FastQCFastQC Report
Fri 10 Feb 2017
ERR1633241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848361
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25650.30234770339513484No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC21960.25885206887162426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.2147670626066026No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC18010.21229170129225647No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG17170.20239025603487193No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16440.19378542860881157No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15990.1884810829352127No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA15890.18730233945219074No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13470.15877674716305912No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13280.15653713454531737No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12300.1449854484117021No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10940.12895453704260332No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10790.12718642181807038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10730.12647917572825718No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10720.126361301379955No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10340.12188207614447152No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10230.12058545831314736No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9920.11693135351577925No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9800.1155168613361529No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA9760.11504536394294411No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG9600.11315937437010896No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT9540.11245212828029577No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG9330.10997676696594964No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT9180.10820865174141668No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9180.10820865174141668No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8960.10561541607876837No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8870.10455454694404857No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8850.1043187982474442No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT8840.104200923899142No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT8760.10325792911272441No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8740.10302218041612003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC303.5986904E-430.8333348
GGTATCA5950.023.9411771
TTAGACT802.7237547E-823.1250024
AACGATT1301.8189894E-1221.34615322
GTATCAA14300.020.9580421
GTCCGGC555.141569E-420.18181815
CATGCTA5000.019.984
TTGCGGT951.6748163E-719.47368435
CGTGTCG951.6748163E-719.4736849
ACGATTA1355.638867E-1119.18518423
TACGTGA1355.638867E-1119.18518436
TACATGC5100.018.8627452
GTGTTAC701.2187194E-418.51
ACCTCGG1501.2732926E-1118.536
GTCAACC5100.018.515
GGACCGT1002.8727118E-718.56
TAGGACC2500.018.54
GTTATCG500.007033418.511
CGAACTA5250.018.3238129
CAACCCA5100.018.13725517
Produced by FastQC (version 0.11.5)