##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633240.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907417
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93666417975418	33.0	33.0	33.0	33.0	33.0
2	32.053247845257474	33.0	33.0	33.0	33.0	33.0
3	31.77687105266928	33.0	33.0	33.0	33.0	33.0
4	32.04967837278782	33.0	33.0	33.0	33.0	33.0
5	32.14261910455722	33.0	33.0	33.0	33.0	33.0
6	35.33419916091499	37.0	37.0	37.0	33.0	37.0
7	35.4714910564823	37.0	37.0	37.0	33.0	37.0
8	35.611849899219436	37.0	37.0	37.0	33.0	37.0
9	35.710728364136884	37.0	37.0	37.0	33.0	37.0
10	35.722010938741505	37.0	37.0	37.0	33.0	37.0
11	35.74200835999326	37.0	37.0	37.0	33.0	37.0
12	35.728442380956054	37.0	37.0	37.0	33.0	37.0
13	35.73550748994123	37.0	37.0	37.0	33.0	37.0
14	35.71149537643663	37.0	37.0	37.0	33.0	37.0
15	35.736457439082585	37.0	37.0	37.0	33.0	37.0
16	35.714727627981404	37.0	37.0	37.0	33.0	37.0
17	35.71764139309711	37.0	37.0	37.0	33.0	37.0
18	35.706139514688395	37.0	37.0	37.0	33.0	37.0
19	35.69171505493065	37.0	37.0	37.0	33.0	37.0
20	35.69980505104048	37.0	37.0	37.0	33.0	37.0
21	35.688354968002585	37.0	37.0	37.0	33.0	37.0
22	35.6030237476265	37.0	37.0	37.0	33.0	37.0
23	35.64322687364244	37.0	37.0	37.0	33.0	37.0
24	35.638623697814786	37.0	37.0	37.0	33.0	37.0
25	35.65989065666612	37.0	37.0	37.0	33.0	37.0
26	35.5495742310316	37.0	37.0	37.0	33.0	37.0
27	35.56905590263352	37.0	37.0	37.0	33.0	37.0
28	35.58667624697355	37.0	37.0	37.0	33.0	37.0
29	35.59348348113381	37.0	37.0	37.0	33.0	37.0
30	35.5991975023611	37.0	37.0	37.0	33.0	37.0
31	35.58348366847877	37.0	37.0	37.0	33.0	37.0
32	35.57573419938132	37.0	37.0	37.0	33.0	37.0
33	35.56554924582634	37.0	37.0	37.0	33.0	37.0
34	35.54481787314983	37.0	37.0	37.0	33.0	37.0
35	35.476174680439094	37.0	37.0	37.0	33.0	37.0
36	35.504165119234045	37.0	37.0	37.0	33.0	37.0
37	35.48345909322836	37.0	37.0	37.0	33.0	37.0
38	35.47157701475727	37.0	37.0	37.0	33.0	37.0
39	35.36592768264205	37.0	37.0	37.0	33.0	37.0
40	35.148492920013624	37.0	37.0	37.0	33.0	37.0
41	35.30780997049868	37.0	37.0	37.0	33.0	37.0
42	35.342758621449676	37.0	37.0	37.0	33.0	37.0
43	35.015479101669904	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	29.0
16	46.0
17	38.0
18	33.0
19	23.0
20	51.0
21	126.0
22	317.0
23	778.0
24	1681.0
25	3134.0
26	5097.0
27	7844.0
28	11371.0
29	15384.0
30	20720.0
31	27029.0
32	34866.0
33	46489.0
34	67260.0
35	139504.0
36	525594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06963281490208	20.466114256179903	13.912126398337257	23.552126530580757
2	15.749209018565885	22.15365151854109	39.516451642409166	22.58068782048386
3	17.88064362911429	27.828440507506468	30.40641733624122	23.88449852713802
4	12.013770956462134	17.941365436177634	40.53274293957464	29.512120667785595
5	13.423817274747993	37.26599788189994	35.198040151330645	14.112144692021419
6	28.349810506084854	40.89211465070635	17.30406196930408	13.454012873904722
7	25.790017158594118	32.96136175540022	23.514988147676316	17.733632938329347
8	24.52676112525994	35.42120105750719	20.897338268954627	19.154699548278245
9	26.2714937013523	15.260018271643577	20.674287565694712	37.79420046130941
10	14.789892629298325	28.40788744314907	33.81719760595184	22.985022321600766
11	33.04258130495682	24.36288938823055	24.40862359863216	18.18590570818047
12	22.984140698267723	26.253420423024913	30.6768552936522	20.08558358505516
13	28.343418736920288	22.76737156125574	26.392496503812467	22.4967131980115
14	21.91153571070412	21.580155540396532	28.99901588795449	27.509292860944857
15	23.587060855152593	29.039350155441213	25.488942790359893	21.884646199046305
