FastQCFastQC Report
Fri 10 Feb 2017
ERR1633237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487461
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32170.6599502319159891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.47367891995462197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12570.25786678318880896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.14072920705451308No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6500.13334400085340162No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5850.12000960076806146No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4880.10011057294839996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGC250.005493277729.5999986
AGCGTCA509.077072E-625.89999837
GTATCAA14600.024.3287681
GGTATCA5300.023.0377371
TTAACGG903.814421E-922.6111135
TCGCACC603.7206224E-521.58333228
TTCGCCG603.7206224E-521.58333224
GTCGCCA806.94321E-720.812512
GCACCGC909.450196E-820.55555510
AACGGCC1001.2852979E-820.34999837
GTATTAG1850.020.01
CTGAGCG656.8917114E-519.92307734
ACGGGTA656.8917114E-519.92307715
TTCGCAC656.8917114E-519.92307727
TCGCCAT851.2422988E-619.58823613
TTTCGCA701.21737045E-418.526
GTCTAGG609.226418E-418.51
CCCACGC902.147046E-618.519
CTTTCGC701.21737045E-418.525
TGAGCGT701.21737045E-418.535
Produced by FastQC (version 0.11.5)