FastQCFastQC Report
Fri 10 Feb 2017
ERR1633242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867875
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214072.4665994526861588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT154421.7792884920063374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86120.992308800230448No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA52380.6035431369724903No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA44300.5104421719717702No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG41280.47564453406308516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.41457583177300883No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA34840.4014402995823131No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT33520.38623073599308655No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG33020.38046953766383407No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30080.3465936914878295No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT25070.2888664842287196No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC23480.2705458735416967No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23410.26973930577560135No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT22820.2629410917470834No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC22700.2615584041480628No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG21660.24957511162321763No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG21290.24531182485957082No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21240.24473570502664554No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21140.24358346536079503No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC21080.24289212156128476No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC20600.23736137116520234No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20310.23401987613423594No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA19760.2276825579720582No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19660.2265303183062077No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA19530.22503240674060207No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA18300.2108598588506409No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA18150.20913149935186517No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA17550.20221806135676224No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA17330.19968313409189112No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT17050.19645686302750973No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16870.19438283162897882No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16560.19081088866484228No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC16500.19011954486533197No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT16400.1889673051994815No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC16380.1887368572663114No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC16070.18516491430217485No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG15980.1841278986029094No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG15930.18355177876998416No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG15700.180901627538528No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC15510.17871237217341207No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15310.17640789284171107No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT15200.17514042920927553No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14730.1697249027797782No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14700.16937923088002305No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA14360.16546161601613135No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14200.16361803255077056No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG14130.1628114647846752No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG14040.16177444908540975No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG13820.15923952182053866No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT13790.15889384992078354No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG13790.15889384992078354No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA13570.15635892265591242No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG13480.15532190695664697No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA13260.15278697969177588No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13120.1511738441595852No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13110.15105862019300015No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12950.14921503672763936No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12920.1488693648278842No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12860.1481780210283739No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12490.14391473426472706No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11980.13803831196888952No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.13654040040328388No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA11730.13515771280426328No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT11680.13458159297133804No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC11670.13446636900475298No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG11600.13365980123865764No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11530.1328532334725623No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11490.1323923376062221No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11390.1312400979403716No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA11380.13112487397378655No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC11360.13089442604061646No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG11270.129857410341351No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA11150.1284747227423304No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC11020.12697681117672477No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG10910.12570934754428922No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG10880.12536367564453407No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10640.12259830044649288No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10500.12098516491430217No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10470.12063949301454702No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG10430.12017859714820683No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.11879590954918624No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC10270.11833501368284603No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10180.11729799798358058No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA10060.11591531038455999No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC9990.11510874261846464No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG9900.11407172691919919No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG9870.11372605501944405No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG9850.11349560708627394No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT9850.11349560708627394No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG9820.11314993518651881No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT9810.11303471121993373No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG9690.11165202362091314No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9600.1106150079216477No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9390.10819530462336166No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG9310.10727351289068125No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9170.10566037735849057No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA9130.10519948149215036No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1041624657928849No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA8890.10243410629410918No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8890.10243410629410918No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8880.10231888232752413No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG8840.10185798646118394No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8700.10024485092899323No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8700.10024485092899323No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8680.10001440299582313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCTA352.3876391E-531.71428520
TATTATA601.2696546E-930.8333342
ACAACGC502.71948E-729.5999983
CTAGTCG250.00549561129.5999989
TCCAACT250.00549561129.59999826
TTGGGCT250.00549561129.59999820
CTCCACT454.00677E-628.7777772
TAACAAC454.00677E-628.7777771
GTATTAT706.5883796E-926.428571
GGCGTTC358.8676374E-426.4285712
AACAACG509.087738E-625.8999982
TATAGAT659.386349E-825.6153855
ATTATAG659.386349E-825.6153853
CTAGTGC1051.8189894E-1224.6666689
GTGATCT451.3228472E-424.66666619
GGTATCA37500.024.0746651
TTATAGA701.9217987E-723.7857134
TAGACCG400.001930808423.1255
TATGCGT802.7239366E-823.1253
AAACCCG400.001930808423.12512
Produced by FastQC (version 0.11.5)