##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633241.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	848361
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.876986330111826	33.0	33.0	33.0	33.0	33.0
2	31.995227267637244	33.0	33.0	33.0	33.0	33.0
3	31.707345104265755	33.0	33.0	33.0	27.0	33.0
4	31.995828426813585	33.0	33.0	33.0	33.0	33.0
5	32.09244413639948	33.0	33.0	33.0	33.0	33.0
6	35.28550463776623	37.0	37.0	37.0	33.0	37.0
7	35.40616789314926	37.0	37.0	37.0	33.0	37.0
8	35.53110409365824	37.0	37.0	37.0	33.0	37.0
9	35.64936742730983	37.0	37.0	37.0	33.0	37.0
10	35.66021068861016	37.0	37.0	37.0	33.0	37.0
11	35.6785083236971	37.0	37.0	37.0	33.0	37.0
12	35.6662269953475	37.0	37.0	37.0	33.0	37.0
13	35.67355524358145	37.0	37.0	37.0	33.0	37.0
14	35.64188358493613	37.0	37.0	37.0	33.0	37.0
15	35.65776951085682	37.0	37.0	37.0	33.0	37.0
16	35.64136022282967	37.0	37.0	37.0	33.0	37.0
17	35.63205168554424	37.0	37.0	37.0	33.0	37.0
18	35.61877785518193	37.0	37.0	37.0	33.0	37.0
19	35.6187177392643	37.0	37.0	37.0	33.0	37.0
20	35.619013603878535	37.0	37.0	37.0	33.0	37.0
21	35.61170303679683	37.0	37.0	37.0	33.0	37.0
22	35.51989542187819	37.0	37.0	37.0	33.0	37.0
23	35.582292208151955	37.0	37.0	37.0	33.0	37.0
24	35.55825880727662	37.0	37.0	37.0	33.0	37.0
25	35.58588619703169	37.0	37.0	37.0	33.0	37.0
26	35.48447535895686	37.0	37.0	37.0	33.0	37.0
27	35.49532922894853	37.0	37.0	37.0	33.0	37.0
28	35.520884387660445	37.0	37.0	37.0	33.0	37.0
29	35.528814973814214	37.0	37.0	37.0	33.0	37.0
30	35.521959401716956	37.0	37.0	37.0	33.0	37.0
31	35.508069088513025	37.0	37.0	37.0	33.0	37.0
32	35.51220647813843	37.0	37.0	37.0	33.0	37.0
33	35.52075944085124	37.0	37.0	37.0	33.0	37.0
34	35.50131488835531	37.0	37.0	37.0	33.0	37.0
35	35.438292189292056	37.0	37.0	37.0	33.0	37.0
36	35.463235580136285	37.0	37.0	37.0	33.0	37.0
37	35.46133544564165	37.0	37.0	37.0	33.0	37.0
38	35.45928914695513	37.0	37.0	37.0	33.0	37.0
39	35.37011366623407	37.0	37.0	37.0	33.0	37.0
40	35.15432109679723	37.0	37.0	37.0	33.0	37.0
41	35.32833546096532	37.0	37.0	37.0	33.0	37.0
42	35.384924578098236	37.0	37.0	37.0	33.0	37.0
43	35.06396805133664	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	23.0
16	39.0
17	32.0
18	29.0
19	27.0
20	56.0
21	155.0
22	353.0
23	879.0
24	1771.0
25	3263.0
26	5253.0
27	7891.0
28	11099.0
29	15243.0
30	20019.0
31	25696.0
32	33141.0
33	44294.0
34	64288.0
35	132331.0
36	482476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.188715652888334	20.59241289969718	13.2049917429019	25.013879704512583
2	16.42154695937225	20.82509686324572	39.024542618059996	23.728813559322035
3	17.543710755209162	27.265515505781146	29.443833462405745	25.746940276603947
4	12.413936991445858	17.567639247914506	39.842472720928946	30.17595103971069
5	13.554135562573007	37.51634033153339	35.75235070919101	13.177173396702582
6	29.965898951036174	39.85225629183803	17.037911926644437	13.143932830481365
7	27.19361215331681	31.678141734473886	23.78315363388935	17.34509247831996
8	24.45303355529073	36.31802970669326	20.185510649357997	19.043426088658013
9	25.992354669769117	15.443189868464014	20.260007237484988	38.30444822428188
10	15.461578266799158	27.84298193811361	33.777955375129224	22.91748441995801
11	33.91362875002505	23.58335661351712	23.87120577207109	18.63180886438674
12	23.57569478087748	26.030545958619033	29.20490215839719	21.188857102106297
13	26.930634482254607	22.178294381754938	26.184371983153397	24.706699152837057
14	21.844474227363115	20.675867938295138	27.55065355432416	29.929004280017583
15	24.26408097496231	28.377777856360677	24.698330074107602	22.65981109456941
16	23.24729684650756	28.12081177706189	24.980285515246457	23.651605861184095
17	22.40956385312385	27.640238059033834	26.061193289177602	23.889004798664722
18	22.558085531984613	27.64106317947195	27.836263100260382	21.964588188283056
19	24.93537538854332	26.038915037348488	27.010199667358588	22.015509906749603
20	23.801424157876188	26.503104220962538	27.61430570240735	22.081165918753925
21	23.28442726622275	27.058999647555698	27.298048825912552	22.358524260308997
22	22.868330816715996	27.090472098552386	26.951616116252396	23.089580968479222
23	22.885658345916422	26.95656683888109	27.731472804619735	22.42630201058276
