##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644777
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84967670993227	33.0	33.0	33.0	33.0	33.0
2	31.98189916823956	33.0	33.0	33.0	33.0	33.0
3	31.696983608286274	33.0	33.0	33.0	27.0	33.0
4	31.9713621918896	33.0	33.0	33.0	33.0	33.0
5	32.07902111892949	33.0	33.0	33.0	33.0	33.0
6	35.25571011372924	37.0	37.0	37.0	33.0	37.0
7	35.394553465771885	37.0	37.0	37.0	33.0	37.0
8	35.5270799051455	37.0	37.0	37.0	33.0	37.0
9	35.62151255395276	37.0	37.0	37.0	33.0	37.0
10	35.65636646468469	37.0	37.0	37.0	33.0	37.0
11	35.66959739568874	37.0	37.0	37.0	33.0	37.0
12	35.65466975403899	37.0	37.0	37.0	33.0	37.0
13	35.66094944453664	37.0	37.0	37.0	33.0	37.0
14	35.63692098198292	37.0	37.0	37.0	33.0	37.0
15	35.65487292505781	37.0	37.0	37.0	33.0	37.0
16	35.62539296531979	37.0	37.0	37.0	33.0	37.0
17	35.632637330425865	37.0	37.0	37.0	33.0	37.0
18	35.6159881633495	37.0	37.0	37.0	33.0	37.0
19	35.61607191323512	37.0	37.0	37.0	33.0	37.0
20	35.61634332490148	37.0	37.0	37.0	33.0	37.0
21	35.609637130356695	37.0	37.0	37.0	33.0	37.0
22	35.52193393995133	37.0	37.0	37.0	33.0	37.0
23	35.56589487528246	37.0	37.0	37.0	33.0	37.0
24	35.55675683220711	37.0	37.0	37.0	33.0	37.0
25	35.58281390310138	37.0	37.0	37.0	33.0	37.0
26	35.47442449094803	37.0	37.0	37.0	33.0	37.0
27	35.494788120544	37.0	37.0	37.0	33.0	37.0
28	35.519329938878094	37.0	37.0	37.0	33.0	37.0
29	35.52152294514227	37.0	37.0	37.0	33.0	37.0
30	35.5298157347424	37.0	37.0	37.0	33.0	37.0
31	35.51417156629346	37.0	37.0	37.0	33.0	37.0
32	35.50554222622705	37.0	37.0	37.0	33.0	37.0
33	35.5102647892217	37.0	37.0	37.0	33.0	37.0
34	35.48555857296399	37.0	37.0	37.0	33.0	37.0
35	35.43165466510127	37.0	37.0	37.0	33.0	37.0
36	35.46557491970092	37.0	37.0	37.0	33.0	37.0
37	35.468498411078556	37.0	37.0	37.0	33.0	37.0
38	35.451348295612284	37.0	37.0	37.0	33.0	37.0
39	35.36085964604817	37.0	37.0	37.0	33.0	37.0
40	35.15788249270678	37.0	37.0	37.0	33.0	37.0
41	35.32457423264167	37.0	37.0	37.0	33.0	37.0
42	35.37802992352395	37.0	37.0	37.0	33.0	37.0
43	35.03982462153582	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	20.0
16	28.0
17	27.0
18	21.0
19	11.0
20	33.0
21	73.0
22	261.0
23	638.0
24	1399.0
25	2404.0
26	3946.0
27	6115.0
28	8683.0
29	11731.0
30	15338.0
31	19909.0
32	25523.0
33	33898.0
34	49058.0
35	99308.0
36	366351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8279141470617	21.1549109226911	13.837187120508332	24.179987809738872
2	15.666967028910772	22.141143372049562	39.31793472782063	22.87395487121904
3	17.403226231704917	27.584110475404678	30.92914294399459	24.08352034889582
4	12.419642760210118	18.12704237278935	40.125810939285984	29.32750392771454
5	13.122831614651268	37.89465194943368	36.02749477726408	12.955021658650976
6	28.529708720999665	40.58116216924611	17.82430204551341	13.064827064240813
7	25.621416396676683	32.79552465426031	24.205888237328566	17.377170711734443
8	23.91912242527261	36.44764003678792	21.058753646609603	18.57448389132987
9	25.452365701630175	15.833691338245625	20.766404508845692	37.947538451278504
10	14.482991794062134	28.612372959953596	34.722392393028905	22.182242852955365
11	32.87632778464493	24.251950674419216	24.83277164042762	18.03894990050824
12	22.08779159306241	26.471012458570947	30.800571360330782	20.640624588035863
13	27.083627362638556	22.778107779899095	27.133101832106295	23.005163025356055
14	21.399491607175815	21.763493424858517	28.366396444041893	28.470618523923775
15	23.39553054777078	29.529744392247242	24.94350760030212	22.13121745967986
