773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109191.219262992984129No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.5325272656416624No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA43140.48171998825291085No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG40270.44967232097692905No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA37460.41829463977640335No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG35610.39763673578317466No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA31970.35699091387217335No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA28280.3157867702316254No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.3104268816279768No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT26690.29803213923203964No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA25000.2791608647733605No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG24150.2696693953710662No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23660.26419784242150834No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC22880.25548802344057947No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT21120.2358350985605349No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC20970.23416013337189476No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19720.22020209013322672No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC19680.21975543274958936No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19530.2180804675609492No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18520.20680236862410545No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA17780.19853920702681396No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA17540.19585926272498969No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17470.19507761230362428No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17410.1944076262281682No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC17170.19172768192634398No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17100.19094603150497855No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG16930.1890477376245197No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC16680.1862561289767861No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16530.18458116378814593No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.1794446038763161No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA15460.1726330787758461No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15180.16950647709038447No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA15130.16894815536083774No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14940.16682653278856022No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA14520.16213663026036776No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14380.16057332941763694No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14370.1604616650717276No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14270.15934502161263417No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14220.1587866998830874No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC14050.15688840600262857No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA13980.1561067555812632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.15398513300898564No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT13750.15353847562534825No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT13710.1530918182417109No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG13590.15175184609079873No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA13540.15119352436125202No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13430.14996521655624923No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13410.14974188786443055No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13390.14951855917261186No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13010.14527531402805677No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.14460532795260073No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT12870.14371201318532598No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12770.14259536972623255No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG12580.140473747153955No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG12410.13857545327349613No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG12300.13734714546849333No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT11960.13355055770757565No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG11850.13232224990257285No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG11820.13198725686484483No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG11660.13020062733029533No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11590.1294189769089299No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC11490.12830233344983646No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA11440.12774401172028974No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT11190.12495240307255613No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG11190.12495240307255613No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG11020.12305410919209729No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT10980.1226074518084599No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10950.1222724587707319No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10940.12216079442482254No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10640.11881086404754221No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG10600.11836420666390483No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC10390.11601925539980862No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC10150.11333931109798435No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10130.11311598240616566No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10060.11233433198480024No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.11110602417979745No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9820.10965438768297597No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA9750.10887273726161058No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT9740.10876107291570122No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9660.10786775814842649No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG9530.106416121651605No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA9450.10552280688433024No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA9410.10507614950069288No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA9160.10228454085295927No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA9160.10228454085295927No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9120.10183788346932189No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9000.10049791131840978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAA200.001841718637.01
CTAGTCG251.2334078E-437.09
TAACAAC501.8007995E-1037.01
TGACTCT251.2334078E-437.033
GTAGCTT250.00549570329.619
ATAAGCC250.00549570329.63
TCTCAAC250.00549570329.62
GATTAGT250.00549570329.61
CTAGTGC1150.028.9565229
CTTCCCT454.006957E-628.77777735
GACCCAA556.259161E-726.9090935
TCGCTAA556.259161E-726.9090931
CTCGCTA556.259161E-726.9090930
GGACCCA556.259161E-726.9090934
AACAACG706.5883796E-926.428572
ACAACGC809.786163E-1025.4375023
ACTAGTG2400.025.4374988
CGCTCTC10350.024.84541129
CCGCTCT10450.024.78468928
TAGTGCA1350.024.66666810
Produced by FastQC (version 0.11.5)