24	23.431652327252195	27.660748195638412	25.601483330799034	23.30611614631036
25	23.010487280768448	27.063007375397973	27.466137646591488	22.460367697242095
26	23.25047945391172	27.240997641334292	27.641181053820247	21.86734185093374
27	23.122821534700442	27.172748393667316	26.103745928914694	23.600684142717547
28	22.282848928698986	26.616852967074156	28.225484198354238	22.874813905872617
29	24.59790112935413	26.41139797798343	26.621567941006248	22.369132951656194
30	23.47043298784362	26.35399317036026	27.472267112703204	22.70330672909292
31	22.892848681162853	26.76278141027228	27.524013951607866	22.820355956957002
32	21.91248772633348	26.428961255880456	27.785812879187045	23.87273813859902
33	22.170632549115293	26.71657466573782	28.073897786437612	23.038894998709274
34	23.411142190647613	26.55779791857476	27.855594493381947	22.175465397395683
35	23.360220472181066	27.209289441640998	27.0429687361866	22.387521349991335
36	24.266556336276658	26.974247991126422	26.23211109421579	22.52708457838114
37	22.856071884492568	26.30955454105033	27.01880449478465	23.81556907967245
38	23.556127639059316	26.738027797128815	26.874644166811063	22.831200397000806
39	22.411449842696683	26.961046064116573	27.423585006854395	23.203919086332352
40	22.841691213999702	27.526253564225605	27.485115416668137	22.146939805106552
41	22.469444022061364	26.100091824117328	27.6922206466351	23.738243507186212
42	22.147175553803155	27.635640959450047	27.188543556339813	23.028639930406985
43	23.487878391392343	25.58722053465447	27.354392764401002	23.57050830955218
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	44.5
2	43.0
3	154.0
4	265.0
5	265.0
6	348.0
7	431.0
8	389.5
9	348.0
10	476.5
11	605.0
12	605.0
13	994.0
14	1383.0
15	2453.0
16	3523.0
17	4012.5
18	4502.0
19	4502.0
20	4445.0
21	4388.0
22	4955.0
23	5522.0
24	7390.5
25	9259.0
26	9259.0
27	12564.0
28	15869.0
29	18867.0
30	21865.0
31	27082.5
32	32300.0
33	32300.0
34	40257.5
35	48215.0
36	52901.0
37	57587.0
38	61934.5
39	66282.0
40	66282.0
41	68995.0
42	71708.0
43	72826.5
44	73945.0
45	70150.0
46	66355.0
47	66355.0
48	62108.0
49	57861.0
50	58371.0
51	58881.0
52	55390.0
53	51899.0
54	51899.0
55	52769.0
56	53639.0
57	45044.0
58	36449.0
59	33334.0
60	30219.0
61	30219.0
62	27661.5
63	25104.0
64	20188.5
65	15273.0
66	12555.0
67	9837.0
68	9837.0
69	8261.0
70	6685.0
71	5931.5
72	5178.0
73	5429.5
74	5681.0
75	5681.0
76	4929.0
77	4177.0
78	3016.0
79	1855.0
80	1181.5
81	508.0
82	508.0
83	392.5
84	277.0
85	241.5
86	206.0
87	164.5
88	123.0
89	123.0
90	87.0
91	51.0
92	33.5
93	16.0
94	8.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	848361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.67700926200344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09467662598767	42.76926048158381
2	7.932752778563304	7.8815086650774555
3	2.0099420452858374	2.9954372879926403
4	0.9170735762746995	1.8222989017014746
5	0.5394708754096684	1.33996498371536
6	0.35896868767780343	1.0699494493523676
7	0.2545883389048923	0.8853031088843482
8	0.18563269810813882	0.737734181059896
9	0.15718245674188017	0.7027518923451752
>10	1.212974634081954	12.325629516237072
>50	0.17548977264828586	6.181246169021609
>100	0.14483309309826334	13.99019442798891
>500	0.012310812913224892	4.275314584447468
>1k	0.0041036043044082975	3.023406350592489
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2565	0.30234770339513484	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2196	0.25885206887162426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.2147670626066026	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1801	0.21229170129225647	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1717	0.20239025603487193	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1644	0.19378542860881157	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1599	0.1884810829352127	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1589	0.18730233945219074	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1347	0.15877674716305912	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1328	0.15653713454531737	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1230	0.1449854484117021	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1094	0.12895453704260332	