16	22.381536562253306	29.37201544099743	25.57969654624777	22.66675145050149
17	21.790944776255976	28.42657228778322	26.940322002800972	22.842160933159835
18	21.941694570370842	28.314905773624062	28.326227517420747	21.41717213858435
19	23.852897978681	27.181645747289373	28.237049398474202	20.728406875555425
20	23.534958598088952	26.835169368634425	28.493882381040265	21.135989652236354
21	22.17650443486663	27.86079528271015	27.826985143700846	22.135715138722382
22	21.867405319978843	27.82124672561211	27.918179463597493	22.39316849081155
23	21.94138438560929	27.745406551412348	28.442236618241658	21.870972444736708
24	22.57617750012795	27.95571181974543	27.28338634907883	22.18472433104779
25	22.48389753356587	27.554332738295567	28.120264835749413	21.841504892389153
26	22.414881424120278	27.640719194388137	28.021936266337043	21.922463115154542
27	22.254981179539595	27.628466896306787	27.514629088816754	22.601922835336868
28	21.752481865823377	27.66537888293162	28.44316717252632	22.138972078718687
29	22.97693621205471	27.670962208639576	27.617920614413975	21.73418096489174
30	22.383862947964957	27.717489922872556	27.982077524477454	21.91656960468503
31	22.496460016408772	27.50935594787035	28.074357491039535	21.91982654468134
32	21.64670886213373	27.43149957272049	28.23394755085867	22.68784401428711
33	21.7039379506403	27.343562192820155	28.730708446486148	22.2217914100534
34	22.22861547480757	27.36480984898655	28.228519317531486	22.178055358674396
35	22.36618241655642	27.957417835933974	27.700274668606355	21.976125078903248
36	22.81812161413946	27.661036296269874	27.420643106066127	22.100198983524535
37	22.726462017100484	26.755762069676802	27.871651749364506	22.646124163858204
38	22.63108020292287	27.509666132631907	27.799223607541833	22.060030056903397
39	22.09197908734338	27.460501847925716	28.06288065486207	22.38463840986884
40	22.16642343011615	27.872582303649168	28.09172783768652	21.869266428548165
41	22.11787951493307	26.98840064084172	28.238290137520416	22.6554297067048
42	22.035370368359914	27.624434494406593	27.90080911695051	22.43938602028298
43	22.42883973839017	26.88355819143673	28.005961751117052	22.681640319056047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	35.5
2	40.0
3	151.5
4	263.0
5	263.0
6	329.0
7	395.0
8	368.5
9	342.0
10	483.0
11	624.0
12	624.0
13	1033.5
14	1443.0
15	2703.5
16	3964.0
17	4670.0
18	5376.0
19	5376.0
20	5661.5
21	5947.0
22	7164.5
23	8382.0
24	10211.0
25	12040.0
26	12040.0
27	14764.5
28	17489.0
29	20666.5
30	23844.0
31	26470.5
32	29097.0
33	29097.0
34	32933.5
35	36770.0
36	39050.5
37	41331.0
38	44533.0
39	47735.0
40	47735.0
41	49498.5
42	51262.0
43	50048.0
44	48834.0
45	48454.0
46	48074.0
47	48074.0
48	46464.0
49	44854.0
50	45147.0
51	45440.0
52	44462.0
53	43484.0
54	43484.0
55	39454.5
56	35425.0
57	30900.0
58	26375.0
59	23489.0
60	20603.0
61	20603.0
62	18210.5
63	15818.0
64	13116.5
65	10415.0
66	8773.0
67	7131.0
68	7131.0
69	5788.0
70	4445.0
71	3851.0
72	3257.0
73	2566.0
74	1875.0
75	1875.0
76	1411.5
77	948.0
78	764.0
79	580.0
80	473.0
81	366.0
82	366.0
83	279.0
84	192.0
85	179.5
86	167.0
87	120.0
88	73.0
89	73.0
90	55.0
91	37.0
92	21.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.45719671368103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68796770999617	50.67535581132475
2	7.6227633578292915	8.912107542209332
3	1.9461384450548982	3.4129739374389447
4	0.8830763221657033	2.064886651121379
5	0.5301685402030578	1.5496083323027632
6	0.35803784039934666	1.2557933080299684
7	0.24226200221276706	0.9913370263721367