16	21.859189325304683	29.060949927100772	25.931076891881023	23.148783855713525
17	21.735321247012124	27.65839740714578	27.14419059814837	23.46209074769373
18	22.98436110410098	26.7109829328743	28.825225888428363	21.479430074596355
19	24.600266470652414	26.216943257620258	28.781034518859578	20.401755752867757
20	24.291588101170685	26.13836857806279	28.58200805142509	20.988035269341438
21	22.84396258831386	26.99166976153191	28.57032654226227	21.59404110789196
22	22.19222253936173	26.965110858623987	28.839552267590314	22.003114334423977
23	22.04675468940961	26.77280676910395	29.117594226248794	22.062844315237648
24	22.17282682603478	26.97789439695311	28.5577634097664	22.291515367245708
25	22.2755359443343	27.19730840396422	28.89652717548823	21.63062847621325
26	22.386840890130998	26.95331914654453	28.780814113026317	21.879025850298152
27	22.28016446683278	27.039718233182757	28.725933060544378	21.95418423944008
28	22.042787384410918	26.905931892393465	28.840654296756618	22.210626426439
29	22.604822259225912	26.99409422569778	28.46409093063057	21.93699258444574
30	22.578373559234617	26.89182591906477	28.701468013052434	21.828332508648174
31	22.53076589925029	26.94428250738084	28.557432801016514	21.967518792352358
32	21.452540562938538	26.87672811948641	29.04585212752241	22.624879190052642
33	21.526486719997532	26.869895538655324	29.468149704050067	22.135468037297077
34	22.124337542717406	26.53565009251535	29.002211772536775	22.337800592230476
35	21.985922679429635	27.12898259565338	28.744226744705024	22.140867980211965
36	22.563385962572884	26.973596483204528	28.65859907848321	21.80441847573938
37	22.535945436331918	26.723766471203426	28.51731893936305	22.22296915310161
38	22.502553952592912	26.91100122655846	28.318512877761826	22.267931943086804
39	22.18770421977988	26.71561145537278	28.726263669294273	22.37042065555307
40	21.98129415693116	27.14110491648272	28.910853554650178	21.966747371935945
41	22.07805231773264	26.450132629210167	28.882972216742687	22.58884283631451
42	21.690138051193664	27.11068891149273	28.80274449343576	22.396428543877843
43	22.17337784061793	26.547000992928275	28.760646979282956	22.518974187170837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	93.0
2	123.0
3	454.0
4	785.0
5	785.0
6	910.0
7	1035.0
8	948.0
9	861.0
10	1186.5
11	1512.0
12	1512.0
13	2475.0
14	3438.0
15	6103.0
16	8768.0
17	10145.0
18	11522.0
19	11522.0
20	10834.5
21	10147.0
22	10940.0
23	11733.0
24	14835.0
25	17937.0
26	17937.0
27	21526.5
28	25116.0
29	28933.5
30	32751.0
31	36774.0
32	40797.0
33	40797.0
34	45203.0
35	49609.0
36	52438.5
37	55268.0
38	57282.5
39	59297.0
40	59297.0
41	61044.0
42	62791.0
43	62988.0
44	63185.0
45	63013.5
46	62842.0
47	62842.0
48	62369.5
49	61897.0
50	62378.0
51	62859.0
52	61199.5
53	59540.0
54	59540.0
55	56396.5
56	53253.0
57	47597.0
58	41941.0
59	38200.0
60	34459.0
61	34459.0
62	30062.0
63	25665.0
64	21385.0
65	17105.0
66	14423.5
67	11742.0
68	11742.0
69	9678.0
70	7614.0
71	6457.0
72	5300.0
73	4097.0
74	2894.0
75	2894.0
76	2161.5
77	1429.0
78	1095.0
79	761.0
80	609.5
81	458.0
82	458.0
83	388.5
84	319.0
85	291.5
86	264.0
87	224.5
88	185.0
89	185.0
90	147.5
91	110.0
92	73.5
93	37.0
94	20.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	907417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.47523386876315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2432295185015	57.777460900588295
2	7.484660731171197	9.80119823603564
3	1.6021466780913367	3.147027853202769
4	0.6343445994613829	1.6613544401248377
5	0.38871455588276504	1.2725588227308224
6	0.2646199923628941	1.039563353178649
7	0.1836485483826863	0.8417102158510676