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1079	0.12718642181807038	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1073	0.12647917572825718	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1072	0.126361301379955	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1034	0.12188207614447152	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1023	0.12058545831314736	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	992	0.11693135351577925	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	980	0.1155168613361529	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	976	0.11504536394294411	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	960	0.11315937437010896	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	954	0.11245212828029577	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	933	0.10997676696594964	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	918	0.10820865174141668	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	918	0.10820865174141668	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	896	0.10561541607876837	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	887	0.10455454694404857	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	885	0.1043187982474442	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	884	0.104200923899142	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	876	0.10325792911272441	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	874	0.10302218041612003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.1787434830219682E-4	0.0	0.0
4	0.0	0.0	2.3574869660439364E-4	2.3574869660439364E-4	0.0
5	0.0	0.0	2.3574869660439364E-4	2.3574869660439364E-4	0.0
6	0.0	0.0	3.5362304490659045E-4	2.3574869660439364E-4	0.0
7	0.0	0.0	0.001296617831324165	3.5362304490659045E-4	0.0
8	0.0	0.0	0.0016502408762307556	3.5362304490659045E-4	0.0
9	0.0	0.0	0.002003863921137346	9.429947864175746E-4	0.0
10	2.3574869660439364E-4	0.0	0.0023574869660439365	9.429947864175746E-4	0.0
11	2.3574869660439364E-4	0.0	0.0036541047973681017	9.429947864175746E-4	0.0
12	2.3574869660439364E-4	0.0	0.004007727842274692	9.429947864175746E-4	0.0
13	2.3574869660439364E-4	0.0	0.004125602190576889	9.429947864175746E-4	0.0
14	2.3574869660439364E-4	0.0	0.004597099583785676	9.429947864175746E-4	0.0
15	2.3574869660439364E-4	0.0	0.00518647132529666	9.429947864175746E-4	0.0
16	2.3574869660439364E-4	0.0	0.005422220021901054	9.429947864175746E-4	0.0
17	3.5362304490659045E-4	0.0	0.005422220021901054	9.429947864175746E-4	0.0
18	3.5362304490659045E-4	0.0	0.005422220021901054	0.0011787434830219682	0.0
19	3.5362304490659045E-4	0.0	0.005540094370203251	0.0011787434830219682	0.0
20	3.5362304490659045E-4	0.0	0.005657968718505447	0.0015323665279285588	0.0
21	3.5362304490659045E-4	0.0	0.006011591763412038	0.0017681152245329524	0.0
22	3.5362304490659045E-4	0.0	0.006011591763412038	0.0022396126177417395	0.0
23	3.5362304490659045E-4	0.0	0.006011591763412038	0.0023574869660439365	0.0
24	3.5362304490659045E-4	0.0	0.006011591763412038	0.0028289843592527236	0.0
25	3.5362304490659045E-4	0.0	0.006011591763412038	0.003182607404159314	0.0
26	3.5362304490659045E-4	0.0	0.006011591763412038	0.003300481752461511	0.0
27	3.5362304490659045E-4	0.0	0.006011591763412038	0.004479225235483479	0.0
28	3.5362304490659045E-4	0.0	0.006011591763412038	0.012141057875126272	0.0
29	3.5362304490659045E-4	0.0	0.006011591763412038	0.02451786444685694	0.0
30	4.714973932087873E-4	0.0	0.006011591763412038	0.04267051408539525	0.0
31	5.893717415109841E-4	0.0	0.006011591763412038	0.09500672473157064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTC	30	3.5986904E-4	30.833334	8
GGTATCA	595	0.0	23.941177	1
TTAGACT	80	2.7237547E-8	23.125002	4
AACGATT	130	1.8189894E-12	21.346153	22
GTATCAA	1430	0.0	20.958042	1
GTCCGGC	55	5.141569E-4	20.181818	15
CATGCTA	500	0.0	19.98	4
TTGCGGT	95	1.6748163E-7	19.473684	35
CGTGTCG	95	1.6748163E-7	19.473684	9
ACGATTA	135	5.638867E-11	19.185184	23
TACGTGA	135	5.638867E-11	19.185184	36
TACATGC	510	0.0	18.862745	2
GTGTTAC	70	1.2187194E-4	18.5	1
ACCTCGG	150	1.2732926E-11	18.5	36
GTCAACC	510	0.0	18.5	15
GGACCGT	100	2.8727118E-7	18.5	6
TAGGACC	250	0.0	18.5	4
GTTATCG	50	0.0070334	18.5	11
CGAACTA	525	0.0	18.32381	29
CAACCCA	510	0.0	18.137255	17
>>END_MODULE