8	0.194879690038918	0.911369630088497
9	0.15371285922505082	0.8087060564227039
>10	1.1387697125224556	13.280988331370924
>50	0.16255891297767616	6.599210786447528
>100	0.07670409831679835	7.984500285106478
>500	0.002153097496611108	0.7119184983270774
>1k	8.074115612291654E-4	0.8412438034375432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2552	0.3957957557419077	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1758	0.2726524054052797	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1037	0.16083079886534415	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	661	0.10251606369333893	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	648	0.10049986274324302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.5509238077660958E-4	0.0	0.0
2	0.0	0.0	1.5509238077660958E-4	0.0	0.0
3	0.0	0.0	1.5509238077660958E-4	0.0	0.0
4	0.0	0.0	1.5509238077660958E-4	0.0	0.0
5	0.0	0.0	3.1018476155321916E-4	0.0	0.0
6	0.0	0.0	9.305542846596575E-4	1.5509238077660958E-4	0.0
7	0.0	0.0	0.002326385711649144	1.5509238077660958E-4	0.0
8	0.0	0.0	0.002636570473202363	1.5509238077660958E-4	0.0
9	0.0	0.0	0.0032569399963088014	9.305542846596575E-4	0.0
10	1.5509238077660958E-4	0.0	0.0035671247578620206	0.001085646665436267	0.0
11	1.5509238077660958E-4	0.0	0.005428233327181335	0.0012407390462128767	0.0
12	1.5509238077660958E-4	0.0	0.006203695231064383	0.0012407390462128767	0.0
13	1.5509238077660958E-4	0.0	0.006979157134947431	0.0012407390462128767	0.0
14	1.5509238077660958E-4	0.0	0.007289341896500651	0.0012407390462128767	0.0
15	1.5509238077660958E-4	0.0	0.0080648038003837	0.0012407390462128767	0.0
16	1.5509238077660958E-4	0.0	0.008530080942713528	0.0013958314269894862	0.0
17	1.5509238077660958E-4	0.0	0.008685173323490137	0.0017060161885427054	0.0
18	1.5509238077660958E-4	0.0	0.008685173323490137	0.0017060161885427054	0.0
19	3.1018476155321916E-4	0.0	0.008840265704266747	0.0017060161885427054	0.0
20	3.1018476155321916E-4	0.0	0.009305542846596576	0.0017060161885427054	0.0
21	3.1018476155321916E-4	0.0	0.009305542846596576	0.002016200950095925	0.0
22	3.1018476155321916E-4	0.0	0.009305542846596576	0.0024814780924257533	0.0
23	3.1018476155321916E-4	0.0	0.009305542846596576	0.0027916628539789725	0.0
24	3.1018476155321916E-4	0.0	0.009615727608149794	0.003412032377085411	0.0
25	3.1018476155321916E-4	0.0	0.009615727608149794	0.0035671247578620206	0.0
26	3.1018476155321916E-4	0.0	0.009615727608149794	0.00372221713863863	0.0
27	3.1018476155321916E-4	0.0	0.009615727608149794	0.005583325707957945	0.0
28	3.1018476155321916E-4	0.0	0.009615727608149794	0.013958314269894863	0.0
29	3.1018476155321916E-4	0.0	0.009615727608149794	0.03148375329765175	0.0
30	3.1018476155321916E-4	0.0	0.009615727608149794	0.05645362660268589	0.0
31	3.1018476155321916E-4	0.0	0.009615727608149794	0.10825448178207349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGG	30	3.5977404E-4	30.833334	21
CTATCGC	25	0.0054945755	29.6	32
TATTGCG	25	0.0054945755	29.6	11
TATCGCC	25	0.0054945755	29.6	33
CGCTCTC	75	4.620233E-10	27.133333	29
GGTATCA	475	0.0	23.757895	1
CAACCGT	40	0.0019302727	23.125	6
GCCGGCA	80	2.7210262E-8	23.125	15
GCCGCTC	80	2.7210262E-8	23.125	27
TTCGCCG	80	2.7210262E-8	23.125	24
GTATCAA	1205	0.0	20.572615	1
AGCTTCG	90	9.462019E-8	20.555555	21
CCGCTCT	90	9.462019E-8	20.555555	28
TAAGAGC	55	5.139728E-4	20.181818	4
TCGCCGC	95	1.6732702E-7	19.473684	25
TCGCCAT	110	3.8437065E-8	18.5	13
TTAGACC	60	9.230819E-4	18.5	4
CCTAAGA	80	1.6148771E-5	18.5	2
CCGTACA	140	9.276846E-11	18.5	17
GCTTCGC	100	2.870056E-7	18.5	22
>>END_MODULE