FastQCFastQC Report
Fri 10 Feb 2017
ERR1633239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064718
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34590.32487475556908024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.21724062146033035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13660.12829688236697415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA303.5993027E-430.83333227
GGTATCA6750.026.0370371
ACCGTTC400.001931094123.1258
GTATCAA17350.022.391931
GACCGTT902.1519118E-618.57
AGACCGT801.616488E-518.56
TTCGCCG1306.9667294E-1018.524
TTTGCGT500.007034550418.49999827
CGCCGTT500.007034550418.49999825
GAGTCGC852.7228109E-517.41176422
TGCGGTA904.4454602E-516.44444536
CTCTAAT3050.016.377051
CGAGTCG700.00259232615.85714221
TAGACTG1403.471723E-815.8571425
AGACGAT700.00259232615.85714229
TACGAAA1059.3417275E-615.85714218
ACGAAAT1059.3417275E-615.85714219
TTAGCCG1353.9710358E-715.07407529
AGCGTGA750.004104140714.816
TCTAGTA750.004104140714.82
Produced by FastQC (version 0.11.5)