FastQCFastQC Report
Fri 10 Feb 2017
ERR1633240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907417
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67650.7455227310045988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.521259795661752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26870.29611523698586206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.13742303703809824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG250.005495740629.61
TAACGCA250.005495740629.64
CGACGAC358.867951E-426.4285733
GGTATCA10350.025.0241551
TCTCGCG451.3229126E-424.66666620
GTATCAA29500.023.7050861
ACACGAA400.001930875723.12517
TAGACCG450.003825197220.5555555
GACCGTA450.003825197220.5555557
ACGTATT450.003825197220.55555516
CGCAATG450.003825197220.55555532
TTAACGG1450.020.41379435
AACGGCC1550.020.29032337
GCTCGGT555.1419483E-420.18181826
TCGGTAG555.1419483E-420.18181828
GCAACGC609.2347723E-418.53
CGTTATT609.2347723E-418.510
CTCGGTA701.21883844E-418.527
CGTAGGC609.2347723E-418.522
TAGGCTA902.1512005E-618.55
Produced by FastQC (version 0.11.5)