FastQCFastQC Report
Fri 10 Feb 2017
ERR1633238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644777
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25520.3957957557419077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.2726524054052797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10370.16083079886534415No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6610.10251606369333893No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6480.10049986274324302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGG303.5977404E-430.83333421
CTATCGC250.005494575529.632
TATTGCG250.005494575529.611
TATCGCC250.005494575529.633
CGCTCTC754.620233E-1027.13333329
GGTATCA4750.023.7578951
CAACCGT400.001930272723.1256
GCCGGCA802.7210262E-823.12515
GCCGCTC802.7210262E-823.12527
TTCGCCG802.7210262E-823.12524
GTATCAA12050.020.5726151
AGCTTCG909.462019E-820.55555521
CCGCTCT909.462019E-820.55555528
TAAGAGC555.139728E-420.1818184
TCGCCGC951.6732702E-719.47368425
TCGCCAT1103.8437065E-818.513
TTAGACC609.230819E-418.54
CCTAAGA801.6148771E-518.52
CCGTACA1409.276846E-1118.517
GCTTCGC1002.870056E-718.522
Produced by FastQC (version 0.11.5)