8	0.14080748202859805	0.7375522253035298
9	0.10964013838628765	0.6460842332021166
>10	0.8039738715312331	10.351180052460228
>50	0.09145162239175106	4.148961849336613
>100	0.05038711180174722	6.1635555616302184
>500	0.0016965357190484199	0.6979223814793412
>1k	5.08960715714526E-4	0.9624064162242578
>5k	1.6965357190484198E-4	0.7514634586515586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6765	0.7455227310045988	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4730	0.521259795661752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2687	0.29611523698586206	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1247	0.13742303703809824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.204058332607831E-4	0.0	0.0
2	0.0	0.0	2.204058332607831E-4	0.0	0.0
3	0.0	0.0	5.510145831519577E-4	0.0	0.0
4	0.0	0.0	7.714204164127408E-4	2.204058332607831E-4	0.0
5	1.1020291663039154E-4	0.0	9.91826249673524E-4	2.204058332607831E-4	0.0
6	1.1020291663039154E-4	0.0	0.0015428408328254815	2.204058332607831E-4	0.0
7	1.1020291663039154E-4	0.0	0.003967304998694096	2.204058332607831E-4	0.0
8	1.1020291663039154E-4	0.0	0.004848928331737228	3.3060874989117463E-4	0.0
9	1.1020291663039154E-4	0.0	0.005950957498041143	9.91826249673524E-4	0.0
10	3.3060874989117463E-4	0.0	0.006501972081193101	0.001212232082934307	0.0
11	3.3060874989117463E-4	0.0	0.009477450830213672	0.0013224349995646985	0.0
12	3.3060874989117463E-4	0.0	0.010138668329996022	0.0013224349995646985	0.0
13	3.3060874989117463E-4	0.0	0.010579479996517588	0.0013224349995646985	0.0
14	3.3060874989117463E-4	0.0	0.011461103329560721	0.0015428408328254815	0.0
15	4.408116665215662E-4	0.0	0.012563132495864635	0.001653043749455873	0.0
16	5.510145831519577E-4	0.0	0.013334552912277376	0.0017632466660862647	0.0
17	7.714204164127408E-4	0.0	0.013444755828907768	0.001873449582716656	0.0
18	9.91826249673524E-4	0.0	0.013444755828907768	0.001983652499347048	0.0
19	9.91826249673524E-4	0.0	0.013665161662168551	0.001983652499347048	0.0
20	9.91826249673524E-4	0.0	0.01421617624532051	0.002093855415977439	0.0
21	9.91826249673524E-4	0.0	0.014767190828472467	0.002644869999129397	0.0
22	9.91826249673524E-4	0.0	0.014767190828472467	0.003746899165433312	0.0
23	0.001212232082934307	0.0	0.014767190828472467	0.004077507915324487	0.0
24	0.001212232082934307	0.0	0.014987596661733249	0.004959131248367619	0.0
25	0.001212232082934307	0.0	0.014987596661733249	0.005399942914889186	0.0
26	0.001212232082934307	0.0	0.014987596661733249	0.005840754581410751	0.0
27	0.001212232082934307	0.0	0.014987596661733249	0.007604001247497016	0.0
28	0.001212232082934307	0.0	0.014987596661733249	0.01807327832738421	0.0
29	0.001212232082934307	0.0	0.014987596661733249	0.03879142665389782	0.0
30	0.001212232082934307	0.0	0.014987596661733249	0.0677747937276908	0.0
31	0.001212232082934307	0.0	0.014987596661733249	0.1405087187037492	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	0.0054957406	29.6	1
TAACGCA	25	0.0054957406	29.6	4
CGACGAC	35	8.867951E-4	26.42857	33
GGTATCA	1035	0.0	25.024155	1
TCTCGCG	45	1.3229126E-4	24.666666	20
GTATCAA	2950	0.0	23.705086	1
ACACGAA	40	0.0019308757	23.125	17
TAGACCG	45	0.0038251972	20.555555	5
GACCGTA	45	0.0038251972	20.555555	7
ACGTATT	45	0.0038251972	20.555555	16
CGCAATG	45	0.0038251972	20.555555	32
TTAACGG	145	0.0	20.413794	35
AACGGCC	155	0.0	20.290323	37
GCTCGGT	55	5.1419483E-4	20.181818	26
TCGGTAG	55	5.1419483E-4	20.181818	28
GCAACGC	60	9.2347723E-4	18.5	3
CGTTATT	60	9.2347723E-4	18.5	10
CTCGGTA	70	1.21883844E-4	18.5	27
CGTAGGC	60	9.2347723E-4	18.5	22
TAGGCTA	90	2.1512005E-6	18.5	5
>>END_MODULE